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The map label for this gene is rpoE [H]

Identifier: 42522327

GI number: 42522327

Start: 706483

End: 707103

Strand: Direct

Name: rpoE [H]

Synonym: Bd0743

Alternate gene names: 42522327

Gene position: 706483-707103 (Clockwise)

Preceding gene: 42522326

Following gene: 42522328

Centisome position: 18.68

GC content: 48.15

Gene sequence:

>621_bases
ATGGAGAGAGATCTAAACGTAACGGATCTTGAACTGGTAGAAAAAGTGAAGTCTGGCGACAGACGCTCTTTTTCCGAACT
CGTGAAACGACATCAGAGAAGTGTGCTGCGTATGAGTTTGCGGTTCGTAAAGGACATGGACACAGCTGAGGACGTGACGC
AAGAAGCGTTCATCAAGGCTTACGAAAAGCTGAACACCTTCGAAGGTCGTTCTTCTTTCAAAAGCTGGCTGTTCCAGATC
GCCGTGAACACGGCACGCAACAAACTGCGTGAGTGGAAACGCGACACCGTGGATATCGACGATGTGCAATTGGCTGTAGA
TGCGGAGGCCGAAACTACACTCGTCCATACGGCGGTTTCTGACATACTTAAAAATGAAGTAGAGAAGCTGCCGTTCAAGC
AGAAAACAGCCCTGGTTCTTCGTGTGTACGAGGACCTGAGCTTTAACGAAATCGCTGATATTATGGAGTGCCCATACGAT
ACAGCGAAGGCGAACTACAGACATGCTCTGATGAAACTTCGTCAGACATTCGAACAGCAGGCTGAATTGAAGAACTGGAC
GGAAGAAGTAGGTGGCTTCTTCCTCGAGGTTAACCAAAGATTTGCGGAAGCAGAAGGATAA

Upstream 100 bases:

>100_bases
GTTTGGGGGCGAATTTACCGGGTGCTGGGGGGAGCAGAATGGCTCTTTCGGGAAAAAAATTCAGAACGTTAACCGAATTT
AGACAGAAACGAGTCAAAGT

Downstream 100 bases:

>100_bases
GAATCGATGGACAACGTGGACCGTATGGATCGCATGGATAAAGTGCGCAAAGGCCTTAAAGCCGCCGACGATATGGAACT
GCCCATGAACGATGATTTCT

Product: RNA polymerase sigma-E factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MERDLNVTDLELVEKVKSGDRRSFSELVKRHQRSVLRMSLRFVKDMDTAEDVTQEAFIKAYEKLNTFEGRSSFKSWLFQI
AVNTARNKLREWKRDTVDIDDVQLAVDAEAETTLVHTAVSDILKNEVEKLPFKQKTALVLRVYEDLSFNEIADIMECPYD
TAKANYRHALMKLRQTFEQQAELKNWTEEVGGFFLEVNQRFAEAEG

Sequences:

>Translated_206_residues
MERDLNVTDLELVEKVKSGDRRSFSELVKRHQRSVLRMSLRFVKDMDTAEDVTQEAFIKAYEKLNTFEGRSSFKSWLFQI
AVNTARNKLREWKRDTVDIDDVQLAVDAEAETTLVHTAVSDILKNEVEKLPFKQKTALVLRVYEDLSFNEIADIMECPYD
TAKANYRHALMKLRQTFEQQAELKNWTEEVGGFFLEVNQRFAEAEG
>Mature_206_residues
MERDLNVTDLELVEKVKSGDRRSFSELVKRHQRSVLRMSLRFVKDMDTAEDVTQEAFIKAYEKLNTFEGRSSFKSWLFQI
AVNTARNKLREWKRDTVDIDDVQLAVDAEAETTLVHTAVSDILKNEVEKLPFKQKTALVLRVYEDLSFNEIADIMECPYD
TAKANYRHALMKLRQTFEQQAELKNWTEEVGGFFLEVNQRFAEAEG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=169, Percent_Identity=37.8698224852071, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 24035; Mature: 24035

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERDLNVTDLELVEKVKSGDRRSFSELVKRHQRSVLRMSLRFVKDMDTAEDVTQEAFIKA
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YEKLNTFEGRSSFKSWLFQIAVNTARNKLREWKRDTVDIDDVQLAVDAEAETTLVHTAVS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCHHHHHHHHHH
DILKNEVEKLPFKQKTALVLRVYEDLSFNEIADIMECPYDTAKANYRHALMKLRQTFEQQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AELKNWTEEVGGFFLEVNQRFAEAEG
HHHHHHHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERDLNVTDLELVEKVKSGDRRSFSELVKRHQRSVLRMSLRFVKDMDTAEDVTQEAFIKA
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
YEKLNTFEGRSSFKSWLFQIAVNTARNKLREWKRDTVDIDDVQLAVDAEAETTLVHTAVS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCHHHHHHHHHH
DILKNEVEKLPFKQKTALVLRVYEDLSFNEIADIMECPYDTAKANYRHALMKLRQTFEQQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AELKNWTEEVGGFFLEVNQRFAEAEG
HHHHHHHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]