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The map label for this gene is pcrA [H]

Identifier: 42522842

GI number: 42522842

Start: 1247948

End: 1249966

Strand: Direct

Name: pcrA [H]

Synonym: Bd1321

Alternate gene names: 42522842

Gene position: 1247948-1249966 (Clockwise)

Preceding gene: 42522841

Following gene: 42522843

Centisome position: 32.99

GC content: 51.86

Gene sequence:

>2019_bases
ATGGATTGGTTTAAGGGGCTTAACCCCGAACAACAAAAGGCCGTGAAGCACAACTTCGGGCCTTTGTTGATTTTGGCGGG
TGCCGGTTCAGGCAAAACGACGGTTCTGGTTTCCAGGACAGGACGACTGATTTCAGAGCGTGTGGCACAGGCTCAGGAAA
TCTGCGTGCTTACTTTTACCAACAAAGCCGCGCGCGAGTTGAAACATCGCGTGGGTGCAAAGCTGGGAAGCTCCGGCTCG
GGCATGTGGGCGGGGACCTTCCACTCGTTCGGATTGCAGATTTTGCGCCGATTCCACAAGCATGCGGGGCTTTCCCCGTA
TTTCGGCATTGTTGATCAAAGTGACTGTAATGCCATCGTCAAAGATCTGATCAAAGACATCAAAAACTCGGGCAAAGACA
AGTTCGATGCCGACAAGATCCTGAACATGATCAATGACCGTCGCACGGGCATGGCCCCGCAGACGGAAGCCTTTGACGAA
TATCACGAGATGGTGGAAGTCCTGACTCCGAAATTCACCAAACGCCTGGAGCATCTGGGGGTTGTGGATTTCGAGGGGCT
TTTGATCAAGCCTATTACGTTGTTTAAAGAAAATCCTGAAATCCTGGAAAAAGTGCAGGGCATGTTCACGCAAGTGATGG
TCGATGAGTTCCAGGACACCAATCGTCTGCAGATGGATCTGATCGCGCAGATCGTGAAAAGCCACAACAACATCGCGGTG
GTGGGGGACGACGATCAGTCGATTTACGGCTGGCGTGGGGCGGAAGTGAAGAACATCCTGAACTTCCCCCAGGAATTCAC
ACCGTGTGAAGTGATCAAGCTTGAGCGCAACTATCGTTCTTCCGCTGAAATTCTGGCGGTGGCGAATGCGGCGATTTCCA
AGAATAAAAACCGTCACGGCAAGATCCTGCGTCCGCAGGTTGCGGAAGACACCGGTCAGTTGCCGGAATTGTTTATTTTG
GATCGTGAAGAGGACGAATGTGAATTCGTCGTCACTGAGATTTTGAATTTCCAAAGACAGGGTTATTCCTACAAGGACAT
GGCCGTGCTTTATCGTTCCAATACGCAAGGAGGCTTGATTGAGTCCTCGTTGCGTCGTGCGAACGTGCCCTATCATATCT
CAGGGGGCACTTCGATCTTTGACCGTCGCGAGATCAAGGACCTGATGGCTTATCTGAAGCAGGCTTTGGCTCCGAATGAA
GTGTCCCTTCGCCGTATCATCAATGTGCCTTCGCGCGGGATTGGTGACACTTCCATCGAGAAATTGAGTGAATTTGCACT
GAAAAAACGCATCAACTTCGTCGATGCCTGCCGTTTCTGGCAGGAAGCGGGAGTGCAGGACAAAGCCGGCGCCGCCATTG
ATGATTTGATGATGTTCATTGAAAATCTTCCGAAAAACATCCTCGATTTCCAGGTGGGTTCTTCGCCAGGGGCGAAGATG
GTTCAGATTTTCCAGGACATTGGTTACCGCGAATACGTTTATGGAACGGCCGCGGACCCCACCAGTGCCGAAAAAAAATG
GATGGTCGTCGAGATTCTGGGACGCATCCTGGATTCATTCCTGGGCCGTCGTTCTTATGACGTTGAAAACATCAAATCCT
TCGTGGATTGCATGCTTTTGCGGGATGATCTGTCCGAAGAGGAACTGGAAAACAAAGTTCAGCTGATGACCTTGCACGCC
TCCAAGGGCCTGGAGTTCCCGGTGGTGATTTTGGCGGGCCTTGAAGAAGACCTTCTGCCGCACAAGAATCTGGGATCTGA
CATCGATGAAGAACGTCGTTTGTTTTATGTGGGCGTGACCCGCGCAAAAAAGCGTCTGGTGATGTCGCGCTGTCAGCAGC
GCAAGAAGAACGGCGCGGTTCGTCCGGTGGCGCCTTCACGATTCCTGCTGGAGCTTCCTAAAGAGCTTTACACAGAATTC
CCTCTGGGAGCTCGACCTGTGGTCGGGCAAGAACGCGAGGATTTAGTGGCAAGTTTCCTGTCGAAGCTGGACAGTAAGCT
GGGAACGGGCAAGAAGTAG

Upstream 100 bases:

>100_bases
ATCAAGGACGCTATGACTGATAAAATTGAAGTTGTGTTCGAAGACGAAGTTCGTACTTTGATCCATAACCGTCAGTAATT
TTTTATAGAATCGAAAATTA

Downstream 100 bases:

>100_bases
CGATTTTCTCTCGAGATCCGAATCTATTTTGATCTGAAGCCTGAGCAACCATCACTTTCGTGAATGAGTGCTAAGGCTAT
GTCCGGGAAGTGCCGATCAA

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MDWFKGLNPEQQKAVKHNFGPLLILAGAGSGKTTVLVSRTGRLISERVAQAQEICVLTFTNKAARELKHRVGAKLGSSGS
GMWAGTFHSFGLQILRRFHKHAGLSPYFGIVDQSDCNAIVKDLIKDIKNSGKDKFDADKILNMINDRRTGMAPQTEAFDE
YHEMVEVLTPKFTKRLEHLGVVDFEGLLIKPITLFKENPEILEKVQGMFTQVMVDEFQDTNRLQMDLIAQIVKSHNNIAV
VGDDDQSIYGWRGAEVKNILNFPQEFTPCEVIKLERNYRSSAEILAVANAAISKNKNRHGKILRPQVAEDTGQLPELFIL
DREEDECEFVVTEILNFQRQGYSYKDMAVLYRSNTQGGLIESSLRRANVPYHISGGTSIFDRREIKDLMAYLKQALAPNE
VSLRRIINVPSRGIGDTSIEKLSEFALKKRINFVDACRFWQEAGVQDKAGAAIDDLMMFIENLPKNILDFQVGSSPGAKM
VQIFQDIGYREYVYGTAADPTSAEKKWMVVEILGRILDSFLGRRSYDVENIKSFVDCMLLRDDLSEEELENKVQLMTLHA
SKGLEFPVVILAGLEEDLLPHKNLGSDIDEERRLFYVGVTRAKKRLVMSRCQQRKKNGAVRPVAPSRFLLELPKELYTEF
PLGARPVVGQEREDLVASFLSKLDSKLGTGKK

Sequences:

>Translated_672_residues
MDWFKGLNPEQQKAVKHNFGPLLILAGAGSGKTTVLVSRTGRLISERVAQAQEICVLTFTNKAARELKHRVGAKLGSSGS
GMWAGTFHSFGLQILRRFHKHAGLSPYFGIVDQSDCNAIVKDLIKDIKNSGKDKFDADKILNMINDRRTGMAPQTEAFDE
YHEMVEVLTPKFTKRLEHLGVVDFEGLLIKPITLFKENPEILEKVQGMFTQVMVDEFQDTNRLQMDLIAQIVKSHNNIAV
VGDDDQSIYGWRGAEVKNILNFPQEFTPCEVIKLERNYRSSAEILAVANAAISKNKNRHGKILRPQVAEDTGQLPELFIL
DREEDECEFVVTEILNFQRQGYSYKDMAVLYRSNTQGGLIESSLRRANVPYHISGGTSIFDRREIKDLMAYLKQALAPNE
VSLRRIINVPSRGIGDTSIEKLSEFALKKRINFVDACRFWQEAGVQDKAGAAIDDLMMFIENLPKNILDFQVGSSPGAKM
VQIFQDIGYREYVYGTAADPTSAEKKWMVVEILGRILDSFLGRRSYDVENIKSFVDCMLLRDDLSEEELENKVQLMTLHA
SKGLEFPVVILAGLEEDLLPHKNLGSDIDEERRLFYVGVTRAKKRLVMSRCQQRKKNGAVRPVAPSRFLLELPKELYTEF
PLGARPVVGQEREDLVASFLSKLDSKLGTGKK
>Mature_672_residues
MDWFKGLNPEQQKAVKHNFGPLLILAGAGSGKTTVLVSRTGRLISERVAQAQEICVLTFTNKAARELKHRVGAKLGSSGS
GMWAGTFHSFGLQILRRFHKHAGLSPYFGIVDQSDCNAIVKDLIKDIKNSGKDKFDADKILNMINDRRTGMAPQTEAFDE
YHEMVEVLTPKFTKRLEHLGVVDFEGLLIKPITLFKENPEILEKVQGMFTQVMVDEFQDTNRLQMDLIAQIVKSHNNIAV
VGDDDQSIYGWRGAEVKNILNFPQEFTPCEVIKLERNYRSSAEILAVANAAISKNKNRHGKILRPQVAEDTGQLPELFIL
DREEDECEFVVTEILNFQRQGYSYKDMAVLYRSNTQGGLIESSLRRANVPYHISGGTSIFDRREIKDLMAYLKQALAPNE
VSLRRIINVPSRGIGDTSIEKLSEFALKKRINFVDACRFWQEAGVQDKAGAAIDDLMMFIENLPKNILDFQVGSSPGAKM
VQIFQDIGYREYVYGTAADPTSAEKKWMVVEILGRILDSFLGRRSYDVENIKSFVDCMLLRDDLSEEELENKVQLMTLHA
SKGLEFPVVILAGLEEDLLPHKNLGSDIDEERRLFYVGVTRAKKRLVMSRCQQRKKNGAVRPVAPSRFLLELPKELYTEF
PLGARPVVGQEREDLVASFLSKLDSKLGTGKK

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=667, Percent_Identity=37.0314842578711, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI48994965, Length=652, Percent_Identity=38.1901840490798, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1787196, Length=363, Percent_Identity=26.4462809917355, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=755, Percent_Identity=26.3576158940397, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6324477, Length=669, Percent_Identity=21.6741405082212, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75801; Mature: 75801

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWFKGLNPEQQKAVKHNFGPLLILAGAGSGKTTVLVSRTGRLISERVAQAQEICVLTFT
CCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHEEEEEEC
NKAARELKHRVGAKLGSSGSGMWAGTFHSFGLQILRRFHKHAGLSPYFGIVDQSDCNAIV
CHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
KDLIKDIKNSGKDKFDADKILNMINDRRTGMAPQTEAFDEYHEMVEVLTPKFTKRLEHLG
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCC
VVDFEGLLIKPITLFKENPEILEKVQGMFTQVMVDEFQDTNRLQMDLIAQIVKSHNNIAV
CCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
VGDDDQSIYGWRGAEVKNILNFPQEFTPCEVIKLERNYRSSAEILAVANAAISKNKNRHG
EECCCCCCCCCCCHHHHHHHCCCCCCCCHHEEEEHHCCCCCHHHHHHHHHHHHCCCCCCC
KILRPQVAEDTGQLPELFILDREEDECEFVVTEILNFQRQGYSYKDMAVLYRSNTQGGLI
CCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCHH
ESSLRRANVPYHISGGTSIFDRREIKDLMAYLKQALAPNEVSLRRIINVPSRGIGDTSIE
HHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHH
KLSEFALKKRINFVDACRFWQEAGVQDKAGAAIDDLMMFIENLPKNILDFQVGSSPGAKM
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHEECCCCCCHHH
VQIFQDIGYREYVYGTAADPTSAEKKWMVVEILGRILDSFLGRRSYDVENIKSFVDCMLL
HHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RDDLSEEELENKVQLMTLHASKGLEFPVVILAGLEEDLLPHKNLGSDIDEERRLFYVGVT
HHCCCHHHHHCCEEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCEEEEEEHH
RAKKRLVMSRCQQRKKNGAVRPVAPSRFLLELPKELYTEFPLGARPVVGQEREDLVASFL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHH
SKLDSKLGTGKK
HHHHHHCCCCCC
>Mature Secondary Structure
MDWFKGLNPEQQKAVKHNFGPLLILAGAGSGKTTVLVSRTGRLISERVAQAQEICVLTFT
CCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHEEEEEEC
NKAARELKHRVGAKLGSSGSGMWAGTFHSFGLQILRRFHKHAGLSPYFGIVDQSDCNAIV
CHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
KDLIKDIKNSGKDKFDADKILNMINDRRTGMAPQTEAFDEYHEMVEVLTPKFTKRLEHLG
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCC
VVDFEGLLIKPITLFKENPEILEKVQGMFTQVMVDEFQDTNRLQMDLIAQIVKSHNNIAV
CCCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
VGDDDQSIYGWRGAEVKNILNFPQEFTPCEVIKLERNYRSSAEILAVANAAISKNKNRHG
EECCCCCCCCCCCHHHHHHHCCCCCCCCHHEEEEHHCCCCCHHHHHHHHHHHHCCCCCCC
KILRPQVAEDTGQLPELFILDREEDECEFVVTEILNFQRQGYSYKDMAVLYRSNTQGGLI
CCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCHH
ESSLRRANVPYHISGGTSIFDRREIKDLMAYLKQALAPNEVSLRRIINVPSRGIGDTSIE
HHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHH
KLSEFALKKRINFVDACRFWQEAGVQDKAGAAIDDLMMFIENLPKNILDFQVGSSPGAKM
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHEECCCCCCHHH
VQIFQDIGYREYVYGTAADPTSAEKKWMVVEILGRILDSFLGRRSYDVENIKSFVDCMLL
HHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
RDDLSEEELENKVQLMTLHASKGLEFPVVILAGLEEDLLPHKNLGSDIDEERRLFYVGVT
HHCCCHHHHHCCEEEEEEECCCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCEEEEEEHH
RAKKRLVMSRCQQRKKNGAVRPVAPSRFLLELPKELYTEFPLGARPVVGQEREDLVASFL
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHH
SKLDSKLGTGKK
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]