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The map label for this gene is prfB [H]

Identifier: 42522998

GI number: 42522998

Start: 1409428

End: 1410393

Strand: Direct

Name: prfB [H]

Synonym: Bd1489

Alternate gene names: 42522998

Gene position: 1409428-1410393 (Clockwise)

Preceding gene: 42522997

Following gene: 42522999

Centisome position: 37.26

GC content: 50.31

Gene sequence:

>966_bases
ATGCAAAAGCTGAACAAAGAAAAAGCCCTGCTTGAAAAAGCCGTGGGGGAATTCGACGCCTTTGCAAGTCGCTTGAGCGA
CTCCAAGGTTCTGCTGGAAATGGCCGAAGAAGCCCAGGATGAAGGCAGTTTCTCTGAAGCCAAAGCCGAGGTCGTGGCAC
TGGAAAAGTTGGGGGAAGAGCTTGAGTTGAAACGAGTTCTGAACGGCGAATTGGACAGCAACAGCTCGTATCTGTCCATC
AACTCCGGGGCAGGTGGTACGGAGTCCTGTGACTGGGCCTCCATGCTTTTGCGTATGTACACGCGTTATGCTGACAAGCA
TGGCTTTAAAGTTCAAATCATCGAGATGACCGAGGGCGAGGGTGCCGGGATTAAATCCTGCACGCTTTTGATCGAAGGCC
CTTATGCCTATGGATATCTGAAGGCGGAATCCGGAGTGCATCGTCTGGTGCGTATTTCTCCGTTTGATTCCAATGCCCGT
CGCCATACATCTTTTGCGTCCGTGTTTGCCTGGGCGGAAGTGGATGATGACATCAATATCGAAGTTCGTCCTGATGATCT
GAAAGTGGACACCTTCCGCGCCAGCGGGGCCGGTGGCCAGCACGTCAACAGAACGGATTCTGCGGTTCGTATGACTCACA
TTCCATCCGGGGTTATTGTGACCTGTCAGGTGGAAAGATCTCAGATTCAGAACCGTGAAAAAGCCCTGAAGATGTTGAAA
GCGCGCCTTTATGAAATGGAAATCGAAAAGCGCAACGCGGAAAAAGACGCGATGAATTCCCAGAAAAAAGCCAATGAATG
GGGTTCGCAGATTCGCTCTTACGTGATGCATCCTTATCAGATGGTGAAAGATCACCGCACTGATTTTGAAACCAATCAGG
TCGATGACGTGATGGACGGGGATCTGGATGGATTTATCATGTCTTACTTGAAATCGACTTTGACGGCAGAAAGTCAGCCT
TCGTGA

Upstream 100 bases:

>100_bases
GAGCTTCGGGGGTATCTTTGACTTAGATCGCAAGAAAAAGCGCCTGGAAGAGATCTCCATTCAGGCTGAAAATCCCGCAC
TCTGGGAAAAACCTGCTGAA

Downstream 100 bases:

>100_bases
AGTCTTCATTGCCTTGGCTTTCCTGGCGACTGCTTTTCTCGCGCAAAACCATGTTTGGCGGCTCGGCTCCTTTGGCCTTG
CTCGGACTGGTTTTGGGGGT

Product: hypothetical protein

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQKLNKEKALLEKAVGEFDAFASRLSDSKVLLEMAEEAQDEGSFSEAKAEVVALEKLGEELELKRVLNGELDSNSSYLSI
NSGAGGTESCDWASMLLRMYTRYADKHGFKVQIIEMTEGEGAGIKSCTLLIEGPYAYGYLKAESGVHRLVRISPFDSNAR
RHTSFASVFAWAEVDDDINIEVRPDDLKVDTFRASGAGGQHVNRTDSAVRMTHIPSGVIVTCQVERSQIQNREKALKMLK
ARLYEMEIEKRNAEKDAMNSQKKANEWGSQIRSYVMHPYQMVKDHRTDFETNQVDDVMDGDLDGFIMSYLKSTLTAESQP
S

Sequences:

>Translated_321_residues
MQKLNKEKALLEKAVGEFDAFASRLSDSKVLLEMAEEAQDEGSFSEAKAEVVALEKLGEELELKRVLNGELDSNSSYLSI
NSGAGGTESCDWASMLLRMYTRYADKHGFKVQIIEMTEGEGAGIKSCTLLIEGPYAYGYLKAESGVHRLVRISPFDSNAR
RHTSFASVFAWAEVDDDINIEVRPDDLKVDTFRASGAGGQHVNRTDSAVRMTHIPSGVIVTCQVERSQIQNREKALKMLK
ARLYEMEIEKRNAEKDAMNSQKKANEWGSQIRSYVMHPYQMVKDHRTDFETNQVDDVMDGDLDGFIMSYLKSTLTAESQP
S
>Mature_321_residues
MQKLNKEKALLEKAVGEFDAFASRLSDSKVLLEMAEEAQDEGSFSEAKAEVVALEKLGEELELKRVLNGELDSNSSYLSI
NSGAGGTESCDWASMLLRMYTRYADKHGFKVQIIEMTEGEGAGIKSCTLLIEGPYAYGYLKAESGVHRLVRISPFDSNAR
RHTSFASVFAWAEVDDDINIEVRPDDLKVDTFRASGAGGQHVNRTDSAVRMTHIPSGVIVTCQVERSQIQNREKALKMLK
ARLYEMEIEKRNAEKDAMNSQKKANEWGSQIRSYVMHPYQMVKDHRTDFETNQVDDVMDGDLDGFIMSYLKSTLTAESQP
S

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=258, Percent_Identity=37.2093023255814, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI34577120, Length=311, Percent_Identity=33.4405144694534, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI166795305, Length=163, Percent_Identity=38.0368098159509, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI2367172, Length=313, Percent_Identity=55.9105431309904, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=41.0788381742739, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=272, Percent_Identity=31.6176470588235, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=328, Percent_Identity=36.280487804878, Blast_Score=153, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19921226, Length=229, Percent_Identity=38.4279475982533, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35947; Mature: 35947

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLNKEKALLEKAVGEFDAFASRLSDSKVLLEMAEEAQDEGSFSEAKAEVVALEKLGEE
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHH
LELKRVLNGELDSNSSYLSINSGAGGTESCDWASMLLRMYTRYADKHGFKVQIIEMTEGE
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GAGIKSCTLLIEGPYAYGYLKAESGVHRLVRISPFDSNARRHTSFASVFAWAEVDDDINI
CCCCEEEEEEEECCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHEEEECCCCCEE
EVRPDDLKVDTFRASGAGGQHVNRTDSAVRMTHIPSGVIVTCQVERSQIQNREKALKMLK
EECCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHH
ARLYEMEIEKRNAEKDAMNSQKKANEWGSQIRSYVMHPYQMVKDHRTDFETNQVDDVMDG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
DLDGFIMSYLKSTLTAESQPS
CHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQKLNKEKALLEKAVGEFDAFASRLSDSKVLLEMAEEAQDEGSFSEAKAEVVALEKLGEE
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHH
LELKRVLNGELDSNSSYLSINSGAGGTESCDWASMLLRMYTRYADKHGFKVQIIEMTEGE
HHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
GAGIKSCTLLIEGPYAYGYLKAESGVHRLVRISPFDSNARRHTSFASVFAWAEVDDDINI
CCCCEEEEEEEECCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHEEEECCCCCEE
EVRPDDLKVDTFRASGAGGQHVNRTDSAVRMTHIPSGVIVTCQVERSQIQNREKALKMLK
EECCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHHHHHHHHHHHHH
ARLYEMEIEKRNAEKDAMNSQKKANEWGSQIRSYVMHPYQMVKDHRTDFETNQVDDVMDG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCC
DLDGFIMSYLKSTLTAESQPS
CHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA