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The map label for this gene is hisH

Identifier: 42523188

GI number: 42523188

Start: 1627986

End: 1628600

Strand: Direct

Name: hisH

Synonym: Bd1691

Alternate gene names: 42523188

Gene position: 1627986-1628600 (Clockwise)

Preceding gene: 42523187

Following gene: 42523189

Centisome position: 43.03

GC content: 44.23

Gene sequence:

>615_bases
ATGATCAAAATTGTCGATTATGGTCTTGGTAATATTCAGGCCTTTGCCAATTTGTATAAAAGACTCCATATTCCGGTTGT
CGTTGCCAGAACCGCTGATGAGCTTGCTGGCGCCACAAAGATTATTCTTCCGGGGGTTGGGGCTTTTGATCATGCAATGG
CAAGATTAAATGAATCAGGAATGCGTGAAATACTGGAAAGCTTGGTTCAAAAAGACAAAGTACCGGTGTTAGGAATTTGC
GTCGGAATGCAAATGCTTGCTAACTCCAGCGACGAGGGTGTCTTGCCGGGTTTAGGTTGGGTCCCAGGTCGTGTTAGAGA
ATTTCGGAATATTCCAGGTTTAGAGGGGATCGCCCAACCTCATATGGGTTGGAACGATGTTAAGCCTGTTGTTAGCTCGG
GTCTTTTTAAGGGACTGGAGACTGATGCTCGTTTCTATTTTCTTCATTCGTTCTTTTTTGAATGCCAAAATCCGGATTAC
TCTCTAGCTAGCGCAAACTATGGTTTGGAATTCAGCTGTGCAGTGGCTAATCGCAACGTCTATGGTGTTCAATTTCACCC
TGAAAAAAGTCACGACTTCGGAATGACGCTGTTGAAGAACTTTTCGGAGATTTAG

Upstream 100 bases:

>100_bases
GCAAGAATAGAACGTGTCTTGACTATAAGAATAAACGGTTTGTCATTGGGCTTGGGTCCAGAGTTATGAGTGCTTTGGGG
TTGGAAAGAAGACTATTCCG

Downstream 100 bases:

>100_bases
TATGTTAAGACCACGTATAATTCCGTGCCTCCTAGTTAAGGATGGCGGTCTAGTTAAAACGGTAAAATTCAAGGATCCTA
AATATGTGGGCGATCCGATT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIKIVDYGLGNIQAFANLYKRLHIPVVVARTADELAGATKIILPGVGAFDHAMARLNESGMREILESLVQKDKVPVLGIC
VGMQMLANSSDEGVLPGLGWVPGRVREFRNIPGLEGIAQPHMGWNDVKPVVSSGLFKGLETDARFYFLHSFFFECQNPDY
SLASANYGLEFSCAVANRNVYGVQFHPEKSHDFGMTLLKNFSEI

Sequences:

>Translated_204_residues
MIKIVDYGLGNIQAFANLYKRLHIPVVVARTADELAGATKIILPGVGAFDHAMARLNESGMREILESLVQKDKVPVLGIC
VGMQMLANSSDEGVLPGLGWVPGRVREFRNIPGLEGIAQPHMGWNDVKPVVSSGLFKGLETDARFYFLHSFFFECQNPDY
SLASANYGLEFSCAVANRNVYGVQFHPEKSHDFGMTLLKNFSEI
>Mature_204_residues
MIKIVDYGLGNIQAFANLYKRLHIPVVVARTADELAGATKIILPGVGAFDHAMARLNESGMREILESLVQKDKVPVLGIC
VGMQMLANSSDEGVLPGLGWVPGRVREFRNIPGLEGIAQPHMGWNDVKPVVSSGLFKGLETDARFYFLHSFFFECQNPDY
SLASANYGLEFSCAVANRNVYGVQFHPEKSHDFGMTLLKNFSEI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=38.7254901960784, Blast_Score=130, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6319725, Length=213, Percent_Identity=31.4553990610329, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BDEBA (P60597)

Other databases:

- EMBL:   BX842650
- RefSeq:   NP_968568.1
- ProteinModelPortal:   P60597
- SMR:   P60597
- GeneID:   2734814
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1691
- NMPDR:   fig|264462.1.peg.1538
- HOGENOM:   HBG292341
- OMA:   ATKIILP
- PhylomeDB:   P60597
- ProtClustDB:   PRK13181
- BioCyc:   BBAC264462:BD1691-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22493; Mature: 22493

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 80-80 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIVDYGLGNIQAFANLYKRLHIPVVVARTADELAGATKIILPGVGAFDHAMARLNESG
CEEEEECCCCHHHHHHHHHHHHCCCEEEEECHHHHCCCEEEEECCCCHHHHHHHHHCHHH
MREILESLVQKDKVPVLGICVGMQMLANSSDEGVLPGLGWVPGRVREFRNIPGLEGIAQP
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
HMGWNDVKPVVSSGLFKGLETDARFYFLHSFFFECQNPDYSLASANYGLEFSCAVANRNV
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCE
YGVQFHPEKSHDFGMTLLKNFSEI
EEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKIVDYGLGNIQAFANLYKRLHIPVVVARTADELAGATKIILPGVGAFDHAMARLNESG
CEEEEECCCCHHHHHHHHHHHHCCCEEEEECHHHHCCCEEEEECCCCHHHHHHHHHCHHH
MREILESLVQKDKVPVLGICVGMQMLANSSDEGVLPGLGWVPGRVREFRNIPGLEGIAQP
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
HMGWNDVKPVVSSGLFKGLETDARFYFLHSFFFECQNPDYSLASANYGLEFSCAVANRNV
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCE
YGVQFHPEKSHDFGMTLLKNFSEI
EEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA