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The map label for this gene is usf [H]

Identifier: 42522544

GI number: 42522544

Start: 927960

End: 928748

Strand: Direct

Name: usf [H]

Synonym: Bd0982

Alternate gene names: 42522544

Gene position: 927960-928748 (Clockwise)

Preceding gene: 42522539

Following gene: 42522545

Centisome position: 24.53

GC content: 52.72

Gene sequence:

>789_bases
ATGATGAAGAATTTAGCAGCAGCCCTTTTGGTGTGGGGGGCATTCATGCAAGCTCATGCTGAAGTAAAAACTGAAATGGT
CGAGTACAAAGAAGGCAAGACGGTTTTGGAAGGTTTTCTGGCTCAGGATGATTCCTTAAAAGGTCCGCGTCCGGCGATCA
TCATTGTTCACCAGTGGATGGGGTTGGGTGAACATGAAAAAGCCAGCGCCCAGCGTTTGGCGGAAAAAGGTTACGTGGTA
CTGGCTGCCGACATCTATGGCAAAGGGGTCCGTCCCGGATCCCCGGCAGAGGCCGGCAAACTTGCCGGCACATACAAAGA
GGACGTGAAGCTTTACCGGGCGCGTGAAAAAGCGGCCTTTGATTATCTGAAGAAAAATAAAAATGTCGATGCCAAGCAGA
TAGTGATCATGGGGTATTGCTTTGGCGGTACCGGAGCCCTGGAGGCGGCTCGTGCAGGTTTGCCTGTGGTGGGGGCGGTC
AGCATCCATGGTGGTCTGGCCAGCAAGAATCCCAAGGATGTGAAGAACATCAAATCCAAGGTGCTGGTCTTGCATGGAGC
TATTGATCCCTACGTGCCACCAGCGGAAGTCGATGGCTTCATGAAAGAAATGAACGAAGCCAAAGTGGACTATCAGTTTG
TGGCGTATTCCGGAGCGGTTCATGCCTTCACTCAGAAAGATGCGGGCAATGATCCATCCAAGGGGCACGCCTACAACCCC
GTCGCGGAGAAACGTTCCTGGGCGGCTCTGGAGGCCTTTCTGAATGAAGTGGCACCAGTTGCAAGGTAA

Upstream 100 bases:

>100_bases
AAATCAGGAATTTGTGAGTCATCAGGACCCTGATGAGGGTTTTTTGACCTTTCGCCGGGCCCTTCATAGACTTTGGTAAA
GCTTAAAAGGAGTACTAAAG

Downstream 100 bases:

>100_bases
TGCGAATTGAGCTGTCTAGAGTCTGAACAATCGTGAAAATATGGCAAGGGTGGTGACGGGGAATGGTCCTGTCGCCGCCC
TTGTGTTAAAGACTTCGCTT

Product: dienelactone hydrolase family protein

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MMKNLAAALLVWGAFMQAHAEVKTEMVEYKEGKTVLEGFLAQDDSLKGPRPAIIIVHQWMGLGEHEKASAQRLAEKGYVV
LAADIYGKGVRPGSPAEAGKLAGTYKEDVKLYRAREKAAFDYLKKNKNVDAKQIVIMGYCFGGTGALEAARAGLPVVGAV
SIHGGLASKNPKDVKNIKSKVLVLHGAIDPYVPPAEVDGFMKEMNEAKVDYQFVAYSGAVHAFTQKDAGNDPSKGHAYNP
VAEKRSWAALEAFLNEVAPVAR

Sequences:

>Translated_262_residues
MMKNLAAALLVWGAFMQAHAEVKTEMVEYKEGKTVLEGFLAQDDSLKGPRPAIIIVHQWMGLGEHEKASAQRLAEKGYVV
LAADIYGKGVRPGSPAEAGKLAGTYKEDVKLYRAREKAAFDYLKKNKNVDAKQIVIMGYCFGGTGALEAARAGLPVVGAV
SIHGGLASKNPKDVKNIKSKVLVLHGAIDPYVPPAEVDGFMKEMNEAKVDYQFVAYSGAVHAFTQKDAGNDPSKGHAYNP
VAEKRSWAALEAFLNEVAPVAR
>Mature_262_residues
MMKNLAAALLVWGAFMQAHAEVKTEMVEYKEGKTVLEGFLAQDDSLKGPRPAIIIVHQWMGLGEHEKASAQRLAEKGYVV
LAADIYGKGVRPGSPAEAGKLAGTYKEDVKLYRAREKAAFDYLKKNKNVDAKQIVIMGYCFGGTGALEAARAGLPVVGAV
SIHGGLASKNPKDVKNIKSKVLVLHGAIDPYVPPAEVDGFMKEMNEAKVDYQFVAYSGAVHAFTQKDAGNDPSKGHAYNP
VAEKRSWAALEAFLNEVAPVAR

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=201, Percent_Identity=24.3781094527363, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536419, Length=242, Percent_Identity=37.603305785124, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17532655, Length=243, Percent_Identity=36.6255144032922, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17536415, Length=238, Percent_Identity=33.6134453781513, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17532653, Length=220, Percent_Identity=33.6363636363636, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI193204305, Length=212, Percent_Identity=32.5471698113208, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17534829, Length=147, Percent_Identity=34.0136054421769, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 28244; Mature: 28244

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKNLAAALLVWGAFMQAHAEVKTEMVEYKEGKTVLEGFLAQDDSLKGPRPAIIIVHQWM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEECCC
GLGEHEKASAQRLAEKGYVVLAADIYGKGVRPGSPAEAGKLAGTYKEDVKLYRAREKAAF
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
DYLKKNKNVDAKQIVIMGYCFGGTGALEAARAGLPVVGAVSIHGGLASKNPKDVKNIKSK
HHHHCCCCCCHHEEEEEEEEECCCCHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHH
VLVLHGAIDPYVPPAEVDGFMKEMNEAKVDYQFVAYSGAVHAFTQKDAGNDPSKGHAYNP
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCCCCCCCCCC
VAEKRSWAALEAFLNEVAPVAR
HHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMKNLAAALLVWGAFMQAHAEVKTEMVEYKEGKTVLEGFLAQDDSLKGPRPAIIIVHQWM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEECCC
GLGEHEKASAQRLAEKGYVVLAADIYGKGVRPGSPAEAGKLAGTYKEDVKLYRAREKAAF
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
DYLKKNKNVDAKQIVIMGYCFGGTGALEAARAGLPVVGAVSIHGGLASKNPKDVKNIKSK
HHHHCCCCCCHHEEEEEEEEECCCCHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHH
VLVLHGAIDPYVPPAEVDGFMKEMNEAKVDYQFVAYSGAVHAFTQKDAGNDPSKGHAYNP
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCCCCCCCCCC
VAEKRSWAALEAFLNEVAPVAR
HHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]