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The map label for this gene is pgk

Identifier: 42522606

GI number: 42522606

Start: 995381

End: 996586

Strand: Direct

Name: pgk

Synonym: Bd1050

Alternate gene names: 42522606

Gene position: 995381-996586 (Clockwise)

Preceding gene: 42522605

Following gene: 42522607

Centisome position: 26.31

GC content: 50.33

Gene sequence:

>1206_bases
ATGGCTAACGGTCTTAAAGGCATCAAGACAGTTCGTGATTTCGAGTTGGCGGGAAAAGTTGTTTTCCTGCGCTTGGATTT
GAACGTTCCAATGGAGAACGGCAAGATCACGGATGAAAATCGCATCACGGCATCTTTGCCGACAATCAAGTATTGCATGG
AGCAGGGAGCGAAGCTGGTAATGGCTTCTCACCTGGGCCGTCCAAAAACCAAAGACGACACAGAGTTTTCTTTGGAGCCA
GTGGCAAAACGCCTGCAGGACCTTTTGAATGCCGAAGTGATTCTGGTGGAAGAACCGGACTCTGACGCTCCAAAACACCT
GCTTCCATCTTTGAAGCCTCATCAGCTGATCCTTCTTGAAAACGTGCGTTTTGAAGAAGGGGAGACGAAGGACTCTGTTG
AGTTCGCGCAAAAGATCGCCAACTACAGCGACATTTACATCAATGACGCTTTCGGCGCTTCTCACCGCGCTCACGCGACC
ATTCACGCTTTGCCTTCAGTGATGAAGGACAAAGGTATCGGCTTCCTGATTGAAAAGGAAATCACGATGCTGGATTCCCT
TCTGCAAAACCCAAAACGCCCGTACATCGCGGTGATGGGTGGCGCGAAGGTTTCTGACAAGATCGCGGTTATTGAACGCC
TTATGGACGTTGTTGACGGTTTCATCGTGGGTGGCGCGATGGCGTACACTTTCCTGAAAGCTCAAGGCCTGCCAGTTGGT
AAGTCTTTGGTTGAAAATGACAAATTGAAATACGCCAAAGAAATGATCGAGCGTATCGAAGCCCGCAACAAAACCATTTT
GTTGCCGGTGGATCACGTGGCGACCAAAGGCATCACAGACACAGCGCACGCGCATGTGACCAATGATGTGGCGATTGCGG
AAGACGAATTGGGTGTGGACATCGGTCCTAAATCCATCAAGAACTTCTCTGCGGCTTTGCGTGAAGCAGGCACTATTTTC
TGGAACGGTCCTATGGGCATCTTTGAAAATCCGGCTTTTGCAAAAGGCACCTTCGGCGTGGCTCAGGCCATTGCTGACAG
CGAAGCGATCAAAATCGTGGGTGGTGGTGATTCTGCAGCGGCAGCGGAAGCTTCCGGCTTTGCAGGTAAAATGACCCACA
TCTCCACTGGTGGTGGCGCATCCTTGGAATACTTGCAGGGCGATAAATTGCCAGGACTTGAAATTTTAAGAACTCGCATC
CGCTAA

Upstream 100 bases:

>100_bases
TCGCATGGTGAAAGTTCTTTCCTGGTACGACAATGAAACTGGCTTCTCCAACCGTATGGTGGACGTGGCCCTGCACATGC
AGAAGAAAGGTCTTTAATTC

Downstream 100 bases:

>100_bases
GGACGGCCGGTGAAAAGGGCCCATCTGACTGCGTTGTCAGCGGGCCTTCTCGCTCCGACGTGCTTGGAGCACGCCTGCGC
TGCGAGGGCCCACCTCCGCC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MANGLKGIKTVRDFELAGKVVFLRLDLNVPMENGKITDENRITASLPTIKYCMEQGAKLVMASHLGRPKTKDDTEFSLEP
VAKRLQDLLNAEVILVEEPDSDAPKHLLPSLKPHQLILLENVRFEEGETKDSVEFAQKIANYSDIYINDAFGASHRAHAT
IHALPSVMKDKGIGFLIEKEITMLDSLLQNPKRPYIAVMGGAKVSDKIAVIERLMDVVDGFIVGGAMAYTFLKAQGLPVG
KSLVENDKLKYAKEMIERIEARNKTILLPVDHVATKGITDTAHAHVTNDVAIAEDELGVDIGPKSIKNFSAALREAGTIF
WNGPMGIFENPAFAKGTFGVAQAIADSEAIKIVGGGDSAAAAEASGFAGKMTHISTGGGASLEYLQGDKLPGLEILRTRI
R

Sequences:

>Translated_401_residues
MANGLKGIKTVRDFELAGKVVFLRLDLNVPMENGKITDENRITASLPTIKYCMEQGAKLVMASHLGRPKTKDDTEFSLEP
VAKRLQDLLNAEVILVEEPDSDAPKHLLPSLKPHQLILLENVRFEEGETKDSVEFAQKIANYSDIYINDAFGASHRAHAT
IHALPSVMKDKGIGFLIEKEITMLDSLLQNPKRPYIAVMGGAKVSDKIAVIERLMDVVDGFIVGGAMAYTFLKAQGLPVG
KSLVENDKLKYAKEMIERIEARNKTILLPVDHVATKGITDTAHAHVTNDVAIAEDELGVDIGPKSIKNFSAALREAGTIF
WNGPMGIFENPAFAKGTFGVAQAIADSEAIKIVGGGDSAAAAEASGFAGKMTHISTGGGASLEYLQGDKLPGLEILRTRI
R
>Mature_400_residues
ANGLKGIKTVRDFELAGKVVFLRLDLNVPMENGKITDENRITASLPTIKYCMEQGAKLVMASHLGRPKTKDDTEFSLEPV
AKRLQDLLNAEVILVEEPDSDAPKHLLPSLKPHQLILLENVRFEEGETKDSVEFAQKIANYSDIYINDAFGASHRAHATI
HALPSVMKDKGIGFLIEKEITMLDSLLQNPKRPYIAVMGGAKVSDKIAVIERLMDVVDGFIVGGAMAYTFLKAQGLPVGK
SLVENDKLKYAKEMIERIEARNKTILLPVDHVATKGITDTAHAHVTNDVAIAEDELGVDIGPKSIKNFSAALREAGTIFW
NGPMGIFENPAFAKGTFGVAQAIADSEAIKIVGGGDSAAAAEASGFAGKMTHISTGGGASLEYLQGDKLPGLEILRTRIR

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=409, Percent_Identity=37.8973105134474, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI31543397, Length=409, Percent_Identity=38.6308068459658, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1789294, Length=390, Percent_Identity=42.8205128205128, Blast_Score=295, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=40.5405405405405, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6319857, Length=410, Percent_Identity=39.5121951219512, Blast_Score=293, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24581203, Length=409, Percent_Identity=39.8533007334963, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI17136394, Length=410, Percent_Identity=37.3170731707317, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_BDEBA (P62410)

Other databases:

- EMBL:   BX842648
- RefSeq:   NP_967986.1
- ProteinModelPortal:   P62410
- SMR:   P62410
- GeneID:   2734418
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1050
- NMPDR:   fig|264462.1.peg.956
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- PhylomeDB:   P62410
- BioCyc:   BBAC264462:BD1050-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43357; Mature: 43225

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 41-41 BINDING 123-123 BINDING 156-156 BINDING 207-207 BINDING 298-298 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANGLKGIKTVRDFELAGKVVFLRLDLNVPMENGKITDENRITASLPTIKYCMEQGAKLV
CCCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCEECCCCCEEECCHHHHHHHHCCCCEE
MASHLGRPKTKDDTEFSLEPVAKRLQDLLNAEVILVEEPDSDAPKHLLPSLKPHQLILLE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCEEEEEE
NVRFEEGETKDSVEFAQKIANYSDIYINDAFGASHRAHATIHALPSVMKDKGIGFLIEKE
CCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHH
ITMLDSLLQNPKRPYIAVMGGAKVSDKIAVIERLMDVVDGFIVGGAMAYTFLKAQGLPVG
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSLVENDKLKYAKEMIERIEARNKTILLPVDHVATKGITDTAHAHVTNDVAIAEDELGVD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCHHEECCCEEEECCCCCCC
IGPKSIKNFSAALREAGTIFWNGPMGIFENPAFAKGTFGVAQAIADSEAIKIVGGGDSAA
CCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC
AAEASGFAGKMTHISTGGGASLEYLQGDKLPGLEILRTRIR
HHHCCCCCCCEEEEECCCCCEEHEECCCCCCCHHHHHHHCC
>Mature Secondary Structure 
ANGLKGIKTVRDFELAGKVVFLRLDLNVPMENGKITDENRITASLPTIKYCMEQGAKLV
CCCCCHHHHHHHHHHCCEEEEEEEECCCCCCCCEECCCCCEEECCHHHHHHHHCCCCEE
MASHLGRPKTKDDTEFSLEPVAKRLQDLLNAEVILVEEPDSDAPKHLLPSLKPHQLILLE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCEEEEEE
NVRFEEGETKDSVEFAQKIANYSDIYINDAFGASHRAHATIHALPSVMKDKGIGFLIEKE
CCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHH
ITMLDSLLQNPKRPYIAVMGGAKVSDKIAVIERLMDVVDGFIVGGAMAYTFLKAQGLPVG
HHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KSLVENDKLKYAKEMIERIEARNKTILLPVDHVATKGITDTAHAHVTNDVAIAEDELGVD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCHHEECCCEEEECCCCCCC
IGPKSIKNFSAALREAGTIFWNGPMGIFENPAFAKGTFGVAQAIADSEAIKIVGGGDSAA
CCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCC
AAEASGFAGKMTHISTGGGASLEYLQGDKLPGLEILRTRIR
HHHCCCCCCCEEEEECCCCCEEHEECCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA