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The map label for this gene is hisF [H]

Identifier: 42523189

GI number: 42523189

Start: 1628602

End: 1629381

Strand: Direct

Name: hisF [H]

Synonym: Bd1692

Alternate gene names: 42523189

Gene position: 1628602-1629381 (Clockwise)

Preceding gene: 42523188

Following gene: 42523190

Centisome position: 43.05

GC content: 45.0

Gene sequence:

>780_bases
ATGTTAAGACCACGTATAATTCCGTGCCTCCTAGTTAAGGATGGCGGTCTAGTTAAAACGGTAAAATTCAAGGATCCTAA
ATATGTGGGCGATCCGATTAATGCTGTTAAAATATTTAATGAAAAGCGTGCTGATGAGCTGATTGTTGTGGACATTGATG
CAACAGTTAATGGCGTCGAGCCAAATTATAAAATGATCAGTAATCTGGCTATGGAATGCCGTATGCCGCTATGTTATGGC
GGTGGAGTTCGAACAGCCGCTCAAGCGAAGAAGATTGTGTCATTGGGAGTAGAGAAGGTCGCAATCAGTGCGGCGGCTCT
TGAAAATATGAGTCTTATCACTCAGATGGCCGAGGAAATCGGACGCCAGAGTGTTGTTGTCGTCTTAGACGTAAAAAAGC
GGTTGTTAGGGCGTACATTTGATGTTTGGACTCACAATGGCACTAAGAATACAAAGCGCGAGTTTATTGAGGTAGCTAAA
GAGGCAGAGCAGCTTGGAGCGGGAGAGATAGTCGTTAACTCAATTGACGACGATGGTAAAATGTCTGGCTACGATGTCGC
TCTTATTAAAAAGCTGCGTGAGTCGGTGAAAATTCCGGTTACAGTTCTTGGGGGGGCGGGTTCCCTCGATGATATTTCTA
AAGTTATTGAGGAATGCGGTACTGTTGGCGTGGCTGCAGGAAGTTTGTTTGTCTTCAAGGGGCCCTATAAGGCTGTTCTG
ATCAATTACCCGGATTTGCGTCAGAAGGATGAAATGATCAAAAGCGCATTGCGCAGGTAA

Upstream 100 bases:

>100_bases
TGTGCAGTGGCTAATCGCAACGTCTATGGTGTTCAATTTCACCCTGAAAAAAGTCACGACTTCGGAATGACGCTGTTGAA
GAACTTTTCGGAGATTTAGT

Downstream 100 bases:

>100_bases
TGAGAGATTAATATGGCTTCGTATTTTAACGGTAAAACATTGTTGATCACTGGCGGAACAGGCTCTTTTGGGAATGCTGT
TCTTAATAGATTCTTAAAGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF3; ImGP synthase subunit hisF3; IGPS subunit hisF3 [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLRPRIIPCLLVKDGGLVKTVKFKDPKYVGDPINAVKIFNEKRADELIVVDIDATVNGVEPNYKMISNLAMECRMPLCYG
GGVRTAAQAKKIVSLGVEKVAISAAALENMSLITQMAEEIGRQSVVVVLDVKKRLLGRTFDVWTHNGTKNTKREFIEVAK
EAEQLGAGEIVVNSIDDDGKMSGYDVALIKKLRESVKIPVTVLGGAGSLDDISKVIEECGTVGVAAGSLFVFKGPYKAVL
INYPDLRQKDEMIKSALRR

Sequences:

>Translated_259_residues
MLRPRIIPCLLVKDGGLVKTVKFKDPKYVGDPINAVKIFNEKRADELIVVDIDATVNGVEPNYKMISNLAMECRMPLCYG
GGVRTAAQAKKIVSLGVEKVAISAAALENMSLITQMAEEIGRQSVVVVLDVKKRLLGRTFDVWTHNGTKNTKREFIEVAK
EAEQLGAGEIVVNSIDDDGKMSGYDVALIKKLRESVKIPVTVLGGAGSLDDISKVIEECGTVGVAAGSLFVFKGPYKAVL
INYPDLRQKDEMIKSALRR
>Mature_259_residues
MLRPRIIPCLLVKDGGLVKTVKFKDPKYVGDPINAVKIFNEKRADELIVVDIDATVNGVEPNYKMISNLAMECRMPLCYG
GGVRTAAQAKKIVSLGVEKVAISAAALENMSLITQMAEEIGRQSVVVVLDVKKRLLGRTFDVWTHNGTKNTKREFIEVAK
EAEQLGAGEIVVNSIDDDGKMSGYDVALIKKLRESVKIPVTVLGGAGSLDDISKVIEECGTVGVAAGSLFVFKGPYKAVL
INYPDLRQKDEMIKSALRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=234, Percent_Identity=35.042735042735, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI87082028, Length=226, Percent_Identity=23.0088495575221, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28222; Mature: 28222

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPRIIPCLLVKDGGLVKTVKFKDPKYVGDPINAVKIFNEKRADELIVVDIDATVNGVE
CCCCCCEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEEECCEECCCC
PNYKMISNLAMECRMPLCYGGGVRTAAQAKKIVSLGVEKVAISAAALENMSLITQMAEEI
CCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRQSVVVVLDVKKRLLGRTFDVWTHNGTKNTKREFIEVAKEAEQLGAGEIVVNSIDDDGK
CCCEEEEEEECHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
MSGYDVALIKKLRESVKIPVTVLGGAGSLDDISKVIEECGTVGVAAGSLFVFKGPYKAVL
CCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEECCEEEEEECCCEEEE
INYPDLRQKDEMIKSALRR
ECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLRPRIIPCLLVKDGGLVKTVKFKDPKYVGDPINAVKIFNEKRADELIVVDIDATVNGVE
CCCCCCEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEEECCEECCCC
PNYKMISNLAMECRMPLCYGGGVRTAAQAKKIVSLGVEKVAISAAALENMSLITQMAEEI
CCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRQSVVVVLDVKKRLLGRTFDVWTHNGTKNTKREFIEVAKEAEQLGAGEIVVNSIDDDGK
CCCEEEEEEECHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
MSGYDVALIKKLRESVKIPVTVLGGAGSLDDISKVIEECGTVGVAAGSLFVFKGPYKAVL
CCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEECCEEEEEECCCEEEE
INYPDLRQKDEMIKSALRR
ECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA