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The map label for this gene is pycB [H]

Identifier: 45357903

GI number: 45357903

Start: 335879

End: 337588

Strand: Direct

Name: pycB [H]

Synonym: MMP0340

Alternate gene names: 45357903

Gene position: 335879-337588 (Clockwise)

Preceding gene: 45357902

Following gene: 45357904

Centisome position: 20.22

GC content: 36.26

Gene sequence:

>1710_bases
ATGGTAAAAATAACCGATACCTCCTTTAGGGATGCGCATCAATCTTTAATGGCAACAAGGTTAAGAACCGAAGACATGTT
ACCGATTGCAGAGAAAATGGACGAAGTAGGATTTTATTCGATGGAAGTATGGGGCGGTGCTACTTTTGATTCATGTATTA
GGTATTTAAATGAGGACCCATGGGAAAGATTAAGGGAATTAAAAGGAAAAATTCAAAACACGCCCTTACAAATGCTTTTA
AGGGGACAAAACTTAGTAGGATACAGGCACTACCCTGATGATGTTGTAGACAAGTTTATTCAAAAATCTATCGAGAATGG
GATAGATATTGTAAGAATATTCGATGCTCTAAACGATATTAGAAATGTTGAATATTCTATAAAAGCTGTTAAAAAATACG
GTGCTGAAGTTCAGGGTGCTATATCGTATACTACAAGCCCAGTTCACACGACCGAACAGTTTGTAGAGCTTGCAAAACAG
TTTGAAGAGTTGGGTTGTGATTCTTTATGTATTAAGGACATGGCAGGACTTTTAAGGCCCTTTGATGCTAAAGAACTTGT
TGGAATGTTAAAAGAAGAATTATCAATTCCAATCGATTTACACAGCCACTGTACTGCAGGAATTGCTCCATTAGCATATA
TGTCTTCTGTAGAAGCAGGAATTGATATATTAAATTGTGCAATGTCCCCTCTTTCAATGGGTACATCTCAGCCACCTGTA
GAAAGTATTGTGGCAGCATTAAAAGGCACAAAATACGATACAAACTTAGATCTTGTATTATTAACCGAAATTAGGGACTA
TTTTGACGAGATTAGAAACAAATACAAATGCCTGATAAATCCAATCTCTGAAAGAGTTGACGCTAGAATTTTAAGATATC
AGGTTCCGGGAGGAATGCTTTCAAACCTAGTATCCCAATTAAAAGAACAGGGTGCACTCGATAAATTTGAGGAAGTTTTA
AACGAAATTCCAAACGTGAGAAAAGATCTTGGATATCCTCCATTAGTAACTCCAACGTCCCAGATTGTTGGAACTCAGGC
TGTAATGAATGTATTGACTGATGAAAGATACAAAATCATCACAAATGAAGTAGCTAACTACTTAAAAGGATACTATGGAA
AATCACCTGCACCAATCAGCAAAGATCTCGTTAAACGGGCACTTCAAGATGGTGAAAAGCAGATTACATGCAGACCTGCA
GATTTACTCGAACCAGAATACGAATTAATGGAAAAAGAAGCTTTCGCAAAAGGAATAGTTTCAAAAGAAGAAGATATTTT
AACTTACGCTTTATACCCGCAAGTTGCAGTCAAATTCTTGAGAGGAGAATTAAAAGCAGAAACAATACCTCAGGAAAAGG
ACGTTTCAAAATTCATGGAAATTCCAAACGAATACATTGTTGAAGTTGATGGGGATTCTTTCAACGTAAAAGTGGAACCA
GTATACAGTTCAGGAATTAAAAAAGAAGAGAAAAAAGAGATAATAACCGCAGAAACCGAGGGTGCAGTAACTTCACCATT
TAGGGGTATTGTTACAAAAATAAAAGTTAGTACGGGCGCAAGTGTTAAACAAGGCGATCAAATTTTAGTTTTAGAAGCTA
TGAAGATGGAAAACCCTGTAGAATGTCCTGTAGATGGAACTGTTGAAAAAATAATCGTTAAAGAAGGTCAATCTGTAAAC
GTGGGCGACATATTGATGATTATAAAATAA

Upstream 100 bases:

>100_bases
ATATCTAATTTAATATAACCAAAAGAGAATCTAATCAAAACAAGAGGAAACTCGTTTCTATCTTTTTTACTAATATTATG
TTATTAAGCGAGGGATAACC

Downstream 100 bases:

>100_bases
ATTAAGCACTTAATTAATGATACAGCAATTCCAGAAGGTAATATTATGTTTAAAAAAGTATTGATAGCCAATAGGGGAGA
AATTGCAGTCAGGGTAATTC

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MVKITDTSFRDAHQSLMATRLRTEDMLPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDPWERLRELKGKIQNTPLQMLL
RGQNLVGYRHYPDDVVDKFIQKSIENGIDIVRIFDALNDIRNVEYSIKAVKKYGAEVQGAISYTTSPVHTTEQFVELAKQ
FEELGCDSLCIKDMAGLLRPFDAKELVGMLKEELSIPIDLHSHCTAGIAPLAYMSSVEAGIDILNCAMSPLSMGTSQPPV
ESIVAALKGTKYDTNLDLVLLTEIRDYFDEIRNKYKCLINPISERVDARILRYQVPGGMLSNLVSQLKEQGALDKFEEVL
NEIPNVRKDLGYPPLVTPTSQIVGTQAVMNVLTDERYKIITNEVANYLKGYYGKSPAPISKDLVKRALQDGEKQITCRPA
DLLEPEYELMEKEAFAKGIVSKEEDILTYALYPQVAVKFLRGELKAETIPQEKDVSKFMEIPNEYIVEVDGDSFNVKVEP
VYSSGIKKEEKKEIITAETEGAVTSPFRGIVTKIKVSTGASVKQGDQILVLEAMKMENPVECPVDGTVEKIIVKEGQSVN
VGDILMIIK

Sequences:

>Translated_569_residues
MVKITDTSFRDAHQSLMATRLRTEDMLPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDPWERLRELKGKIQNTPLQMLL
RGQNLVGYRHYPDDVVDKFIQKSIENGIDIVRIFDALNDIRNVEYSIKAVKKYGAEVQGAISYTTSPVHTTEQFVELAKQ
FEELGCDSLCIKDMAGLLRPFDAKELVGMLKEELSIPIDLHSHCTAGIAPLAYMSSVEAGIDILNCAMSPLSMGTSQPPV
ESIVAALKGTKYDTNLDLVLLTEIRDYFDEIRNKYKCLINPISERVDARILRYQVPGGMLSNLVSQLKEQGALDKFEEVL
NEIPNVRKDLGYPPLVTPTSQIVGTQAVMNVLTDERYKIITNEVANYLKGYYGKSPAPISKDLVKRALQDGEKQITCRPA
DLLEPEYELMEKEAFAKGIVSKEEDILTYALYPQVAVKFLRGELKAETIPQEKDVSKFMEIPNEYIVEVDGDSFNVKVEP
VYSSGIKKEEKKEIITAETEGAVTSPFRGIVTKIKVSTGASVKQGDQILVLEAMKMENPVECPVDGTVEKIIVKEGQSVN
VGDILMIIK
>Mature_569_residues
MVKITDTSFRDAHQSLMATRLRTEDMLPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDPWERLRELKGKIQNTPLQMLL
RGQNLVGYRHYPDDVVDKFIQKSIENGIDIVRIFDALNDIRNVEYSIKAVKKYGAEVQGAISYTTSPVHTTEQFVELAKQ
FEELGCDSLCIKDMAGLLRPFDAKELVGMLKEELSIPIDLHSHCTAGIAPLAYMSSVEAGIDILNCAMSPLSMGTSQPPV
ESIVAALKGTKYDTNLDLVLLTEIRDYFDEIRNKYKCLINPISERVDARILRYQVPGGMLSNLVSQLKEQGALDKFEEVL
NEIPNVRKDLGYPPLVTPTSQIVGTQAVMNVLTDERYKIITNEVANYLKGYYGKSPAPISKDLVKRALQDGEKQITCRPA
DLLEPEYELMEKEAFAKGIVSKEEDILTYALYPQVAVKFLRGELKAETIPQEKDVSKFMEIPNEYIVEVDGDSFNVKVEP
VYSSGIKKEEKKEIITAETEGAVTSPFRGIVTKIKVSTGASVKQGDQILVLEAMKMENPVECPVDGTVEKIIVKEGQSVN
VGDILMIIK

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=620, Percent_Identity=32.9032258064516, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI106049295, Length=620, Percent_Identity=32.9032258064516, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI106049292, Length=620, Percent_Identity=32.9032258064516, Blast_Score=297, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17562816, Length=621, Percent_Identity=33.8164251207729, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6319695, Length=619, Percent_Identity=32.794830371567, Blast_Score=326, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6321376, Length=619, Percent_Identity=31.5024232633279, Blast_Score=317, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24652212, Length=617, Percent_Identity=35.3322528363047, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24652210, Length=617, Percent_Identity=35.3322528363047, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24652214, Length=617, Percent_Identity=35.3322528363047, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI19921944, Length=617, Percent_Identity=35.3322528363047, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI24652216, Length=617, Percent_Identity=35.3322528363047, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI281363050, Length=617, Percent_Identity=35.3322528363047, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652224, Length=617, Percent_Identity=35.3322528363047, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652222, Length=617, Percent_Identity=35.3322528363047, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652220, Length=617, Percent_Identity=35.3322528363047, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24652218, Length=617, Percent_Identity=35.3322528363047, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 63695; Mature: 63695

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKITDTSFRDAHQSLMATRLRTEDMLPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDP
CEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
WERLRELKGKIQNTPLQMLLRGQNLVGYRHYPDDVVDKFIQKSIENGIDIVRIFDALNDI
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RNVEYSIKAVKKYGAEVQGAISYTTSPVHTTEQFVELAKQFEELGCDSLCIKDMAGLLRP
HHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
FDAKELVGMLKEELSIPIDLHSHCTAGIAPLAYMSSVEAGIDILNCAMSPLSMGTSQPPV
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCH
ESIVAALKGTKYDTNLDLVLLTEIRDYFDEIRNKYKCLINPISERVDARILRYQVPGGML
HHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHH
SNLVSQLKEQGALDKFEEVLNEIPNVRKDLGYPPLVTPTSQIVGTQAVMNVLTDERYKII
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
TNEVANYLKGYYGKSPAPISKDLVKRALQDGEKQITCRPADLLEPEYELMEKEAFAKGIV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHCCCHHHHHHHHHHHHHCC
SKEEDILTYALYPQVAVKFLRGELKAETIPQEKDVSKFMEIPNEYIVEVDGDSFNVKVEP
CCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEEE
VYSSGIKKEEKKEIITAETEGAVTSPFRGIVTKIKVSTGASVKQGDQILVLEAMKMENPV
HHCCCCCHHHHHHHEEECCCCCCCCCHHHHHHEEEECCCCCCCCCCEEEEEEHHHCCCCC
ECPVDGTVEKIIVKEGQSVNVGDILMIIK
CCCCCCHHHHHHHHCCCCCCCCCEEEEEC
>Mature Secondary Structure
MVKITDTSFRDAHQSLMATRLRTEDMLPIAEKMDEVGFYSMEVWGGATFDSCIRYLNEDP
CEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
WERLRELKGKIQNTPLQMLLRGQNLVGYRHYPDDVVDKFIQKSIENGIDIVRIFDALNDI
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RNVEYSIKAVKKYGAEVQGAISYTTSPVHTTEQFVELAKQFEELGCDSLCIKDMAGLLRP
HHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
FDAKELVGMLKEELSIPIDLHSHCTAGIAPLAYMSSVEAGIDILNCAMSPLSMGTSQPPV
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCH
ESIVAALKGTKYDTNLDLVLLTEIRDYFDEIRNKYKCLINPISERVDARILRYQVPGGML
HHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHH
SNLVSQLKEQGALDKFEEVLNEIPNVRKDLGYPPLVTPTSQIVGTQAVMNVLTDERYKII
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
TNEVANYLKGYYGKSPAPISKDLVKRALQDGEKQITCRPADLLEPEYELMEKEAFAKGIV
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEECCHHHCCCHHHHHHHHHHHHHCC
SKEEDILTYALYPQVAVKFLRGELKAETIPQEKDVSKFMEIPNEYIVEVDGDSFNVKVEP
CCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEECCCEEEEEEEE
VYSSGIKKEEKKEIITAETEGAVTSPFRGIVTKIKVSTGASVKQGDQILVLEAMKMENPV
HHCCCCCHHHHHHHEEECCCCCCCCCHHHHHHEEEECCCCCCCCCCEEEEEEHHHCCCCC
ECPVDGTVEKIIVKEGQSVNVGDILMIIK
CCCCCCHHHHHHHHCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]