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The map label for this gene is entA [C]

Identifier: 42522872

GI number: 42522872

Start: 1277912

End: 1278649

Strand: Direct

Name: entA [C]

Synonym: Bd1353

Alternate gene names: 42522872

Gene position: 1277912-1278649 (Clockwise)

Preceding gene: 42522870

Following gene: 42522874

Centisome position: 33.78

GC content: 50.95

Gene sequence:

>738_bases
ATGGATTTCAAAGGAAAGAATGTTTTTATCACCGGAGCCAACCGCGGGATCGGTGCCGCTTTAGTAGGGGCCTGCCTCAA
TCGGGGAGTCGCCAAGGTTTACGCTGCCGCCAGAGATAAAAACAAATTACCGAAAAACGATGACCCGCGTGTCGTTCCCG
TTCAACTGGATATCACCAACCGGGAGCAAATAAACGAAGCCGTGTCTGCCGCCGCCGACGTGCAGATCCTGATCAACAAT
GCCGGCACTCTTCATGCCGGAAGTTTTTTGGAAGGGAATAGGGACGGCTTCCTCCGTGACATGCAGGTAAACTATTTCTC
GACGATGGATGTGATGACGGCCTTCGTTCCTGTGCTTAAGCGCAACTGCGACGGTCGTATCGTGAATATCGTGTCGATAG
CCAAGTTCGTGAACTTTCCCTTTATCGCGGGATATTCGGCTTCCAAAGCGGCACTTTATTCGATGACTCAGGCGGCTCGA
ATCGAACTTAGTCCGTACTGTATTGCCGTTCATGCTGTGAATCCGGGTGCCATCGATACGGACATGAATAAAGGTGCCGA
GATGGACATGACCTCTCCGGTCGAGGTTGCAGACAGTATCTTGGCCCACGTCGAAAAAGAGATTTTGGATATTGTTCCGG
ACAAGATAGGTCAGGCGATGTTCAAAGTGTGGCAGGAGTCTCCAGTGGGGTTGGAGAACCTGGCTAAAGATATGTATTTT
AAAAAGCCGGTCCCCTGA

Upstream 100 bases:

>100_bases
ACCGAGCTTTTGGTGTGAACACAACAACGACGATGTCCGTAAATGGCCAGTATTTTAAGTTGAAATATCTTAGCCTGCCT
TGGATCAACCAGGAGGTTTT

Downstream 100 bases:

>100_bases
ACTTCTTATAGGCAGAAAGTCTCTGAACAATCTGCTGACTTGAGGGGTGCTAATAGGTCGACGCAGGATTCAGGGGGATC
GGGGTTCCTATTCACCTTCG

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MDFKGKNVFITGANRGIGAALVGACLNRGVAKVYAAARDKNKLPKNDDPRVVPVQLDITNREQINEAVSAAADVQILINN
AGTLHAGSFLEGNRDGFLRDMQVNYFSTMDVMTAFVPVLKRNCDGRIVNIVSIAKFVNFPFIAGYSASKAALYSMTQAAR
IELSPYCIAVHAVNPGAIDTDMNKGAEMDMTSPVEVADSILAHVEKEILDIVPDKIGQAMFKVWQESPVGLENLAKDMYF
KKPVP

Sequences:

>Translated_245_residues
MDFKGKNVFITGANRGIGAALVGACLNRGVAKVYAAARDKNKLPKNDDPRVVPVQLDITNREQINEAVSAAADVQILINN
AGTLHAGSFLEGNRDGFLRDMQVNYFSTMDVMTAFVPVLKRNCDGRIVNIVSIAKFVNFPFIAGYSASKAALYSMTQAAR
IELSPYCIAVHAVNPGAIDTDMNKGAEMDMTSPVEVADSILAHVEKEILDIVPDKIGQAMFKVWQESPVGLENLAKDMYF
KKPVP
>Mature_245_residues
MDFKGKNVFITGANRGIGAALVGACLNRGVAKVYAAARDKNKLPKNDDPRVVPVQLDITNREQINEAVSAAADVQILINN
AGTLHAGSFLEGNRDGFLRDMQVNYFSTMDVMTAFVPVLKRNCDGRIVNIVSIAKFVNFPFIAGYSASKAALYSMTQAAR
IELSPYCIAVHAVNPGAIDTDMNKGAEMDMTSPVEVADSILAHVEKEILDIVPDKIGQAMFKVWQESPVGLENLAKDMYF
KKPVP

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504503, Length=225, Percent_Identity=29.7777777777778, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI20149619, Length=237, Percent_Identity=31.2236286919831, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI7705925, Length=225, Percent_Identity=28.8888888888889, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI19743808, Length=245, Percent_Identity=26.9387755102041, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4503817, Length=206, Percent_Identity=28.6407766990291, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7706318, Length=189, Percent_Identity=28.042328042328, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI19923817, Length=194, Percent_Identity=27.319587628866, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI109715829, Length=230, Percent_Identity=27.3913043478261, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786812, Length=188, Percent_Identity=28.7234042553192, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787335, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367365, Length=185, Percent_Identity=31.3513513513514, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082160, Length=233, Percent_Identity=27.0386266094421, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788459, Length=192, Percent_Identity=29.6875, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082100, Length=225, Percent_Identity=27.1111111111111, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790717, Length=189, Percent_Identity=26.4550264550265, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17559964, Length=206, Percent_Identity=33.495145631068, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17559962, Length=228, Percent_Identity=30.7017543859649, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557780, Length=197, Percent_Identity=28.4263959390863, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI72001658, Length=208, Percent_Identity=31.25, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71997402, Length=191, Percent_Identity=34.5549738219895, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=202, Percent_Identity=28.7128712871287, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=256, Percent_Identity=29.6875, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24643142, Length=207, Percent_Identity=35.2657004830918, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571526, Length=199, Percent_Identity=32.1608040201005, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357041, Length=205, Percent_Identity=29.7560975609756, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644339, Length=199, Percent_Identity=29.1457286432161, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358495, Length=263, Percent_Identity=29.277566539924, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921754, Length=209, Percent_Identity=29.6650717703349, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007236, Length=234, Percent_Identity=28.6324786324786, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355319, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI23397609, Length=189, Percent_Identity=26.4550264550265, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281364892, Length=234, Percent_Identity=25.2136752136752, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 26609; Mature: 26609

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKGKNVFITGANRGIGAALVGACLNRGVAKVYAAARDKNKLPKNDDPRVVPVQLDITN
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
REQINEAVSAAADVQILINNAGTLHAGSFLEGNRDGFLRDMQVNYFSTMDVMTAFVPVLK
HHHHHHHHHHHCCEEEEEECCCCEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
RNCDGRIVNIVSIAKFVNFPFIAGYSASKAALYSMTQAARIELSPYCIAVHAVNPGAIDT
CCCCCCEEHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHEEEECCEEEEEEECCCCCCCC
DMNKGAEMDMTSPVEVADSILAHVEKEILDIVPDKIGQAMFKVWQESPVGLENLAKDMYF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
KKPVP
CCCCC
>Mature Secondary Structure
MDFKGKNVFITGANRGIGAALVGACLNRGVAKVYAAARDKNKLPKNDDPRVVPVQLDITN
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCC
REQINEAVSAAADVQILINNAGTLHAGSFLEGNRDGFLRDMQVNYFSTMDVMTAFVPVLK
HHHHHHHHHHHCCEEEEEECCCCEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
RNCDGRIVNIVSIAKFVNFPFIAGYSASKAALYSMTQAARIELSPYCIAVHAVNPGAIDT
CCCCCCEEHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHEEEECCEEEEEEECCCCCCCC
DMNKGAEMDMTSPVEVADSILAHVEKEILDIVPDKIGQAMFKVWQESPVGLENLAKDMYF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
KKPVP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA