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The map label for this gene is nfsA [C]

Identifier: 45357678

GI number: 45357678

Start: 123500

End: 124021

Strand: Direct

Name: nfsA [C]

Synonym: MMP0115

Alternate gene names: 45357678

Gene position: 123500-124021 (Clockwise)

Preceding gene: 45357673

Following gene: 45357679

Centisome position: 7.43

GC content: 39.85

Gene sequence:

>522_bases
ATGAATGCAATATTTGAAAGAAGGAGTATTCGACACTACACTTCTGAAGATGTTTCAGAAGAAATAGTCGACGATCTTTT
AAAAGCAGCAATGTCTGCACCGAGTGCATGCGACCAGAGACCATGGGATTTTGTAGTAATTCGTGATAAAAACACACTTT
CAGGAATTTCTAAAATAAACAGGCACGCAAGGATGTTAAACGAAGCACCTGTTTCAATAGTTGTATGCGGTAACATGGAT
CGGGTAAATGGATCATGTGGGCAGTTCTGGGTTCAGGACTGTGCTGCTGCAACTGAAAATATTTTAATAGAAGCTCAGGA
TCGGGGAATTGGTGCAGTATGGCTTGGATTTTATCCTGTAGATGAAAGAGTCGAAAAAATGAGAAAAGTTCTGCATGCGC
CTGAAAATGTAGTTCCTTTTTCAGTTGTGGCGTTAGGAAATCCTGCGGAAAAGCCAGTTCCTGTAGAAAAATTCGATAGA
GAAAGAATACACTATGAAAAATGGCCGACAGGATATCTTTAA

Upstream 100 bases:

>100_bases
GGTTAGCAGTAAAATATATGTTTTTGAAAGATTGATATTTTATATGTAGAAACCAAATTTGGGGGTTACATAACATTTAA
ACGAAAAAGAGGGGGAATAT

Downstream 100 bases:

>100_bases
AAATCTTTTTTAAAACTTTTAAATTTTTAAACTTATGGGGGTGGTTATTTTAAAGATAATCATTACTTTTTTATCCCATC
AAGTTTTAATTTATACAGCA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MNAIFERRSIRHYTSEDVSEEIVDDLLKAAMSAPSACDQRPWDFVVIRDKNTLSGISKINRHARMLNEAPVSIVVCGNMD
RVNGSCGQFWVQDCAAATENILIEAQDRGIGAVWLGFYPVDERVEKMRKVLHAPENVVPFSVVALGNPAEKPVPVEKFDR
ERIHYEKWPTGYL

Sequences:

>Translated_173_residues
MNAIFERRSIRHYTSEDVSEEIVDDLLKAAMSAPSACDQRPWDFVVIRDKNTLSGISKINRHARMLNEAPVSIVVCGNMD
RVNGSCGQFWVQDCAAATENILIEAQDRGIGAVWLGFYPVDERVEKMRKVLHAPENVVPFSVVALGNPAEKPVPVEKFDR
ERIHYEKWPTGYL
>Mature_173_residues
MNAIFERRSIRHYTSEDVSEEIVDDLLKAAMSAPSACDQRPWDFVVIRDKNTLSGISKINRHARMLNEAPVSIVVCGNMD
RVNGSCGQFWVQDCAAATENILIEAQDRGIGAVWLGFYPVDERVEKMRKVLHAPENVVPFSVVALGNPAEKPVPVEKFDR
ERIHYEKWPTGYL

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 19558; Mature: 19558

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIFERRSIRHYTSEDVSEEIVDDLLKAAMSAPSACDQRPWDFVVIRDKNTLSGISKIN
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHH
RHARMLNEAPVSIVVCGNMDRVNGSCGQFWVQDCAAATENILIEAQDRGIGAVWLGFYPV
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECH
DERVEKMRKVLHAPENVVPFSVVALGNPAEKPVPVEKFDRERIHYEKWPTGYL
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNAIFERRSIRHYTSEDVSEEIVDDLLKAAMSAPSACDQRPWDFVVIRDKNTLSGISKIN
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHH
RHARMLNEAPVSIVVCGNMDRVNGSCGQFWVQDCAAATENILIEAQDRGIGAVWLGFYPV
HHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECH
DERVEKMRKVLHAPENVVPFSVVALGNPAEKPVPVEKFDRERIHYEKWPTGYL
HHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]