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The map label for this gene is nifA [H]

Identifier: 42523371

GI number: 42523371

Start: 1813126

End: 1814718

Strand: Direct

Name: nifA [H]

Synonym: Bd1891

Alternate gene names: 42523371

Gene position: 1813126-1814718 (Clockwise)

Preceding gene: 42523367

Following gene: 42523372

Centisome position: 47.93

GC content: 50.66

Gene sequence:

>1593_bases
GTGGAAAGTGTTGTTGGCAGCCTACTTTGGTTGGCAGGATCTGCCGATAACAAGAACATGTCAAAATCACATGAGCTTCT
TAAGTTTATAGGGGACTCCGCGCAAATTTTCTTTGAACAACAGGCAGGGGAAAGTCTCATTTCGCGAATTTTGGATCTGT
GTATTCAGGCCACCGGCGCCGATCGCGGGACGATCTTCTTTGACTCGCAAATAAACCTGGAACAGCACTTCAAATACCTG
AATAGTTATATTGCCACCGGCATCCGCAATCAGACGATCGTGATCAACACAGATCAGGGTATTGCCGGGAAGGTGTTCCG
TACGCGCAAAAGTTATTTCTCAAACTGTGTGGCCCAGGACAAGGATTTCTATCTTGGCGTCGACAGCAAGACCGGATATC
AGACGGAAAAAGTTCTGGCGGTTCCGTTGCACTGGAAAGACGGGAAAGTCATCGGTGTGATCGAGGTTCTGAATAAGGCC
GATGGTGACTTTGATGAAACCGACCAGCACGCCTTGGAAGCTTTATCCATGCTGGCGGCGGTGGCTTTTGAAAACTATGA
AGTCCATCAGAAGAACGAAGAATCCGAATCGGCCATTGAACACCGCAAGAACATCTGGCGCAAAAGTATTTCTTCTGTCA
GCTTGCACTCCAAAGCTCCCGAATTGCAAAAGATCTATGATCACATCGGGGACTTCGGTAAGTCGGACTCAAATATCCTG
ATCACCGGGGAAAGTGGTGTCGGTAAAGAGGTGATCGCAAGACTTGCGCATCACCATTCCAGTCGTCGGGACGGACCTTT
TATCGCCATCAACTGCGCGGCGATCCCAGAGTCCCTGTTTGAGGCCGAACTTTTTGGTGTGGTCAAAGGGGCGGCGACGG
GGACCGTGGCTCGCCAGGGGCAGATTGAGCTGGCGCACCGGGGAACTTTGTTCCTGGATGAAATCGGCGAAATGCCGGTG
GAGATGCAGGCCAAGCTCTTGCGGGTTTTGCAGGAAAGAAAAGTGCAAAGGCTTGGTTCCACTGAGTCACCGAAGGCTGT
GGACTTCCGTTTGATCTGCGCGACCAACAAACATATTGAAGCGCAAATTCAGCAGGAAAAATTCCGCGAGGATCTTTTCT
ATCGTATCAATGTGGTGAATCTGCAGATCCCGTCGTTGCGTGATCGCAAAGGCGATATTCCAGAGCTGGCTCAGGCCATT
TTGGAACAGCTTCATAACAAGCGTGGTTGGAAGCTCAAAAGAATCAGTCCCGAAGCATTGGACCGCTTGATTGACTATCA
CTGGCCCGGAAACATCCGGGAGCTGGAAAATAAAATTGAAAGTGCCTGCCTGTCTGCGGGGGATCGCGCGGTTTTGTTGC
CGGATGATTTCCAGTTTAAAGCCCGACCACAGTTAAGTGTGGTGCCGGACGCCCAAGAAGATTCACTGAAGCGGTTTGAG
AATTTAAGCTTGCGTGAGGCCCGCGAGGCCTTCGAAGCTGAGTTTGTTCAGCGGGCTATTCAGGGTTCATTGGGAAATAA
ATCTGAAGCGGCCCGCCGCCTGGGTGTTTCCCGTGAAGGTCTCAGAAAGATACTTATTAAGAAACCCGCCTAG

Upstream 100 bases:

>100_bases
CAAAGTTTCATGAACTTAAGTTGCGAATCCCGGGCCTAAGCGTAAGGGCTTGAAAACTTTTAGGTCTTAAAATGGAACGC
CCACAATCGGTGGCTTAAAT

Downstream 100 bases:

>100_bases
ATTCCACCGGCAGTTGGCGCCAACCTGGCAACCACGGTGGGCGTCTTGATTTAAGATTGTAGTGAAATTGTGGACTTAGC
CGTTGGCCGGATCCTTGCCT

Product: NtrC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MESVVGSLLWLAGSADNKNMSKSHELLKFIGDSAQIFFEQQAGESLISRILDLCIQATGADRGTIFFDSQINLEQHFKYL
NSYIATGIRNQTIVINTDQGIAGKVFRTRKSYFSNCVAQDKDFYLGVDSKTGYQTEKVLAVPLHWKDGKVIGVIEVLNKA
DGDFDETDQHALEALSMLAAVAFENYEVHQKNEESESAIEHRKNIWRKSISSVSLHSKAPELQKIYDHIGDFGKSDSNIL
ITGESGVGKEVIARLAHHHSSRRDGPFIAINCAAIPESLFEAELFGVVKGAATGTVARQGQIELAHRGTLFLDEIGEMPV
EMQAKLLRVLQERKVQRLGSTESPKAVDFRLICATNKHIEAQIQQEKFREDLFYRINVVNLQIPSLRDRKGDIPELAQAI
LEQLHNKRGWKLKRISPEALDRLIDYHWPGNIRELENKIESACLSAGDRAVLLPDDFQFKARPQLSVVPDAQEDSLKRFE
NLSLREAREAFEAEFVQRAIQGSLGNKSEAARRLGVSREGLRKILIKKPA

Sequences:

>Translated_530_residues
MESVVGSLLWLAGSADNKNMSKSHELLKFIGDSAQIFFEQQAGESLISRILDLCIQATGADRGTIFFDSQINLEQHFKYL
NSYIATGIRNQTIVINTDQGIAGKVFRTRKSYFSNCVAQDKDFYLGVDSKTGYQTEKVLAVPLHWKDGKVIGVIEVLNKA
DGDFDETDQHALEALSMLAAVAFENYEVHQKNEESESAIEHRKNIWRKSISSVSLHSKAPELQKIYDHIGDFGKSDSNIL
ITGESGVGKEVIARLAHHHSSRRDGPFIAINCAAIPESLFEAELFGVVKGAATGTVARQGQIELAHRGTLFLDEIGEMPV
EMQAKLLRVLQERKVQRLGSTESPKAVDFRLICATNKHIEAQIQQEKFREDLFYRINVVNLQIPSLRDRKGDIPELAQAI
LEQLHNKRGWKLKRISPEALDRLIDYHWPGNIRELENKIESACLSAGDRAVLLPDDFQFKARPQLSVVPDAQEDSLKRFE
NLSLREAREAFEAEFVQRAIQGSLGNKSEAARRLGVSREGLRKILIKKPA
>Mature_530_residues
MESVVGSLLWLAGSADNKNMSKSHELLKFIGDSAQIFFEQQAGESLISRILDLCIQATGADRGTIFFDSQINLEQHFKYL
NSYIATGIRNQTIVINTDQGIAGKVFRTRKSYFSNCVAQDKDFYLGVDSKTGYQTEKVLAVPLHWKDGKVIGVIEVLNKA
DGDFDETDQHALEALSMLAAVAFENYEVHQKNEESESAIEHRKNIWRKSISSVSLHSKAPELQKIYDHIGDFGKSDSNIL
ITGESGVGKEVIARLAHHHSSRRDGPFIAINCAAIPESLFEAELFGVVKGAATGTVARQGQIELAHRGTLFLDEIGEMPV
EMQAKLLRVLQERKVQRLGSTESPKAVDFRLICATNKHIEAQIQQEKFREDLFYRINVVNLQIPSLRDRKGDIPELAQAI
LEQLHNKRGWKLKRISPEALDRLIDYHWPGNIRELENKIESACLSAGDRAVLLPDDFQFKARPQLSVVPDAQEDSLKRFE
NLSLREAREAFEAEFVQRAIQGSLGNKSEAARRLGVSREGLRKILIKKPA

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=308, Percent_Identity=43.1818181818182, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790437, Length=335, Percent_Identity=39.7014925373134, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI87082117, Length=400, Percent_Identity=36.25, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1790299, Length=322, Percent_Identity=40.9937888198758, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788905, Length=337, Percent_Identity=39.7626112759644, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1789233, Length=307, Percent_Identity=42.671009771987, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1789087, Length=402, Percent_Identity=35.5721393034826, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI87082152, Length=327, Percent_Identity=37.3088685015291, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786524, Length=336, Percent_Identity=38.3928571428571, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=36.7924528301887, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1787583, Length=362, Percent_Identity=32.3204419889503, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87081858, Length=305, Percent_Identity=31.1475409836066, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789828, Length=245, Percent_Identity=33.0612244897959, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI193202333, Length=225, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 59457; Mature: 59457

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESVVGSLLWLAGSADNKNMSKSHELLKFIGDSAQIFFEQQAGESLISRILDLCIQATGA
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHHHHHHCCC
DRGTIFFDSQINLEQHFKYLNSYIATGIRNQTIVINTDQGIAGKVFRTRKSYFSNCVAQD
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCC
KDFYLGVDSKTGYQTEKVLAVPLHWKDGKVIGVIEVLNKADGDFDETDQHALEALSMLAA
CCEEEECCCCCCCCCCEEEEEEEEECCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VAFENYEVHQKNEESESAIEHRKNIWRKSISSVSLHSKAPELQKIYDHIGDFGKSDSNIL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEE
ITGESGVGKEVIARLAHHHSSRRDGPFIAINCAAIPESLFEAELFGVVKGAATGTVARQG
EECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCEECCC
QIELAHRGTLFLDEIGEMPVEMQAKLLRVLQERKVQRLGSTESPKAVDFRLICATNKHIE
CEEEEECCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHH
AQIQQEKFREDLFYRINVVNLQIPSLRDRKGDIPELAQAILEQLHNKRGWKLKRISPEAL
HHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHH
DRLIDYHWPGNIRELENKIESACLSAGDRAVLLPDDFQFKARPQLSVVPDAQEDSLKRFE
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEECCCCCHHHHHHHH
NLSLREAREAFEAEFVQRAIQGSLGNKSEAARRLGVSREGLRKILIKKPA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MESVVGSLLWLAGSADNKNMSKSHELLKFIGDSAQIFFEQQAGESLISRILDLCIQATGA
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHHHHHHHCCC
DRGTIFFDSQINLEQHFKYLNSYIATGIRNQTIVINTDQGIAGKVFRTRKSYFSNCVAQD
CCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCC
KDFYLGVDSKTGYQTEKVLAVPLHWKDGKVIGVIEVLNKADGDFDETDQHALEALSMLAA
CCEEEECCCCCCCCCCEEEEEEEEECCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VAFENYEVHQKNEESESAIEHRKNIWRKSISSVSLHSKAPELQKIYDHIGDFGKSDSNIL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEE
ITGESGVGKEVIARLAHHHSSRRDGPFIAINCAAIPESLFEAELFGVVKGAATGTVARQG
EECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCEECCC
QIELAHRGTLFLDEIGEMPVEMQAKLLRVLQERKVQRLGSTESPKAVDFRLICATNKHIE
CEEEEECCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHH
AQIQQEKFREDLFYRINVVNLQIPSLRDRKGDIPELAQAILEQLHNKRGWKLKRISPEAL
HHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHH
DRLIDYHWPGNIRELENKIESACLSAGDRAVLLPDDFQFKARPQLSVVPDAQEDSLKRFE
HHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEECCCCCHHHHHHHH
NLSLREAREAFEAEFVQRAIQGSLGNKSEAARRLGVSREGLRKILIKKPA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]