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The map label for this gene is nifR [H]

Identifier: 42522830

GI number: 42522830

Start: 1236884

End: 1237942

Strand: Direct

Name: nifR [H]

Synonym: Bd1309

Alternate gene names: 42522830

Gene position: 1236884-1237942 (Clockwise)

Preceding gene: 42522829

Following gene: 42522832

Centisome position: 32.7

GC content: 55.71

Gene sequence:

>1059_bases
GTGAAACTGGGGCTGCATCGTCCGGTTCTGGACGGTAAAGTGAACTTCCCGTTGTGTCTGGCCCCGATGGTGGGCCTGAC
TCACGTGGCTTTGCGCGAAGTCATGCGCGATTATCTTCCGGCCGAGGCTTACACCATCTGGCCGACCGAGATGTTGAATT
CCCGCCGTATTCCTGGCGAAAATCTTGAAAAAACCCCTGAAACCATGCGCGCCGCTTATGAGCCGGGCTTGGTGCCACAG
ATCCTTGGTAACGAAGAAGATGCCATTGCTGAAAGCGTGAAACGCCTGGTGGAGTGGGGCGCTGAAGCCATCGACATCAA
CATGGGCTGCCCGGTGCAAAAAGCGCTGAAGCACAATTATGGTGTGGCGTTGATGGGGGACCCGGCCTATGCCGCCGAAG
TGGTGCGCATGACCGTCAAAAATTCCACCGTGCCTGTCAGTGTGAAGTTGCGCGCTGTCGGCAGCACGAAAGAGTTCGAC
GAACTTTTGACGTTCGTGTCGGGTCTTCGTCAGTCGGGCGCCGCCTGGGTGTGTCTGCATCCGCGAACAGCGGCGCAAAA
ACGCCGTGGTAATGCTGATTGGGAGCAGATCAAACAACTTCATAAAGCGGTCGATTTCCCGGTCATCGGCAATGGCGACG
TACAAACCTATGACGACGCCATCAACATGCTGAAAGAAACCGGTTGTGACATGGCGATGGCGGGTCGTGGTCTTGCCGCG
CGCCCGTGGATGATGTGGCAACTGGGTGAAGAGCTGGGTTTTGCGCCCCCCGCAGGTAAAGAAGGTCAGAAAGCCCCTCG
CACTTCGGAAGAAGAGGGCGCCGAGTACGGCAAATGTTTGTTGCGATTGATTGATCGCTGTCGTTTTTATTTCGGGGAAG
ATCTGGCTATGCGCAAAGTGCGCTTCTATGTTCGCACCACAAGTGTGTGGCTGCCGTTTGGAAACACTCTGGTGGGTGTT
TGTGCGAAGGCTCGCACCATTGATGAAATGATCGAGGGTGTGACGAAATTCTTTGAAGGCCCCGTGGAGATGAGTCTGCG
CACGGAGCTTCGACAATAA

Upstream 100 bases:

>100_bases
ATCTACGGAGACTTGTTCTTCTCTGGTTTGTTGTTCGGTGGCTATGAAGCCATCAAAAAATACGCGCCAGAATTCGTCGG
CGCTCCTGTAAAATAAGACT

Downstream 100 bases:

>100_bases
TCCTACTCTTGCAGCAGGGGCATCAGTTTGCCTTCTTCGTCCAAGGCTTTCAAATCCGTATAGCCGCCGATCAATTTGCC
GTTGATCATGATGATCGGCA

Product: nitrogen regulation protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKLGLHRPVLDGKVNFPLCLAPMVGLTHVALREVMRDYLPAEAYTIWPTEMLNSRRIPGENLEKTPETMRAAYEPGLVPQ
ILGNEEDAIAESVKRLVEWGAEAIDINMGCPVQKALKHNYGVALMGDPAYAAEVVRMTVKNSTVPVSVKLRAVGSTKEFD
ELLTFVSGLRQSGAAWVCLHPRTAAQKRRGNADWEQIKQLHKAVDFPVIGNGDVQTYDDAINMLKETGCDMAMAGRGLAA
RPWMMWQLGEELGFAPPAGKEGQKAPRTSEEEGAEYGKCLLRLIDRCRFYFGEDLAMRKVRFYVRTTSVWLPFGNTLVGV
CAKARTIDEMIEGVTKFFEGPVEMSLRTELRQ

Sequences:

>Translated_352_residues
MKLGLHRPVLDGKVNFPLCLAPMVGLTHVALREVMRDYLPAEAYTIWPTEMLNSRRIPGENLEKTPETMRAAYEPGLVPQ
ILGNEEDAIAESVKRLVEWGAEAIDINMGCPVQKALKHNYGVALMGDPAYAAEVVRMTVKNSTVPVSVKLRAVGSTKEFD
ELLTFVSGLRQSGAAWVCLHPRTAAQKRRGNADWEQIKQLHKAVDFPVIGNGDVQTYDDAINMLKETGCDMAMAGRGLAA
RPWMMWQLGEELGFAPPAGKEGQKAPRTSEEEGAEYGKCLLRLIDRCRFYFGEDLAMRKVRFYVRTTSVWLPFGNTLVGV
CAKARTIDEMIEGVTKFFEGPVEMSLRTELRQ
>Mature_352_residues
MKLGLHRPVLDGKVNFPLCLAPMVGLTHVALREVMRDYLPAEAYTIWPTEMLNSRRIPGENLEKTPETMRAAYEPGLVPQ
ILGNEEDAIAESVKRLVEWGAEAIDINMGCPVQKALKHNYGVALMGDPAYAAEVVRMTVKNSTVPVSVKLRAVGSTKEFD
ELLTFVSGLRQSGAAWVCLHPRTAAQKRRGNADWEQIKQLHKAVDFPVIGNGDVQTYDDAINMLKETGCDMAMAGRGLAA
RPWMMWQLGEELGFAPPAGKEGQKAPRTSEEEGAEYGKCLLRLIDRCRFYFGEDLAMRKVRFYVRTTSVWLPFGNTLVGV
CAKARTIDEMIEGVTKFFEGPVEMSLRTELRQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=28.9473684210526, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI40807366, Length=245, Percent_Identity=28.1632653061224, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI239788483, Length=163, Percent_Identity=33.1288343558282, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI239788462, Length=163, Percent_Identity=33.1288343558282, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI8923374, Length=247, Percent_Identity=27.5303643724696, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789660, Length=236, Percent_Identity=31.3559322033898, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=29.1304347826087, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=230, Percent_Identity=26.5217391304348, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=228, Percent_Identity=23.2456140350877, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=250, Percent_Identity=25.6, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=229, Percent_Identity=27.0742358078603, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19921524, Length=257, Percent_Identity=27.2373540856031, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45549423, Length=159, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24580595, Length=243, Percent_Identity=26.3374485596708, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19920448, Length=243, Percent_Identity=26.3374485596708, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24585320, Length=266, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39222; Mature: 39222

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLGLHRPVLDGKVNFPLCLAPMVGLTHVALREVMRDYLPAEAYTIWPTEMLNSRRIPGE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHCCCCCCCC
NLEKTPETMRAAYEPGLVPQILGNEEDAIAESVKRLVEWGAEAIDINMGCPVQKALKHNY
CCCCCHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCC
GVALMGDPAYAAEVVRMTVKNSTVPVSVKLRAVGSTKEFDELLTFVSGLRQSGAAWVCLH
CEEEECCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEC
PRTAAQKRRGNADWEQIKQLHKAVDFPVIGNGDVQTYDDAINMLKETGCDMAMAGRGLAA
CCHHHHHHCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCC
RPWMMWQLGEELGFAPPAGKEGQKAPRTSEEEGAEYGKCLLRLIDRCRFYFGEDLAMRKV
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RFYVRTTSVWLPFGNTLVGVCAKARTIDEMIEGVTKFFEGPVEMSLRTELRQ
HHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLGLHRPVLDGKVNFPLCLAPMVGLTHVALREVMRDYLPAEAYTIWPTEMLNSRRIPGE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHCCCCCCCC
NLEKTPETMRAAYEPGLVPQILGNEEDAIAESVKRLVEWGAEAIDINMGCPVQKALKHNY
CCCCCHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCC
GVALMGDPAYAAEVVRMTVKNSTVPVSVKLRAVGSTKEFDELLTFVSGLRQSGAAWVCLH
CEEEECCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEC
PRTAAQKRRGNADWEQIKQLHKAVDFPVIGNGDVQTYDDAINMLKETGCDMAMAGRGLAA
CCHHHHHHCCCCCHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCCCHHHHCCCCCC
RPWMMWQLGEELGFAPPAGKEGQKAPRTSEEEGAEYGKCLLRLIDRCRFYFGEDLAMRKV
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RFYVRTTSVWLPFGNTLVGVCAKARTIDEMIEGVTKFFEGPVEMSLRTELRQ
HHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]