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The map label for this gene is pilB [H]

Identifier: 42523016

GI number: 42523016

Start: 1429968

End: 1431668

Strand: Direct

Name: pilB [H]

Synonym: Bd1509

Alternate gene names: 42523016

Gene position: 1429968-1431668 (Clockwise)

Preceding gene: 42523015

Following gene: 42523017

Centisome position: 37.8

GC content: 50.62

Gene sequence:

>1701_bases
ATGTCTTCACTCAAAATCGGGGAAATTCTAGTCAAGCAAGGTTTGCTCAAGCCTGACCAATTGGCGTTGGCCATGGAAGA
GCAGAAAAAAACTGGGCAGAAGCTGACGAATTCCATCATTCAACTCGGGTACTTAAAAGACAATCAGATCCTTCGCGCTG
TTGAAAAACACTTCGCGGTTCCAGGGGTCGAGGTCAACACGTTTGAAATCGATGCAACTGTCATTGCAATGATTCCCAAA
GATGTGTGTGAGAAAAACACATTGATCCCTTTGCAGAAAGCCGGGAACACCCTGGTTGTCGCTTTTGCTGATCCTTCTAA
TATTATAGTCAAAGAAGATTTGCGTTTCATCACTCGTTGCCGCATCCAAGCGGTGGTGGGGACTGAAAGTGCCATCATGT
CCGGTATTGAAAAATACTATGGCGGCAGCATCAGCACCAAGTCTTTGAACACCATGGGGGTGGGTGATGAGGATGACTTT
ATCCTCAGTGCCGGTCCTGCAACAGAAGTCATCGATCAGGACGGTGGGTCTGAAGACGCGCCGATCATCAAGTTCGTGAA
CTCGATCCTGGCCGATGCGATCCGCAAAAAAGTATCGGATATTCACTTTGAACCTTACGAAAAAAAGTACCGCGTGCGTT
TCCGTATCGACGGGAACTTGGTGGAAGCTACAGCACCTCCGGCAGGTACGGCGGCGGCAATTGCCTCTCGTATTAAGATC
ATGTCCAAACTTGATATCGCCGAAAAACGTCGTCCGCAGGATGGTCGTTTGAAAGTGCGCACCAAGGCCAAAGAGATGGA
CTTCCGTGTCAGCGTACTGCCGACTCTTTGGGGTGAAAAAGTTGTTTTGCGTCTTTTGGATAAATCAAACCTGCAGCTGG
ATATGACCAAGCTGGGTTTTGAAGAAGACGATTTGAAACTGTTCAAAGCCACCATCAATTTGCCCCAAGGCATGGTTCTG
ATCACGGGTCCGACGGGTTCGGGTAAAACCACCACGATTTATTCCGCCTTGGCGGAGCTGAATCAGCCGGACGTGAACAT
CTCCACCGCGGAAGATCCGGTGGAGTTCAACTTGGAAGGTATCAACCAGGTTCAGATGAACCCGGATATTGATTTGAATT
TCTCCAGCGCCCTGAAGTCCTTCCTGCGTCAGGATCCGGATGTTGTGATGGTGGGGGAGATTCGTGACCTTGAAACCGCC
GAGATCGCGTTTAAAGCGGCCTCCACAGGTCACTTGGTCGTAAGCACCCTGCATACCAACGATGCACCTGGAACGGTCAT
TCGTCTGACCGAGATGGGTGTGGCTCCGTACATTATCACTTCGACTGTGAATCTGATCGTGGCTCAGCGTCTGGTCGGTA
AAGTCTGCGAGTCCTGCAAAGCGCCTGTGGAAGTGCCGGCGCAAACGCTGCTGAATCTGGGCGTGCCGCAAGCTGAAATC
GGGGACTATAAATTGATGCGAGGAAAAGGTTGCGCCAACTGCAACAACACCGGCATCAAAGGCCGTCTGGCGATTTACGA
GTTGCTGGCCATGACCGAGAAAATGAAAGAGGCCATCTTGAAGGGTGCCTCCACCGGTCAGCTTCGTTTCCTGGCCCGTG
AACAGGGGATGAGAACTCTGCGCCGAAGTGCGCTGTTAAAGTTAAAACGTGGCGTAACAACTATTGAAGAAGTGCTCAAC
GCATCAGTGAAGGACACCTAA

Upstream 100 bases:

>100_bases
ATATTCGCGAAAGCTGGACCCGCTTTTTGAAACAAAACTCATCCTCGGTCTAGTTACAAAAAATGGCACCCTGGAGTAAA
GTGAGTCCAGGGGAGTCCTA

Downstream 100 bases:

>100_bases
TGAGTCTGACCGAACTCCTTTTGCAGGCTAAAGCCAAGAAAGCGGAAGAATTTTTGTTTGTTGTCGGAAGTGAGCCGCGT
GCTCGTCTGGCCTCGGGCTG

Product: type IV pilus biogenesis protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSSLKIGEILVKQGLLKPDQLALAMEEQKKTGQKLTNSIIQLGYLKDNQILRAVEKHFAVPGVEVNTFEIDATVIAMIPK
DVCEKNTLIPLQKAGNTLVVAFADPSNIIVKEDLRFITRCRIQAVVGTESAIMSGIEKYYGGSISTKSLNTMGVGDEDDF
ILSAGPATEVIDQDGGSEDAPIIKFVNSILADAIRKKVSDIHFEPYEKKYRVRFRIDGNLVEATAPPAGTAAAIASRIKI
MSKLDIAEKRRPQDGRLKVRTKAKEMDFRVSVLPTLWGEKVVLRLLDKSNLQLDMTKLGFEEDDLKLFKATINLPQGMVL
ITGPTGSGKTTTIYSALAELNQPDVNISTAEDPVEFNLEGINQVQMNPDIDLNFSSALKSFLRQDPDVVMVGEIRDLETA
EIAFKAASTGHLVVSTLHTNDAPGTVIRLTEMGVAPYIITSTVNLIVAQRLVGKVCESCKAPVEVPAQTLLNLGVPQAEI
GDYKLMRGKGCANCNNTGIKGRLAIYELLAMTEKMKEAILKGASTGQLRFLAREQGMRTLRRSALLKLKRGVTTIEEVLN
ASVKDT

Sequences:

>Translated_566_residues
MSSLKIGEILVKQGLLKPDQLALAMEEQKKTGQKLTNSIIQLGYLKDNQILRAVEKHFAVPGVEVNTFEIDATVIAMIPK
DVCEKNTLIPLQKAGNTLVVAFADPSNIIVKEDLRFITRCRIQAVVGTESAIMSGIEKYYGGSISTKSLNTMGVGDEDDF
ILSAGPATEVIDQDGGSEDAPIIKFVNSILADAIRKKVSDIHFEPYEKKYRVRFRIDGNLVEATAPPAGTAAAIASRIKI
MSKLDIAEKRRPQDGRLKVRTKAKEMDFRVSVLPTLWGEKVVLRLLDKSNLQLDMTKLGFEEDDLKLFKATINLPQGMVL
ITGPTGSGKTTTIYSALAELNQPDVNISTAEDPVEFNLEGINQVQMNPDIDLNFSSALKSFLRQDPDVVMVGEIRDLETA
EIAFKAASTGHLVVSTLHTNDAPGTVIRLTEMGVAPYIITSTVNLIVAQRLVGKVCESCKAPVEVPAQTLLNLGVPQAEI
GDYKLMRGKGCANCNNTGIKGRLAIYELLAMTEKMKEAILKGASTGQLRFLAREQGMRTLRRSALLKLKRGVTTIEEVLN
ASVKDT
>Mature_565_residues
SSLKIGEILVKQGLLKPDQLALAMEEQKKTGQKLTNSIIQLGYLKDNQILRAVEKHFAVPGVEVNTFEIDATVIAMIPKD
VCEKNTLIPLQKAGNTLVVAFADPSNIIVKEDLRFITRCRIQAVVGTESAIMSGIEKYYGGSISTKSLNTMGVGDEDDFI
LSAGPATEVIDQDGGSEDAPIIKFVNSILADAIRKKVSDIHFEPYEKKYRVRFRIDGNLVEATAPPAGTAAAIASRIKIM
SKLDIAEKRRPQDGRLKVRTKAKEMDFRVSVLPTLWGEKVVLRLLDKSNLQLDMTKLGFEEDDLKLFKATINLPQGMVLI
TGPTGSGKTTTIYSALAELNQPDVNISTAEDPVEFNLEGINQVQMNPDIDLNFSSALKSFLRQDPDVVMVGEIRDLETAE
IAFKAASTGHLVVSTLHTNDAPGTVIRLTEMGVAPYIITSTVNLIVAQRLVGKVCESCKAPVEVPAQTLLNLGVPQAEIG
DYKLMRGKGCANCNNTGIKGRLAIYELLAMTEKMKEAILKGASTGQLRFLAREQGMRTLRRSALLKLKRGVTTIEEVLNA
SVKDT

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=404, Percent_Identity=45.5445544554455, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=43.6363636363636, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI87082188, Length=255, Percent_Identity=32.9411764705882, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 61784; Mature: 61652

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLKIGEILVKQGLLKPDQLALAMEEQKKTGQKLTNSIIQLGYLKDNQILRAVEKHFAV
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCC
PGVEVNTFEIDATVIAMIPKDVCEKNTLIPLQKAGNTLVVAFADPSNIIVKEDLRFITRC
CCCEEEEEEECEEEEEECCHHHHCCCCEEEHCCCCCEEEEEEECCCCEEEHHHHHHHHHH
RIQAVVGTESAIMSGIEKYYGGSISTKSLNTMGVGDEDDFILSAGPATEVIDQDGGSEDA
HHHEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCC
PIIKFVNSILADAIRKKVSDIHFEPYEKKYRVRFRIDGNLVEATAPPAGTAAAIASRIKI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHH
MSKLDIAEKRRPQDGRLKVRTKAKEMDFRVSVLPTLWGEKVVLRLLDKSNLQLDMTKLGF
HHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHCCCCCEEEHHHCCC
EEDDLKLFKATINLPQGMVLITGPTGSGKTTTIYSALAELNQPDVNISTAEDPVEFNLEG
CCHHHHHEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEECC
INQVQMNPDIDLNFSSALKSFLRQDPDVVMVGEIRDLETAEIAFKAASTGHLVVSTLHTN
CEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEECCCCCEEEEEEECC
DAPGTVIRLTEMGVAPYIITSTVNLIVAQRLVGKVCESCKAPVEVPAQTLLNLGVPQAEI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCC
GDYKLMRGKGCANCNNTGIKGRLAIYELLAMTEKMKEAILKGASTGQLRFLAREQGMRTL
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
RRSALLKLKRGVTTIEEVLNASVKDT
HHHHHHHHHHCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSLKIGEILVKQGLLKPDQLALAMEEQKKTGQKLTNSIIQLGYLKDNQILRAVEKHFAV
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCC
PGVEVNTFEIDATVIAMIPKDVCEKNTLIPLQKAGNTLVVAFADPSNIIVKEDLRFITRC
CCCEEEEEEECEEEEEECCHHHHCCCCEEEHCCCCCEEEEEEECCCCEEEHHHHHHHHHH
RIQAVVGTESAIMSGIEKYYGGSISTKSLNTMGVGDEDDFILSAGPATEVIDQDGGSEDA
HHHEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCC
PIIKFVNSILADAIRKKVSDIHFEPYEKKYRVRFRIDGNLVEATAPPAGTAAAIASRIKI
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHH
MSKLDIAEKRRPQDGRLKVRTKAKEMDFRVSVLPTLWGEKVVLRLLDKSNLQLDMTKLGF
HHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHCCCCCEEEHHHCCC
EEDDLKLFKATINLPQGMVLITGPTGSGKTTTIYSALAELNQPDVNISTAEDPVEFNLEG
CCHHHHHEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEECC
INQVQMNPDIDLNFSSALKSFLRQDPDVVMVGEIRDLETAEIAFKAASTGHLVVSTLHTN
CEEEECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEECCCCCEEEEEEECC
DAPGTVIRLTEMGVAPYIITSTVNLIVAQRLVGKVCESCKAPVEVPAQTLLNLGVPQAEI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCC
GDYKLMRGKGCANCNNTGIKGRLAIYELLAMTEKMKEAILKGASTGQLRFLAREQGMRTL
CCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
RRSALLKLKRGVTTIEEVLNASVKDT
HHHHHHHHHHCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]