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The map label for this gene is eno

Identifier: 42522372

GI number: 42522372

Start: 745744

End: 747012

Strand: Direct

Name: eno

Synonym: Bd0796

Alternate gene names: 42522372

Gene position: 745744-747012 (Clockwise)

Preceding gene: 42522371

Following gene: 42522373

Centisome position: 19.71

GC content: 50.2

Gene sequence:

>1269_bases
ATGTCTGAAATCATCAGTGTCATCTCTCGTGAAATCCTGGACAGCCGTGGAAACCCGACTGTAGAGGTTGAAGTTACAAC
AGCTGACGGCAATATGGGCCGCGCAGCAGTTCCATCTGGTGCCTCCACAGGTGCTCACGAAGCTTGCGAGCTTCGCGATG
GCGACAAAAACCGTTTCCTGGGTAAAGGCGTTTACAAAGCTGTCGACAATGTTCGTGAAAAAATCGCTCCGGAAATCGTG
GGCCTTCAGGCAACTGAACAGGTTTACATCGATAAAATCCTGCGCGACATCGACGGCACTGAAAATAAATCCAACTTGGG
CGCAAATGCGATCCTGGGTGTTTCTTTGGCAGTTGCCAAAGCGGCAGCTAAAGACTGCCGTTTGCCGCTTTACCGTTACG
TTGGTGGCTCTCAGGCTTCTCGCTTGCCGGTTCCATTGATGAACGTTCTGAACGGTGGCGCGCACGCGAACAACGGTTTG
GATATTCAGGAATTCATGATCGTTCCAACTGTGAACAACTCATACGCTGAGTCCCTGCGTGCAGGTACTGAGATCTTCCA
CACTCTGAAAAAGATCCTGGCGAAAAAAGGTCTTTCCACAGCAGTAGGTGACGAAGGTGGCTTCGCGCCGAAATTGGGTT
CCAATCAGGAAGCTCTTGATTTGCTGATGAATGCGATTGTGGATGCTGGTTATGATCCAGGTCAGAACGTGTTCCTGGCG
TTGGACGTGGCTGCAACTGAAATGTTCAAAGAAGGCAAATACGAATGGCAGGGTGGTCACATCAGCCCGACCGAACTTCT
TGGCATCTACAAATCCTGGGCAGAGAAATATCCTCTTGTTTCCATTGAAGACGGCTTCGCGGAAGACGACTGGGATTCCT
GGGTTCAGTCCACAGCTCAAATGGGCTCCACTATGCAGTTGATTGGTGATGATTTGTTTGTGACCAATCCAAAACGTCTG
CGCATGGGTCTTGAGAAAAAAGCGGGTAACGCTTTGTTGGTGAAAGTAAACCAAATCGGTACTTTGACTGAAACTTACGA
AGCCGTGAACCTGGCTCAACGTAACAAATTCCGCACAATCATGTCCCACCGCTCTGGTGAGACGGAAGATGTGACGATTG
CAGATCTGGCTGTAGGCCTGAACTGCCATCAGATCAAAACAGGCAGCTTGTGCCGTGGTGAAAGAACCGCGAAGTACAAC
CAGCTTCTGAGAATCGAAGAAGACTTGGGCGGTATGGGCCTATACTGGGACAAAGCCGCCTTCCGATAG

Upstream 100 bases:

>100_bases
TGGGAGTTCGTAAAAACCCAGTACCCGCGCACGGTCTTTGAAAGCATGGATCTGGTTAAGAAAACGATGAATAAAAACGC
CGACAAAGAAGGAAATCAAC

Downstream 100 bases:

>100_bases
GCGGCCGCTGAAAAGGGTCCGACTGACTTCGTTGTGCTGCGGGCATCCTGCCCTTGCCCTTCGGGCTGACGCGCAAGAAT
AGCTTCCTGCTATTCTTGTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MSEIISVISREILDSRGNPTVEVEVTTADGNMGRAAVPSGASTGAHEACELRDGDKNRFLGKGVYKAVDNVREKIAPEIV
GLQATEQVYIDKILRDIDGTENKSNLGANAILGVSLAVAKAAAKDCRLPLYRYVGGSQASRLPVPLMNVLNGGAHANNGL
DIQEFMIVPTVNNSYAESLRAGTEIFHTLKKILAKKGLSTAVGDEGGFAPKLGSNQEALDLLMNAIVDAGYDPGQNVFLA
LDVAATEMFKEGKYEWQGGHISPTELLGIYKSWAEKYPLVSIEDGFAEDDWDSWVQSTAQMGSTMQLIGDDLFVTNPKRL
RMGLEKKAGNALLVKVNQIGTLTETYEAVNLAQRNKFRTIMSHRSGETEDVTIADLAVGLNCHQIKTGSLCRGERTAKYN
QLLRIEEDLGGMGLYWDKAAFR

Sequences:

>Translated_422_residues
MSEIISVISREILDSRGNPTVEVEVTTADGNMGRAAVPSGASTGAHEACELRDGDKNRFLGKGVYKAVDNVREKIAPEIV
GLQATEQVYIDKILRDIDGTENKSNLGANAILGVSLAVAKAAAKDCRLPLYRYVGGSQASRLPVPLMNVLNGGAHANNGL
DIQEFMIVPTVNNSYAESLRAGTEIFHTLKKILAKKGLSTAVGDEGGFAPKLGSNQEALDLLMNAIVDAGYDPGQNVFLA
LDVAATEMFKEGKYEWQGGHISPTELLGIYKSWAEKYPLVSIEDGFAEDDWDSWVQSTAQMGSTMQLIGDDLFVTNPKRL
RMGLEKKAGNALLVKVNQIGTLTETYEAVNLAQRNKFRTIMSHRSGETEDVTIADLAVGLNCHQIKTGSLCRGERTAKYN
QLLRIEEDLGGMGLYWDKAAFR
>Mature_421_residues
SEIISVISREILDSRGNPTVEVEVTTADGNMGRAAVPSGASTGAHEACELRDGDKNRFLGKGVYKAVDNVREKIAPEIVG
LQATEQVYIDKILRDIDGTENKSNLGANAILGVSLAVAKAAAKDCRLPLYRYVGGSQASRLPVPLMNVLNGGAHANNGLD
IQEFMIVPTVNNSYAESLRAGTEIFHTLKKILAKKGLSTAVGDEGGFAPKLGSNQEALDLLMNAIVDAGYDPGQNVFLAL
DVAATEMFKEGKYEWQGGHISPTELLGIYKSWAEKYPLVSIEDGFAEDDWDSWVQSTAQMGSTMQLIGDDLFVTNPKRLR
MGLEKKAGNALLVKVNQIGTLTETYEAVNLAQRNKFRTIMSHRSGETEDVTIADLAVGLNCHQIKTGSLCRGERTAKYNQ
LLRIEEDLGGMGLYWDKAAFR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=421, Percent_Identity=53.6817102137767, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI5803011, Length=421, Percent_Identity=53.4441805225653, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=53.6130536130536, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=53.6130536130536, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=49.6487119437939, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=350, Percent_Identity=28.2857142857143, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI169201757, Length=350, Percent_Identity=28.2857142857143, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI239744207, Length=350, Percent_Identity=28.2857142857143, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI310129182, Length=140, Percent_Identity=33.5714285714286, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310110045, Length=140, Percent_Identity=33.5714285714286, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI310120572, Length=140, Percent_Identity=33.5714285714286, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789141, Length=417, Percent_Identity=54.6762589928058, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71995829, Length=427, Percent_Identity=54.3325526932084, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17536383, Length=427, Percent_Identity=54.3325526932084, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32563855, Length=187, Percent_Identity=50.8021390374332, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=429, Percent_Identity=49.6503496503497, Blast_Score=388, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=430, Percent_Identity=49.7674418604651, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=430, Percent_Identity=49.7674418604651, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=430, Percent_Identity=49.7674418604651, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=429, Percent_Identity=49.6503496503497, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=50.6912442396313, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=50.6912442396313, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=50.6912442396313, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=50.6912442396313, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=50.6912442396313, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=50.6912442396313, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BDEBA (Q6MPQ2)

Other databases:

- EMBL:   BX842648
- RefSeq:   NP_967752.1
- ProteinModelPortal:   Q6MPQ2
- SMR:   Q6MPQ2
- GeneID:   2733629
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0796
- NMPDR:   fig|264462.1.peg.722
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q6MPQ2
- BioCyc:   BBAC264462:BD0796-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45773; Mature: 45642

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 335-335 BINDING 155-155 BINDING 164-164 BINDING 283-283 BINDING 310-310 BINDING 335-335 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIISVISREILDSRGNPTVEVEVTTADGNMGRAAVPSGASTGAHEACELRDGDKNRFL
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHH
GKGVYKAVDNVREKIAPEIVGLQATEQVYIDKILRDIDGTENKSNLGANAILGVSLAVAK
HHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AAAKDCRLPLYRYVGGSQASRLPVPLMNVLNGGAHANNGLDIQEFMIVPTVNNSYAESLR
HHHHHCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCCHHHHHHH
AGTEIFHTLKKILAKKGLSTAVGDEGGFAPKLGSNQEALDLLMNAIVDAGYDPGQNVFLA
HHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVAATEMFKEGKYEWQGGHISPTELLGIYKSWAEKYPLVSIEDGFAEDDWDSWVQSTAQ
EEHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHH
MGSTMQLIGDDLFVTNPKRLRMGLEKKAGNALLVKVNQIGTLTETYEAVNLAQRNKFRTI
HCCCHHHHCCCEEEECHHHHHHCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
MSHRSGETEDVTIADLAVGLNCHQIKTGSLCRGERTAKYNQLLRIEEDLGGMGLYWDKAA
HHCCCCCCCCEEEEHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCHHC
FR
CC
>Mature Secondary Structure 
SEIISVISREILDSRGNPTVEVEVTTADGNMGRAAVPSGASTGAHEACELRDGDKNRFL
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHH
GKGVYKAVDNVREKIAPEIVGLQATEQVYIDKILRDIDGTENKSNLGANAILGVSLAVAK
HHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
AAAKDCRLPLYRYVGGSQASRLPVPLMNVLNGGAHANNGLDIQEFMIVPTVNNSYAESLR
HHHHHCCCCHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEEECCCCHHHHHHH
AGTEIFHTLKKILAKKGLSTAVGDEGGFAPKLGSNQEALDLLMNAIVDAGYDPGQNVFLA
HHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDVAATEMFKEGKYEWQGGHISPTELLGIYKSWAEKYPLVSIEDGFAEDDWDSWVQSTAQ
EEHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHH
MGSTMQLIGDDLFVTNPKRLRMGLEKKAGNALLVKVNQIGTLTETYEAVNLAQRNKFRTI
HCCCHHHHCCCEEEECHHHHHHCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
MSHRSGETEDVTIADLAVGLNCHQIKTGSLCRGERTAKYNQLLRIEEDLGGMGLYWDKAA
HHCCCCCCCCEEEEHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCHHC
FR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA