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The map label for this gene is potB [H]

Identifier: 42522936

GI number: 42522936

Start: 1341484

End: 1342317

Strand: Direct

Name: potB [H]

Synonym: Bd1420

Alternate gene names: 42522936

Gene position: 1341484-1342317 (Clockwise)

Preceding gene: 42522935

Following gene: 42522937

Centisome position: 35.46

GC content: 53.0

Gene sequence:

>834_bases
GTGAAATTACGAGATCTGTTCCTTCTGCCGGTGGGATTGTGGTTTGTGGTCTTTGTCCTGCTGCCACTTATTATCGTCGC
TGTGGTGAGTTTTGCCACGCGGGGAACCTATGGTGGTGTGGAGTGGTCGTGGACCCTTGCCAACTATGTACGGGCTTTTT
CGCCGACCTATCTGGGAATTTTTGCTGAAAGTTTCAAGCTGGCTTTGATCACCACCGGGGTCTGTCTGGTTCTGGGCGTT
CTGGTGTCCTGGGCCATGGCGACGGCATCCACGCGTTTGCGTCAGCTTTATCTGATGGCTGTGGTGCTGCCGTTTTTGAC
CAATCTGGTGATTCGTATCTATGCCATCCGGCTTTTTGTTGGTGCCGACGGTCCGCTGCAGGCGGGGTTGACATTATTGG
GAGTTCCCTTTGATTCCTTCGCGCTGACGCAGAACAAGTGGCTGGTGCTTTACGGAATGGTCACGACTTATCTGCCATTC
ATGGTGCTGCCGTTGTACGGGGCTTTTGAAAAGTTTGATTTTTCCTTGGTGGAAGCGGCTCAGGATCTGGGCGCCGGGAT
CTGGAAGATTCTTTTCACAGTGATTTTACCCAACCTTAAGAAAGCTCTGTGGGCGGGGGCTTTGCTGGTGTTCATTCCTT
CTTTGGGTGAATACGTGATACCGGATCTTCTGGGGGGCGCTAAAAACATGCTTTATGGAAATCTGATCACCGAACAGTTT
TTGAAAGCCCGCGACTGGCCCTTTGGGGCGGCATTGTCGGTGCTGATGATGCTGCTTTTGCTGGTGATGGCGCTGGTGTT
CAGAATGTGGGAGGCGCGCCGTGCCCGTGGTTGA

Upstream 100 bases:

>100_bases
TTATCAAAGGTAAAATCAATGTCCCACCGGATCAGATCCGCCTGGGGATGAGCATTGAAGCCTACGTCCGTCGCGCCATG
GTCTTTGGCGAGCGTGAAAA

Downstream 100 bases:

>100_bases
ACGAAGCCGTCCTCAGGCGCCCATGTTTGCGAAAGTCGTTCTGTGGATAACACTGCTTTTGCTGCACACTCCGATTCTGG
TGATGATTCTGGGCGCTTTG

Product: spermidine/putrescine transport system permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKLRDLFLLPVGLWFVVFVLLPLIIVAVVSFATRGTYGGVEWSWTLANYVRAFSPTYLGIFAESFKLALITTGVCLVLGV
LVSWAMATASTRLRQLYLMAVVLPFLTNLVIRIYAIRLFVGADGPLQAGLTLLGVPFDSFALTQNKWLVLYGMVTTYLPF
MVLPLYGAFEKFDFSLVEAAQDLGAGIWKILFTVILPNLKKALWAGALLVFIPSLGEYVIPDLLGGAKNMLYGNLITEQF
LKARDWPFGAALSVLMMLLLLVMALVFRMWEARRARG

Sequences:

>Translated_277_residues
MKLRDLFLLPVGLWFVVFVLLPLIIVAVVSFATRGTYGGVEWSWTLANYVRAFSPTYLGIFAESFKLALITTGVCLVLGV
LVSWAMATASTRLRQLYLMAVVLPFLTNLVIRIYAIRLFVGADGPLQAGLTLLGVPFDSFALTQNKWLVLYGMVTTYLPF
MVLPLYGAFEKFDFSLVEAAQDLGAGIWKILFTVILPNLKKALWAGALLVFIPSLGEYVIPDLLGGAKNMLYGNLITEQF
LKARDWPFGAALSVLMMLLLLVMALVFRMWEARRARG
>Mature_277_residues
MKLRDLFLLPVGLWFVVFVLLPLIIVAVVSFATRGTYGGVEWSWTLANYVRAFSPTYLGIFAESFKLALITTGVCLVLGV
LVSWAMATASTRLRQLYLMAVVLPFLTNLVIRIYAIRLFVGADGPLQAGLTLLGVPFDSFALTQNKWLVLYGMVTTYLPF
MVLPLYGAFEKFDFSLVEAAQDLGAGIWKILFTVILPNLKKALWAGALLVFIPSLGEYVIPDLLGGAKNMLYGNLITEQF
LKARDWPFGAALSVLMMLLLLVMALVFRMWEARRARG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=261, Percent_Identity=41.3793103448276, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1787080, Length=238, Percent_Identity=37.8151260504202, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787713, Length=238, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30790; Mature: 30790

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]