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The map label for this gene is mltD [H]

Identifier: 42522673

GI number: 42522673

Start: 1061477

End: 1063057

Strand: Direct

Name: mltD [H]

Synonym: Bd1125

Alternate gene names: 42522673

Gene position: 1061477-1063057 (Clockwise)

Preceding gene: 42522672

Following gene: 42522674

Centisome position: 28.06

GC content: 51.04

Gene sequence:

>1581_bases
ATGAGGAGACTTTTAACATTGAGTTTGGTCCTGGCCTTTGTGTCAGGGTGTGCTCATAAGGATTTGGGCACAAAAGCTGG
CAGACCAGCTACCAACAGTGAAGGGGATTTGAAGGACATCGGTTCGTTCCGTTTGTCTGACCCCGAAGGCCCCAAGGTGG
TGGATCAGGAATTGGAATCCATCCCTACTGAAGTTAACCCCCTAGTTGAAAAATGGATTGCCTACTTCCAGGGTCGCGGT
CGCCCGCACATGGAACGTTACCTGGCCCGTTCCACTCGTTACGAAAAACTGATGAAGAAGGTTTTGCGTGACAATGGACT
TCCTGAAGATCTGTTTTACATCGCTTTGATCGAATCCGGATTCAGCTCGCGCGCGACCTCTCACGCTTCAGCGGTGGGGT
ACTGGCAGTTCATCCGTGGTACGGGCAAACGTTATGGTCTGGACATCAATGCCTTCGTGGATGAGCGTCGTGACCCGGTC
TTTGCAACTCAGGCTGCGGCCGAGTACTTCAAAGGTCTTTATAGCGTGTTTGGTTCCTGGTATCTGGCGATGGCTTCCTA
CAACGTGGGTGAAAATCGCGTGAAGCGTGAAGTGATGAATCACTACACCCGTGACTTCTGGGAGCTGGCTCGTAAAAAAC
GTCTGCCAGCTGAAACCATCAACTACGTGCCAAAATTCATCGCGGCTAAAATGATCGGTAAGGATCCGGCGAAGTACGGG
TTTGACGACATCGATTATCTTCCGCCGATTGAGTTCGATCACATCACCGTTTCCCAGCCAGTGAACTTGCGTCAGATGGC
CGAGAAAATGAACCTGAACTATGAGGACTTCAAAGCTTTGAATCCCAAGTTCAAGGGTGAAGTGGCGATCCTGAAGGGCA
GTGACCTGATTCTGCGTATTCCTCCGGGCACGACAGAGCAATCCAAAGTGGCTGCGGCAGAAAGTTTTGTTTCCCGTGTT
CAGTTTGTGGCTGACACTGGTGACACGCAGACCTACCGCATACGTCATGGTGACACTTTGAGCACGGTGGCTCGTCGTTA
TCGCACGACGGTGGCATTCCTGCGTGACCTGAATGATCTGCCTCGTCGCAAGCCCTTGAAAGTGGGCATGCGCATCCAGG
TTCCGGATCGTACGCCGCTGAAGGACCGTTCACAAACTCGTTCTATGGTGGCAAAGAAATCCGATGCTCCTAAGAACACA
GTGACAATCAGCAGTGATGGTCGTTACTACATCGTTCAGTCCGGGGACTCTTTGTTCACGATTGCGCGCAAATATGCCAC
CAGCGTTTCTGAGCTTCAGCGTATGAACAACATCAAACGTGGTCGCACATTGAAAGTGGGCATGAAACTGAAAGTCCCAA
CTCCAGGCAGCTCGTCTGCACGTGAAGTGGCTACTGAAGTTGCTCAAAGCAAAAAAGCGAAATCCAAAGTTCACGTGGTT
CGCAGAGGTGAAAACCTTTCTGTGATCGCGGCGAAATACAATGTGGCAGTTTCTGATATCAAAGAAAGAAACAAGATCCG
CAACCCGGCTTCTCTGGTTGTGGGGGCACGCATCGTGATCCCATCGGAAGTGAAGCAATAA

Upstream 100 bases:

>100_bases
CTTATGGCAGGATTTGCTTAACAAAAAGGCAAAATAAGGGCGATGTTTTATTGAAAAATCCCTCTGAGTTTTGTAGCCTG
AAAACTCAGTCGGAGGACTC

Downstream 100 bases:

>100_bases
GACTTTTCTCGCAATTGTCTTAACTCGACCCCAAAATCACGACGGGAAGTAAAGCCGGTCTCAGATTTTGGGGTCTTTTT
TTATCTTCTCTCAGGTTCTA

Product: membrane-bound lytic murein transglycosylase D precursor

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MRRLLTLSLVLAFVSGCAHKDLGTKAGRPATNSEGDLKDIGSFRLSDPEGPKVVDQELESIPTEVNPLVEKWIAYFQGRG
RPHMERYLARSTRYEKLMKKVLRDNGLPEDLFYIALIESGFSSRATSHASAVGYWQFIRGTGKRYGLDINAFVDERRDPV
FATQAAAEYFKGLYSVFGSWYLAMASYNVGENRVKREVMNHYTRDFWELARKKRLPAETINYVPKFIAAKMIGKDPAKYG
FDDIDYLPPIEFDHITVSQPVNLRQMAEKMNLNYEDFKALNPKFKGEVAILKGSDLILRIPPGTTEQSKVAAAESFVSRV
QFVADTGDTQTYRIRHGDTLSTVARRYRTTVAFLRDLNDLPRRKPLKVGMRIQVPDRTPLKDRSQTRSMVAKKSDAPKNT
VTISSDGRYYIVQSGDSLFTIARKYATSVSELQRMNNIKRGRTLKVGMKLKVPTPGSSSAREVATEVAQSKKAKSKVHVV
RRGENLSVIAAKYNVAVSDIKERNKIRNPASLVVGARIVIPSEVKQ

Sequences:

>Translated_526_residues
MRRLLTLSLVLAFVSGCAHKDLGTKAGRPATNSEGDLKDIGSFRLSDPEGPKVVDQELESIPTEVNPLVEKWIAYFQGRG
RPHMERYLARSTRYEKLMKKVLRDNGLPEDLFYIALIESGFSSRATSHASAVGYWQFIRGTGKRYGLDINAFVDERRDPV
FATQAAAEYFKGLYSVFGSWYLAMASYNVGENRVKREVMNHYTRDFWELARKKRLPAETINYVPKFIAAKMIGKDPAKYG
FDDIDYLPPIEFDHITVSQPVNLRQMAEKMNLNYEDFKALNPKFKGEVAILKGSDLILRIPPGTTEQSKVAAAESFVSRV
QFVADTGDTQTYRIRHGDTLSTVARRYRTTVAFLRDLNDLPRRKPLKVGMRIQVPDRTPLKDRSQTRSMVAKKSDAPKNT
VTISSDGRYYIVQSGDSLFTIARKYATSVSELQRMNNIKRGRTLKVGMKLKVPTPGSSSAREVATEVAQSKKAKSKVHVV
RRGENLSVIAAKYNVAVSDIKERNKIRNPASLVVGARIVIPSEVKQ
>Mature_526_residues
MRRLLTLSLVLAFVSGCAHKDLGTKAGRPATNSEGDLKDIGSFRLSDPEGPKVVDQELESIPTEVNPLVEKWIAYFQGRG
RPHMERYLARSTRYEKLMKKVLRDNGLPEDLFYIALIESGFSSRATSHASAVGYWQFIRGTGKRYGLDINAFVDERRDPV
FATQAAAEYFKGLYSVFGSWYLAMASYNVGENRVKREVMNHYTRDFWELARKKRLPAETINYVPKFIAAKMIGKDPAKYG
FDDIDYLPPIEFDHITVSQPVNLRQMAEKMNLNYEDFKALNPKFKGEVAILKGSDLILRIPPGTTEQSKVAAAESFVSRV
QFVADTGDTQTYRIRHGDTLSTVARRYRTTVAFLRDLNDLPRRKPLKVGMRIQVPDRTPLKDRSQTRSMVAKKSDAPKNT
VTISSDGRYYIVQSGDSLFTIARKYATSVSELQRMNNIKRGRTLKVGMKLKVPTPGSSSAREVATEVAQSKKAKSKVHVV
RRGENLSVIAAKYNVAVSDIKERNKIRNPASLVVGARIVIPSEVKQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=388, Percent_Identity=28.6082474226804, Blast_Score=146, Evalue=4e-36,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 59246; Mature: 59246

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLLTLSLVLAFVSGCAHKDLGTKAGRPATNSEGDLKDIGSFRLSDPEGPKVVDQELES
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCCHHHHHHHHH
IPTEVNPLVEKWIAYFQGRGRPHMERYLARSTRYEKLMKKVLRDNGLPEDLFYIALIESG
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
FSSRATSHASAVGYWQFIRGTGKRYGLDINAFVDERRDPVFATQAAAEYFKGLYSVFGSW
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YLAMASYNVGENRVKREVMNHYTRDFWELARKKRLPAETINYVPKFIAAKMIGKDPAKYG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCC
FDDIDYLPPIEFDHITVSQPVNLRQMAEKMNLNYEDFKALNPKFKGEVAILKGSDLILRI
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCEEEEC
PPGTTEQSKVAAAESFVSRVQFVADTGDTQTYRIRHGDTLSTVARRYRTTVAFLRDLNDL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
PRRKPLKVGMRIQVPDRTPLKDRSQTRSMVAKKSDAPKNTVTISSDGRYYIVQSGDSLFT
CCCCCCEECCEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCHHHH
IARKYATSVSELQRMNNIKRGRTLKVGMKLKVPTPGSSSAREVATEVAQSKKAKSKVHVV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHE
RRGENLSVIAAKYNVAVSDIKERNKIRNPASLVVGARIVIPSEVKQ
ECCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHEEEEEECCHHCCC
>Mature Secondary Structure
MRRLLTLSLVLAFVSGCAHKDLGTKAGRPATNSEGDLKDIGSFRLSDPEGPKVVDQELES
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCCHHHHHHHHH
IPTEVNPLVEKWIAYFQGRGRPHMERYLARSTRYEKLMKKVLRDNGLPEDLFYIALIESG
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
FSSRATSHASAVGYWQFIRGTGKRYGLDINAFVDERRDPVFATQAAAEYFKGLYSVFGSW
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
YLAMASYNVGENRVKREVMNHYTRDFWELARKKRLPAETINYVPKFIAAKMIGKDPAKYG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCC
FDDIDYLPPIEFDHITVSQPVNLRQMAEKMNLNYEDFKALNPKFKGEVAILKGSDLILRI
CCCCCCCCCCCCCCEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCEEEEC
PPGTTEQSKVAAAESFVSRVQFVADTGDTQTYRIRHGDTLSTVARRYRTTVAFLRDLNDL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
PRRKPLKVGMRIQVPDRTPLKDRSQTRSMVAKKSDAPKNTVTISSDGRYYIVQSGDSLFT
CCCCCCEECCEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCHHHH
IARKYATSVSELQRMNNIKRGRTLKVGMKLKVPTPGSSSAREVATEVAQSKKAKSKVHVV
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHE
RRGENLSVIAAKYNVAVSDIKERNKIRNPASLVVGARIVIPSEVKQ
ECCCCCEEEEEEECHHHHHHHHHHHCCCCHHHHEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]