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The map label for this gene is pycA [H]

Identifier: 45357904

GI number: 45357904

Start: 337634

End: 339139

Strand: Direct

Name: pycA [H]

Synonym: MMP0341

Alternate gene names: 45357904

Gene position: 337634-339139 (Clockwise)

Preceding gene: 45357903

Following gene: 45357905

Centisome position: 20.33

GC content: 37.38

Gene sequence:

>1506_bases
ATGTTTAAAAAAGTATTGATAGCCAATAGGGGAGAAATTGCAGTCAGGGTAATTCGAGCATGTAAAGAACTTGGAATTAA
GACTGTTGCAGTTTTTTCTGAAGCTGACGAACACTCACTCTATAGAAATATTGCAGATGAATGTTATCCAATCGGGGAAC
CTCCTGCATCAAAAAGCTACCTTAACATGGAAAGGATCTTAAAAGTGGCTGAAAAAGCAGGTGTGGATGCAGTTCACCCA
GGATATGGTTTTTTATCTGAAAATGTAAAATTTGCAGATGAATGTCACAAAAGAGGCATTGAATTTATCGGCCCTCCAAC
AAACGCAATTGATGTTATGGGCAGTAAAATCAATGCTAAGAAAGCAATGAAAACAGCAGGAGTTCCTGTACTGCCTGGAA
GGGAAGAATCAATCGAAAGCGAAGAAGAAGCAATCGAAGTTGCAGATGAAATTGGATACCCTGTAATCATAAAAGCATCT
GCAGGTGGTGGCGGTATCGGAATGAACGTGGTTTACAATAAAGAAGAACTCGTTGATACGATACAGTCTACAAAATCCAT
TGCACAGAGTGCTTTTGGTGATTCAACAGTATTTATTGAAAAATACCTCGAAAAACCAAGACACATTGAAATACAGGTGG
TTGCAGATAAATTTGGAAATGTGATTCACCTTGGTGATAGGGAGTGTTCAATTCAAAGAAGGCACCAAAAATTAATTGAA
GAGTCCCCCTCTCCAATCATGACCGAGGAATTAAGGGAAAGAATGGGAAATGCAGCTGTAAAAGCTGCTAAGGCCATAAA
TTACCACAGCGTGGGTACGGTTGAATTTTTATACAGCAAAGGCGAATTTTATTTCCTTGAAATGAATACAAGAGTTCAGG
TAGAACACCCAATAACAGAGGTTGTTACTGGCGTTGATATCGTAAAAGAACAGATAAAAATTGCAGCCGGAAAAAAATTA
TCCTACACCCAAGATGAAATTACATTCAGAGGACATGCAATAGAATGTAGGGTAAATGCAGAAGATGCAATAAATGATTT
TGTTCCAGCTCCTGGAAAAATAAAATATTACCGGTCTCCTGGAGGTCCAGGTGTAAGGCTCGATAGTGGTGTATTTGGGG
GAGCTGAAATTCCACCTTATTATGATTCCATGGTTGCAAAGATAATTACGTACGGTCTTACAAGAGAAGAAGCAATTGAA
AGGATGAAAAGGGCACTTTCCGAATATATGGTTCTAGGAATAATTACAAACATACCTTTCCACAGGGCGGTAATTGAGGA
AGACAACTTTTTAAAAGGAAACCTTTCAACGCACTACGTTGAAGATAATCTATGCTCATTTAGAAAAGCAATGGTAAATT
ATGCTTTAGAAGCAAAAGATAGGGAAAAAATATTCAGTGAAAAAGTATTCCATGGTAATAAAAAGGTCATTGCAATTGCA
GGCGGTTTAAATGCTTACATAACAAGCCTTTCAAATAAAAATAAGGATAAATACGATAAAAATTAA

Upstream 100 bases:

>100_bases
CGTTAAAGAAGGTCAATCTGTAAACGTGGGCGACATATTGATGATTATAAAATAAATTAAGCACTTAATTAATGATACAG
CAATTCCAGAAGGTAATATT

Downstream 100 bases:

>100_bases
GTGCTTGATTGTACTTACTTTAAATATATTAAATCATCCACTTAATTTTATGAATAATAGAAAAATATAAATACTAAATA
AATTATCTTTTTATAGTGAT

Product: pyruvate carboxylase subunit A

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MFKKVLIANRGEIAVRVIRACKELGIKTVAVFSEADEHSLYRNIADECYPIGEPPASKSYLNMERILKVAEKAGVDAVHP
GYGFLSENVKFADECHKRGIEFIGPPTNAIDVMGSKINAKKAMKTAGVPVLPGREESIESEEEAIEVADEIGYPVIIKAS
AGGGGIGMNVVYNKEELVDTIQSTKSIAQSAFGDSTVFIEKYLEKPRHIEIQVVADKFGNVIHLGDRECSIQRRHQKLIE
ESPSPIMTEELRERMGNAAVKAAKAINYHSVGTVEFLYSKGEFYFLEMNTRVQVEHPITEVVTGVDIVKEQIKIAAGKKL
SYTQDEITFRGHAIECRVNAEDAINDFVPAPGKIKYYRSPGGPGVRLDSGVFGGAEIPPYYDSMVAKIITYGLTREEAIE
RMKRALSEYMVLGIITNIPFHRAVIEEDNFLKGNLSTHYVEDNLCSFRKAMVNYALEAKDREKIFSEKVFHGNKKVIAIA
GGLNAYITSLSNKNKDKYDKN

Sequences:

>Translated_501_residues
MFKKVLIANRGEIAVRVIRACKELGIKTVAVFSEADEHSLYRNIADECYPIGEPPASKSYLNMERILKVAEKAGVDAVHP
GYGFLSENVKFADECHKRGIEFIGPPTNAIDVMGSKINAKKAMKTAGVPVLPGREESIESEEEAIEVADEIGYPVIIKAS
AGGGGIGMNVVYNKEELVDTIQSTKSIAQSAFGDSTVFIEKYLEKPRHIEIQVVADKFGNVIHLGDRECSIQRRHQKLIE
ESPSPIMTEELRERMGNAAVKAAKAINYHSVGTVEFLYSKGEFYFLEMNTRVQVEHPITEVVTGVDIVKEQIKIAAGKKL
SYTQDEITFRGHAIECRVNAEDAINDFVPAPGKIKYYRSPGGPGVRLDSGVFGGAEIPPYYDSMVAKIITYGLTREEAIE
RMKRALSEYMVLGIITNIPFHRAVIEEDNFLKGNLSTHYVEDNLCSFRKAMVNYALEAKDREKIFSEKVFHGNKKVIAIA
GGLNAYITSLSNKNKDKYDKN
>Mature_501_residues
MFKKVLIANRGEIAVRVIRACKELGIKTVAVFSEADEHSLYRNIADECYPIGEPPASKSYLNMERILKVAEKAGVDAVHP
GYGFLSENVKFADECHKRGIEFIGPPTNAIDVMGSKINAKKAMKTAGVPVLPGREESIESEEEAIEVADEIGYPVIIKAS
AGGGGIGMNVVYNKEELVDTIQSTKSIAQSAFGDSTVFIEKYLEKPRHIEIQVVADKFGNVIHLGDRECSIQRRHQKLIE
ESPSPIMTEELRERMGNAAVKAAKAINYHSVGTVEFLYSKGEFYFLEMNTRVQVEHPITEVVTGVDIVKEQIKIAAGKKL
SYTQDEITFRGHAIECRVNAEDAINDFVPAPGKIKYYRSPGGPGVRLDSGVFGGAEIPPYYDSMVAKIITYGLTREEAIE
RMKRALSEYMVLGIITNIPFHRAVIEEDNFLKGNLSTHYVEDNLCSFRKAMVNYALEAKDREKIFSEKVFHGNKKVIAIA
GGLNAYITSLSNKNKDKYDKN

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI116805327, Length=458, Percent_Identity=46.5065502183406, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI189095269, Length=444, Percent_Identity=50.9009009009009, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI295821183, Length=444, Percent_Identity=50.9009009009009, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI65506442, Length=444, Percent_Identity=50.9009009009009, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI106049528, Length=456, Percent_Identity=46.7105263157895, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI106049295, Length=456, Percent_Identity=46.7105263157895, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI106049292, Length=456, Percent_Identity=46.7105263157895, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI38679960, Length=502, Percent_Identity=32.8685258964143, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI38679977, Length=502, Percent_Identity=32.8685258964143, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI38679967, Length=502, Percent_Identity=32.8685258964143, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI38679974, Length=502, Percent_Identity=32.8685258964143, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI38679971, Length=502, Percent_Identity=32.8685258964143, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI134142062, Length=505, Percent_Identity=31.8811881188119, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI18105007, Length=357, Percent_Identity=23.249299719888, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI170295797, Length=313, Percent_Identity=23.6421725239617, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI169790915, Length=313, Percent_Identity=23.6421725239617, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI21361331, Length=313, Percent_Identity=23.6421725239617, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1789654, Length=445, Percent_Identity=52.8089887640449, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1786216, Length=314, Percent_Identity=24.203821656051, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17567343, Length=501, Percent_Identity=46.3073852295409, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71987519, Length=443, Percent_Identity=46.27539503386, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17562816, Length=473, Percent_Identity=43.5517970401691, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71997168, Length=504, Percent_Identity=30.952380952381, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI71997163, Length=504, Percent_Identity=30.952380952381, Blast_Score=214, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI133931226, Length=449, Percent_Identity=30.7349665924276, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI193204318, Length=433, Percent_Identity=22.8637413394919, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319695, Length=454, Percent_Identity=46.2555066079295, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321376, Length=454, Percent_Identity=46.4757709251101, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=43.8636363636364, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324343, Length=511, Percent_Identity=31.3111545988258, Blast_Score=240, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6323863, Length=502, Percent_Identity=33.2669322709163, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=24.7588424437299, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24651757, Length=497, Percent_Identity=43.4607645875252, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24652212, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24652210, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24652214, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI19921944, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24652216, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI281363050, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24652224, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24652222, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24652220, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24652218, Length=452, Percent_Identity=47.3451327433628, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651759, Length=461, Percent_Identity=41.648590021692, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI161076409, Length=499, Percent_Identity=32.2645290581162, Blast_Score=242, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24586458, Length=499, Percent_Identity=32.2645290581162, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI161076407, Length=499, Percent_Identity=32.2645290581162, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24586460, Length=499, Percent_Identity=32.2645290581162, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI45555749, Length=357, Percent_Identity=24.0896358543417, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24642586, Length=357, Percent_Identity=24.0896358543417, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 55613; Mature: 55613

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKVLIANRGEIAVRVIRACKELGIKTVAVFSEADEHSLYRNIADECYPIGEPPASKSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHH
LNMERILKVAEKAGVDAVHPGYGFLSENVKFADECHKRGIEFIGPPTNAIDVMGSKINAK
HCHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHCCCCEECCCCCHHHHCCCCCCHH
KAMKTAGVPVLPGREESIESEEEAIEVADEIGYPVIIKASAGGGGIGMNVVYNKEELVDT
HHHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHH
IQSTKSIAQSAFGDSTVFIEKYLEKPRHIEIQVVADKFGNVIHLGDRECSIQRRHQKLIE
HHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECHHCCEEEECCCCHHHHHHHHHHHH
ESPSPIMTEELRERMGNAAVKAAKAINYHSVGTVEFLYSKGEFYFLEMNTRVQVEHPITE
CCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCEEEECCCHHH
VVTGVDIVKEQIKIAAGKKLSYTQDEITFRGHAIECRVNAEDAINDFVPAPGKIKYYRSP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEECCHHHHHHCCCCCCCEEEEECC
GGPGVRLDSGVFGGAEIPPYYDSMVAKIITYGLTREEAIERMKRALSEYMVLGIITNIPF
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
HRAVIEEDNFLKGNLSTHYVEDNLCSFRKAMVNYALEAKDREKIFSEKVFHGNKKVIAIA
HHHEECCCCCEECCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEE
GGLNAYITSLSNKNKDKYDKN
CCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFKKVLIANRGEIAVRVIRACKELGIKTVAVFSEADEHSLYRNIADECYPIGEPPASKSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHH
LNMERILKVAEKAGVDAVHPGYGFLSENVKFADECHKRGIEFIGPPTNAIDVMGSKINAK
HCHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHCCCCEECCCCCHHHHCCCCCCHH
KAMKTAGVPVLPGREESIESEEEAIEVADEIGYPVIIKASAGGGGIGMNVVYNKEELVDT
HHHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHH
IQSTKSIAQSAFGDSTVFIEKYLEKPRHIEIQVVADKFGNVIHLGDRECSIQRRHQKLIE
HHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECHHCCEEEECCCCHHHHHHHHHHHH
ESPSPIMTEELRERMGNAAVKAAKAINYHSVGTVEFLYSKGEFYFLEMNTRVQVEHPITE
CCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCEEEECCCHHH
VVTGVDIVKEQIKIAAGKKLSYTQDEITFRGHAIECRVNAEDAINDFVPAPGKIKYYRSP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEEEEEECCHHHHHHCCCCCCCEEEEECC
GGPGVRLDSGVFGGAEIPPYYDSMVAKIITYGLTREEAIERMKRALSEYMVLGIITNIPF
CCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
HRAVIEEDNFLKGNLSTHYVEDNLCSFRKAMVNYALEAKDREKIFSEKVFHGNKKVIAIA
HHHEECCCCCEECCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEE
GGLNAYITSLSNKNKDKYDKN
CCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]