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The map label for this gene is holB [H]

Identifier: 42522598

GI number: 42522598

Start: 985644

End: 986588

Strand: Direct

Name: holB [H]

Synonym: Bd1041

Alternate gene names: 42522598

Gene position: 985644-986588 (Clockwise)

Preceding gene: 42522597

Following gene: 42522599

Centisome position: 26.05

GC content: 52.28

Gene sequence:

>945_bases
ATGGCCCGGATGCTGGATTTCGTCTTAGGACATCAGGAAACAATCAAAAAAATGGTCGAGTCTTTCGAAAACGGAAAGCC
CGGTCAGACCTTTTTGTTTGTGGGTCCGGGCGGCATCGGCAAAAAACTGACAGCGATGGGGCTGGCGCAGGCTCTGTTGT
GTCCTTCCAGCCCGCGGGGCTGTGGTAAATGCCCTTCTTGTTTCCGCATTTCCCAGGGCTCGCACGAAGGTCTTAAGTTG
ATTGCCCCCAATGGGGCCAACATCAAAATGGAACAAGCCAAAGAGGTTCTGGAATTCCTCAGTTTAAAAAGTCTGACAGG
CAACCGTGTGATCGTCATTGATCAGGCACAAACCCTGAATCCGCAGGCGGCCAATTCTTTGTTAAAAACTTTGGAGGAAC
CGCCAGAGGGTACGTTCTTCTTTTTAATTGCTCCCAGTGTGGCGGGGATTCTGCCGACCATTCGGTCCCGGTCCCGCATT
GTGCAGTTCCGTCCGTTGACTCAGGAGGATCTGGGAAAACGCGTGAAAGCGCCGGCCTGGGCGTTGAAGGCCGCAGGTGG
AAGTTTTGAAAAGCTGGCTCAGTTGCAGGATGGTCCCGAGCAGGAAGTGCGCGAAAAGGCGGTGGAGCTTCTGACTTTGT
TCATTCAGGATGCTGACTTCCTGCTTAATGAACTGTGGAGAAACGAGTTCAAAGACCGCGCTCAAGGGCAGCGCATGATC
TCCTATTGGATTGGCTTCTTAAAGGATGCCATTTGTCTGCAAGAGGGTGCTAAAACTCAGATTGTGAATCTGGATCAGGC
GCCGCTGATCAAGGTCCTGGCAGAGCTGGAACGCCCACGAATTCTGAATTTGATTCAGAAAGCGCTGCAAGTGGAGCAGG
CCTTTGCCGCCAATCGGGATCCTCAGCTGATCATCGAAGAATTCTACGTCACGTCCAAGGCTTAA

Upstream 100 bases:

>100_bases
GCTGCGTGGATCGTTCTGGATGCGCGTGAAACCCCGGAAGTACTATTCAAACAGCTGTTGCAGTCTTTGACTGAACGAAA
AGTTTTGTAAGGACGTAGCA

Downstream 100 bases:

>100_bases
AGCGCATTGTCACCTTCCGTGACGGCTGTTATACTTGCCCTATGGAATGGATCGATATTCACGCACATTTGAATATGCTC
GAAGAGGGTGTTGAAGCCGC

Product: DNA polymerase III delta' subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MARMLDFVLGHQETIKKMVESFENGKPGQTFLFVGPGGIGKKLTAMGLAQALLCPSSPRGCGKCPSCFRISQGSHEGLKL
IAPNGANIKMEQAKEVLEFLSLKSLTGNRVIVIDQAQTLNPQAANSLLKTLEEPPEGTFFFLIAPSVAGILPTIRSRSRI
VQFRPLTQEDLGKRVKAPAWALKAAGGSFEKLAQLQDGPEQEVREKAVELLTLFIQDADFLLNELWRNEFKDRAQGQRMI
SYWIGFLKDAICLQEGAKTQIVNLDQAPLIKVLAELERPRILNLIQKALQVEQAFAANRDPQLIIEEFYVTSKA

Sequences:

>Translated_314_residues
MARMLDFVLGHQETIKKMVESFENGKPGQTFLFVGPGGIGKKLTAMGLAQALLCPSSPRGCGKCPSCFRISQGSHEGLKL
IAPNGANIKMEQAKEVLEFLSLKSLTGNRVIVIDQAQTLNPQAANSLLKTLEEPPEGTFFFLIAPSVAGILPTIRSRSRI
VQFRPLTQEDLGKRVKAPAWALKAAGGSFEKLAQLQDGPEQEVREKAVELLTLFIQDADFLLNELWRNEFKDRAQGQRMI
SYWIGFLKDAICLQEGAKTQIVNLDQAPLIKVLAELERPRILNLIQKALQVEQAFAANRDPQLIIEEFYVTSKA
>Mature_313_residues
ARMLDFVLGHQETIKKMVESFENGKPGQTFLFVGPGGIGKKLTAMGLAQALLCPSSPRGCGKCPSCFRISQGSHEGLKLI
APNGANIKMEQAKEVLEFLSLKSLTGNRVIVIDQAQTLNPQAANSLLKTLEEPPEGTFFFLIAPSVAGILPTIRSRSRIV
QFRPLTQEDLGKRVKAPAWALKAAGGSFEKLAQLQDGPEQEVREKAVELLTLFIQDADFLLNELWRNEFKDRAQGQRMIS
YWIGFLKDAICLQEGAKTQIVNLDQAPLIKVLAELERPRILNLIQKALQVEQAFAANRDPQLIIEEFYVTSKA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=287, Percent_Identity=29.616724738676, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786676, Length=207, Percent_Identity=28.9855072463768, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34758; Mature: 34627

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMLDFVLGHQETIKKMVESFENGKPGQTFLFVGPGGIGKKLTAMGLAQALLCPSSPRG
CHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
CGKCPSCFRISQGSHEGLKLIAPNGANIKMEQAKEVLEFLSLKSLTGNRVIVIDQAQTLN
CCCCCCHHEECCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
PQAANSLLKTLEEPPEGTFFFLIAPSVAGILPTIRSRSRIVQFRPLTQEDLGKRVKAPAW
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCHH
ALKAAGGSFEKLAQLQDGPEQEVREKAVELLTLFIQDADFLLNELWRNEFKDRAQGQRMI
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYWIGFLKDAICLQEGAKTQIVNLDQAPLIKVLAELERPRILNLIQKALQVEQAFAANRD
HHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
PQLIIEEFYVTSKA
HHHHHHHHHHHCCC
>Mature Secondary Structure 
ARMLDFVLGHQETIKKMVESFENGKPGQTFLFVGPGGIGKKLTAMGLAQALLCPSSPRG
HHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
CGKCPSCFRISQGSHEGLKLIAPNGANIKMEQAKEVLEFLSLKSLTGNRVIVIDQAQTLN
CCCCCCHHEECCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
PQAANSLLKTLEEPPEGTFFFLIAPSVAGILPTIRSRSRIVQFRPLTQEDLGKRVKAPAW
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCCHH
ALKAAGGSFEKLAQLQDGPEQEVREKAVELLTLFIQDADFLLNELWRNEFKDRAQGQRMI
HHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYWIGFLKDAICLQEGAKTQIVNLDQAPLIKVLAELERPRILNLIQKALQVEQAFAANRD
HHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
PQLIIEEFYVTSKA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]