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The map label for this gene is tpiA

Identifier: 42522607

GI number: 42522607

Start: 996729

End: 997454

Strand: Direct

Name: tpiA

Synonym: Bd1051

Alternate gene names: 42522607

Gene position: 996729-997454 (Clockwise)

Preceding gene: 42522606

Following gene: 42522611

Centisome position: 26.35

GC content: 49.86

Gene sequence:

>726_bases
ATGAAAAAGATTTTTGCTGCTAACTGGAAGCTTTTTAAATCTCCAAAAGAAACGCGTGAGTTTTTTGGTCAGTTCAAAGA
ACTGGCGGGGAAAGCAACGGGGGAAGTGGTCTTTTTCCCTTCAGCGATTTCTTTGGAAGCAGCCAGTGAATCTTTGAAAG
GTACAAGCATCAAGTTTGGCGCTCAGAACTGTTACTTCCAGGCACAAGGGGCTTTCACGGGTGAAAACTCGGCTCAGGTG
GTTAAAGATCTGGGGGGCAGCTATGTATTGATCGGTCACAGCGAACGCCGTGCGATCTTTGGTGAAGGTGATGCGCTTGT
GGCAGACAAAGTGGCTTTCGTTCAAGGTCTGGGCCTGACGCCGATGTTGTGCATCGGTGAAACCCTGCAAGAACGCGAAT
CCGCGAAGACCTTCCGTGTTCTGGAAACTCAGCTAAATCTGGGTTTGGCGAAGGCCGACAAAACCAAGCCAGTGGTTGTT
GCCTATGAGCCGGTTTGGGCGATCGGGACGGGGAAAGTGGCGACACCAGAGCAGGTGGCAGAAACTCACACAGACGTTTT
CAATATTCTAAAAGCACTGGGTTTTGAAACGGCACCGATTCTTTATGGTGGCAGCGTAAAACCGGACAATGCCGCTGGTT
TGATCAAACAACCGCATGTGAATGGTTTCCTGGTTGGGGGAGCTTCTTTGGAAGCAAAGTCCTTCAGCGAGATTGCTTCC
GTTTAG

Upstream 100 bases:

>100_bases
GGCCTTCTCGCTCCGACGTGCTTGGAGCACGCCTGCGCTGCGAGGGCCCACCTCCGCCTTGCATCTGAACCCTTTTGACC
AGCCTTAAAGGATAGACGTT

Downstream 100 bases:

>100_bases
GATTTAACCGTTGCCGTAGCGCTTTTGGCGCGCGGCGGATTTAACAGCGTCTTCGACCAGTTTGGCGTCAGACGGGGAAA
GCTTCTTCAGTGATTCGAGG

Product: hypothetical protein

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MKKIFAANWKLFKSPKETREFFGQFKELAGKATGEVVFFPSAISLEAASESLKGTSIKFGAQNCYFQAQGAFTGENSAQV
VKDLGGSYVLIGHSERRAIFGEGDALVADKVAFVQGLGLTPMLCIGETLQERESAKTFRVLETQLNLGLAKADKTKPVVV
AYEPVWAIGTGKVATPEQVAETHTDVFNILKALGFETAPILYGGSVKPDNAAGLIKQPHVNGFLVGGASLEAKSFSEIAS
V

Sequences:

>Translated_241_residues
MKKIFAANWKLFKSPKETREFFGQFKELAGKATGEVVFFPSAISLEAASESLKGTSIKFGAQNCYFQAQGAFTGENSAQV
VKDLGGSYVLIGHSERRAIFGEGDALVADKVAFVQGLGLTPMLCIGETLQERESAKTFRVLETQLNLGLAKADKTKPVVV
AYEPVWAIGTGKVATPEQVAETHTDVFNILKALGFETAPILYGGSVKPDNAAGLIKQPHVNGFLVGGASLEAKSFSEIAS
V
>Mature_241_residues
MKKIFAANWKLFKSPKETREFFGQFKELAGKATGEVVFFPSAISLEAASESLKGTSIKFGAQNCYFQAQGAFTGENSAQV
VKDLGGSYVLIGHSERRAIFGEGDALVADKVAFVQGLGLTPMLCIGETLQERESAKTFRVLETQLNLGLAKADKTKPVVV
AYEPVWAIGTGKVATPEQVAETHTDVFNILKALGFETAPILYGGSVKPDNAAGLIKQPHVNGFLVGGASLEAKSFSEIAS
V

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=228, Percent_Identity=42.5438596491228, Blast_Score=166, Evalue=1e-41,
Organism=Homo sapiens, GI226529917, Length=235, Percent_Identity=42.1276595744681, Blast_Score=166, Evalue=2e-41,
Organism=Escherichia coli, GI1790353, Length=237, Percent_Identity=45.5696202531646, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17536593, Length=229, Percent_Identity=43.6681222707424, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6320255, Length=228, Percent_Identity=44.7368421052632, Blast_Score=183, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28572008, Length=229, Percent_Identity=45.8515283842795, Blast_Score=173, Evalue=8e-44,
Organism=Drosophila melanogaster, GI28572006, Length=229, Percent_Identity=45.8515283842795, Blast_Score=173, Evalue=8e-44,
Organism=Drosophila melanogaster, GI28572004, Length=229, Percent_Identity=45.8515283842795, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_BDEBA (Q6MP18)

Other databases:

- EMBL:   BX842648
- RefSeq:   NP_967987.1
- HSSP:   P36204
- ProteinModelPortal:   Q6MP18
- SMR:   Q6MP18
- GeneID:   2734419
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1051
- NMPDR:   fig|264462.1.peg.957
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q6MP18
- BioCyc:   BBAC264462:BD1051-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25681; Mature: 25681

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 93-93 ACT_SITE 163-163 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIFAANWKLFKSPKETREFFGQFKELAGKATGEVVFFPSAISLEAASESLKGTSIKFG
CCCEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEHHHHHHCCCCCEEEEC
AQNCYFQAQGAFTGENSAQVVKDLGGSYVLIGHSERRAIFGEGDALVADKVAFVQGLGLT
CCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCEEECCCCEEHHHHHHHHHCCCCC
PMLCIGETLQERESAKTFRVLETQLNLGLAKADKTKPVVVAYEPVWAIGTGKVATPEQVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECCEEEECCCCCCCHHHHH
ETHTDVFNILKALGFETAPILYGGSVKPDNAAGLIKQPHVNGFLVGGASLEAKSFSEIAS
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHC
V
C
>Mature Secondary Structure
MKKIFAANWKLFKSPKETREFFGQFKELAGKATGEVVFFPSAISLEAASESLKGTSIKFG
CCCEEECCCCEECCCHHHHHHHHHHHHHHCCCCCCEEEECCCEEHHHHHHCCCCCEEEEC
AQNCYFQAQGAFTGENSAQVVKDLGGSYVLIGHSERRAIFGEGDALVADKVAFVQGLGLT
CCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCEEECCCCEEHHHHHHHHHCCCCC
PMLCIGETLQERESAKTFRVLETQLNLGLAKADKTKPVVVAYEPVWAIGTGKVATPEQVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEECCEEEECCCCCCCHHHHH
ETHTDVFNILKALGFETAPILYGGSVKPDNAAGLIKQPHVNGFLVGGASLEAKSFSEIAS
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA