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The map label for this gene is hemL [H]

Identifier: 45357787

GI number: 45357787

Start: 231125

End: 232408

Strand: Direct

Name: hemL [H]

Synonym: MMP0224

Alternate gene names: 45357787

Gene position: 231125-232408 (Clockwise)

Preceding gene: 45357786

Following gene: 45357791

Centisome position: 13.91

GC content: 34.89

Gene sequence:

>1284_bases
GTGGAATTGAACATCAAAATGGATAGATCAAAAGAACTATTTGAAGAATCAAAAAAATACCTTGTTGGAGGGGTTAACAG
CCCGGTAAGGTTATTTAAGCCATTTCCATTTTTTGTAAAATCTGCAAAAGACTGCTTTTTGTACGACGAAGACGGAAACG
AATTTATTGATTACTGTTTAGCATACGGGCCAATGGTTCTCGGCCATGCAAACGAAAATATCCTAAACGCCGTAAAATCA
CAGATGGATTTAGGAACTGCTTACGGAGTTCCAAGCGAAAAAGAAATTACTCTCGCAAAAGAAGTAATAAATAGGATTCC
TTGCGCAGAAATGGTTAGATTTGTAAATTCAGGAACAGAAGCTACCATGGGTGCAATAAGGCTTGCAAGGGGAGTTACTA
AAAGAAATAAAATCATCAAGTTTGAAGGTGCATTCCACGGTGCTCACGACTACGTGCTTGTAAAAACAGGTAGTGGTGCT
TTAACTCACGGTGCTCCAAATTCCCCTGGAATTCCAGAAGATACTACGAAAAACACGCTTTTAATTCCATTCAATGACGA
AGAAGCAGTAAGAAAAGTAATTTCTGAAAACAAAGAAGAAATTGCATGTATCATCCTTGAACCGGTAATGGGAAATGTCG
GTTGCATTCCTCCAAAAGATGGATATTTACAATTTTTAAGAGAAATTACCGAAGAAAATGGAATACTTTTAATATTTGAT
GAAGTTATTACTGGTTTCAGACTTTCAAAAGGTGGGGCTCAGGAATATTACGGAATAAAATCAGATCTTGCAACTGTTGG
TAAAATTTTGGGCGGAGGATTTCCAATTGGTGCAATAACGGGTAAAAAAGAATATATGGAACAGTTTTCACCAAATGGCC
AGATTTATCAGGCAGGTACATTTAACGGAAACCCGATTTCAGTTACCGCAGGAATTGAAACACTTAAAAATCTTGATGAT
AAATTCTACAAAGAAACAACTAAAAAAGCAGGCATTTTATCAAATTGTTTGAGAGAAACTGCTGAAAAATATAATATTCC
TGCAAAAGTTTACAATGTAGCGTCAATATTCCAAGTTTACTTTAATGATAAAGAAATTGTAACTTATGAAGATGCAAAAT
CAAGCGACACTGAAAAATTCATGAAATATTTCTACACGCTTTTAGAAAACGGTGTATTTGTAGCTCCATCCCAATTTGAA
TGCTGTTTTACATCGATAAAACACAATGATGAAGTTTTAGAAAAAACAATGAATGCAATCGATATTGCAATGAAAAAATT
GTAA

Upstream 100 bases:

>100_bases
CCAGAAAGAATGGATGGGAAGTGGAGTGGTTCCATTTTTCTTCATCTGGTTGGTATTCTGGATAATGACATACAACGGAG
TCTTTTACTAAGGTGGTTTT

Downstream 100 bases:

>100_bases
ATTTTCTTCTTTTTTAATTTTTAAAAATAGCAAATAATTAAAATAAATTAAATGATTTCAGATTTTTCAAGAATTTCTCG
TGCTTTTTCTTTCGAAATTC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MELNIKMDRSKELFEESKKYLVGGVNSPVRLFKPFPFFVKSAKDCFLYDEDGNEFIDYCLAYGPMVLGHANENILNAVKS
QMDLGTAYGVPSEKEITLAKEVINRIPCAEMVRFVNSGTEATMGAIRLARGVTKRNKIIKFEGAFHGAHDYVLVKTGSGA
LTHGAPNSPGIPEDTTKNTLLIPFNDEEAVRKVISENKEEIACIILEPVMGNVGCIPPKDGYLQFLREITEENGILLIFD
EVITGFRLSKGGAQEYYGIKSDLATVGKILGGGFPIGAITGKKEYMEQFSPNGQIYQAGTFNGNPISVTAGIETLKNLDD
KFYKETTKKAGILSNCLRETAEKYNIPAKVYNVASIFQVYFNDKEIVTYEDAKSSDTEKFMKYFYTLLENGVFVAPSQFE
CCFTSIKHNDEVLEKTMNAIDIAMKKL

Sequences:

>Translated_427_residues
MELNIKMDRSKELFEESKKYLVGGVNSPVRLFKPFPFFVKSAKDCFLYDEDGNEFIDYCLAYGPMVLGHANENILNAVKS
QMDLGTAYGVPSEKEITLAKEVINRIPCAEMVRFVNSGTEATMGAIRLARGVTKRNKIIKFEGAFHGAHDYVLVKTGSGA
LTHGAPNSPGIPEDTTKNTLLIPFNDEEAVRKVISENKEEIACIILEPVMGNVGCIPPKDGYLQFLREITEENGILLIFD
EVITGFRLSKGGAQEYYGIKSDLATVGKILGGGFPIGAITGKKEYMEQFSPNGQIYQAGTFNGNPISVTAGIETLKNLDD
KFYKETTKKAGILSNCLRETAEKYNIPAKVYNVASIFQVYFNDKEIVTYEDAKSSDTEKFMKYFYTLLENGVFVAPSQFE
CCFTSIKHNDEVLEKTMNAIDIAMKKL
>Mature_427_residues
MELNIKMDRSKELFEESKKYLVGGVNSPVRLFKPFPFFVKSAKDCFLYDEDGNEFIDYCLAYGPMVLGHANENILNAVKS
QMDLGTAYGVPSEKEITLAKEVINRIPCAEMVRFVNSGTEATMGAIRLARGVTKRNKIIKFEGAFHGAHDYVLVKTGSGA
LTHGAPNSPGIPEDTTKNTLLIPFNDEEAVRKVISENKEEIACIILEPVMGNVGCIPPKDGYLQFLREITEENGILLIFD
EVITGFRLSKGGAQEYYGIKSDLATVGKILGGGFPIGAITGKKEYMEQFSPNGQIYQAGTFNGNPISVTAGIETLKNLDD
KFYKETTKKAGILSNCLRETAEKYNIPAKVYNVASIFQVYFNDKEIVTYEDAKSSDTEKFMKYFYTLLENGVFVAPSQFE
CCFTSIKHNDEVLEKTMNAIDIAMKKL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=336, Percent_Identity=27.6785714285714, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI37574042, Length=341, Percent_Identity=27.5659824046921, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226442705, Length=341, Percent_Identity=26.3929618768328, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI24119277, Length=395, Percent_Identity=25.3164556962025, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI284507298, Length=295, Percent_Identity=26.1016949152542, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI226442709, Length=305, Percent_Identity=27.8688524590164, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI13994255, Length=333, Percent_Identity=25.8258258258258, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=46.4788732394366, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1789016, Length=351, Percent_Identity=30.4843304843305, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1787560, Length=351, Percent_Identity=31.0541310541311, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788044, Length=294, Percent_Identity=31.6326530612245, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1789759, Length=288, Percent_Identity=30.2083333333333, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693181, Length=333, Percent_Identity=30.9309309309309, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786991, Length=299, Percent_Identity=26.7558528428094, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71992977, Length=384, Percent_Identity=28.6458333333333, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25144271, Length=336, Percent_Identity=25.297619047619, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144274, Length=154, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324432, Length=333, Percent_Identity=29.1291291291291, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323470, Length=344, Percent_Identity=25.5813953488372, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357415, Length=338, Percent_Identity=24.8520710059172, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI161085790, Length=336, Percent_Identity=23.8095238095238, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28574759, Length=336, Percent_Identity=23.8095238095238, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 47427; Mature: 47427

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNIKMDRSKELFEESKKYLVGGVNSPVRLFKPFPFFVKSAKDCFLYDEDGNEFIDYCL
CCEEEECCCCHHHHHHHHHEEECCCCCCHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHH
AYGPMVLGHANENILNAVKSQMDLGTAYGVPSEKEITLAKEVINRIPCAEMVRFVNSGTE
HHCCEEEECCCHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCH
ATMGAIRLARGVTKRNKIIKFEGAFHGAHDYVLVKTGSGALTHGAPNSPGIPEDTTKNTL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEE
LIPFNDEEAVRKVISENKEEIACIILEPVMGNVGCIPPKDGYLQFLREITEENGILLIFD
EEEECCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEH
EVITGFRLSKGGAQEYYGIKSDLATVGKILGGGFPIGAITGKKEYMEQFSPNGQIYQAGT
HHHCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCCEEEECC
FNGNPISVTAGIETLKNLDDKFYKETTKKAGILSNCLRETAEKYNIPAKVYNVASIFQVY
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FNDKEIVTYEDAKSSDTEKFMKYFYTLLENGVFVAPSQFECCFTSIKHNDEVLEKTMNAI
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHCCCHHHHHHHHHHH
DIAMKKL
HHHHHCC
>Mature Secondary Structure
MELNIKMDRSKELFEESKKYLVGGVNSPVRLFKPFPFFVKSAKDCFLYDEDGNEFIDYCL
CCEEEECCCCHHHHHHHHHEEECCCCCCHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHH
AYGPMVLGHANENILNAVKSQMDLGTAYGVPSEKEITLAKEVINRIPCAEMVRFVNSGTE
HHCCEEEECCCHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCH
ATMGAIRLARGVTKRNKIIKFEGAFHGAHDYVLVKTGSGALTHGAPNSPGIPEDTTKNTL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEE
LIPFNDEEAVRKVISENKEEIACIILEPVMGNVGCIPPKDGYLQFLREITEENGILLIFD
EEEECCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEH
EVITGFRLSKGGAQEYYGIKSDLATVGKILGGGFPIGAITGKKEYMEQFSPNGQIYQAGT
HHHCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHCCCCCEEEECC
FNGNPISVTAGIETLKNLDDKFYKETTKKAGILSNCLRETAEKYNIPAKVYNVASIFQVY
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FNDKEIVTYEDAKSSDTEKFMKYFYTLLENGVFVAPSQFECCFTSIKHNDEVLEKTMNAI
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHHCCCHHHHHHHHHHH
DIAMKKL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA