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The map label for this gene is ycdT [H]

Identifier: 42522326

GI number: 42522326

Start: 705077

End: 706039

Strand: Direct

Name: ycdT [H]

Synonym: Bd0742

Alternate gene names: 42522326

Gene position: 705077-706039 (Clockwise)

Preceding gene: 42522325

Following gene: 42522327

Centisome position: 18.64

GC content: 50.16

Gene sequence:

>963_bases
GTGACGGGGGGAAGTTTTATACTTTTCCCTATGGCTCACAACGATGACAACTCAGACAACTTAGAAAAAACCAGTATTGT
TGCCAGCGACACTTTCAAGGGTCGTCTTAAAGAGGCGGATGATGTTCCGCCGGCAATTGTTGTTTTGATCGGCCCTCCGG
GCTACGTCGGTAAACAATATCCTATCACGGCCAGTGATATCGTCATCGGCCGCTCTGTGGAAAGCCAAGTCTACATTGAT
GATAAAAGTTTGAGCCGCTCTCACGCAAAGTTCGCTGTCAATGGCAGTGAAGTGTCCGTGATCGATTTGGGTTCCACCAA
TAAAACCATCGTGAATGGTCAGGTGATCCCACCCCTGGCATCGTGCCTTTTGAAAAACAATGACCAGATCAAAACCGGCA
ACGTGATCTTCAAGTTCCTTGAAAAAGGCAGCATTGAAGCCATGACCAATGCGGCGATGTACGATCGTGCGCAGAAGGAT
GCGTTGACGGGTGCTCATTCTAAGGGTGCGCTTTTGGACAAGGGACCTGAGGCGATGAAACGCGCTGAAGTTCTGAACGA
GCCCTTCAGTCTTGTGACTTTCGATATCGATCACTTTAAAAAGATCAATGACAGCTACGGGCATCCGGGCGGTGACTATG
TTCTGAAAGAACTGTGCCGTATCGTGATCACCAAGCTGATCCGCTCCAACGACTTCTTTGCCCGTTACGGTGGTGAAGAG
TTTGTGCTGCTGCTTTCCGGTTCACCAAGCAAAACAGCTGGCGAAGTGGGCGAGCGTATTCGTCAGACGATTGAAGCCCA
TGACTTTACATTTGAAGGCAAGAAGATCCCGGTGACCATCTCCGTGGGTGTTGCCACCAAGCTTCCAAATGAAACCGAAT
GGACTCAAGTGTACGACCGTGCCGACAAGGCGCTGTACCAGTCCAAACAAGGTGGCCGTAACCGTACGACCATCGTCGCT
TAA

Upstream 100 bases:

>100_bases
GGGCTTGGAGATTACCTGGAAATCGACGCCTTCAATCAGCCGGGTGTCGAGCTGGGCAAGAGACTAGCGAAAGAGAAATT
GAAGAAAGCTTGATTGTCAG

Downstream 100 bases:

>100_bases
TTTTAGTTTTTATCCCGGGGTTGGCTTCTTACAAGACTTTACTGAAGACACTCCAAACCTGGGATTCTTAAAGCCGCAGC
CTAAAAAGTTGCGGCTTTTT

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTGGSFILFPMAHNDDNSDNLEKTSIVASDTFKGRLKEADDVPPAIVVLIGPPGYVGKQYPITASDIVIGRSVESQVYID
DKSLSRSHAKFAVNGSEVSVIDLGSTNKTIVNGQVIPPLASCLLKNNDQIKTGNVIFKFLEKGSIEAMTNAAMYDRAQKD
ALTGAHSKGALLDKGPEAMKRAEVLNEPFSLVTFDIDHFKKINDSYGHPGGDYVLKELCRIVITKLIRSNDFFARYGGEE
FVLLLSGSPSKTAGEVGERIRQTIEAHDFTFEGKKIPVTISVGVATKLPNETEWTQVYDRADKALYQSKQGGRNRTTIVA

Sequences:

>Translated_320_residues
MTGGSFILFPMAHNDDNSDNLEKTSIVASDTFKGRLKEADDVPPAIVVLIGPPGYVGKQYPITASDIVIGRSVESQVYID
DKSLSRSHAKFAVNGSEVSVIDLGSTNKTIVNGQVIPPLASCLLKNNDQIKTGNVIFKFLEKGSIEAMTNAAMYDRAQKD
ALTGAHSKGALLDKGPEAMKRAEVLNEPFSLVTFDIDHFKKINDSYGHPGGDYVLKELCRIVITKLIRSNDFFARYGGEE
FVLLLSGSPSKTAGEVGERIRQTIEAHDFTFEGKKIPVTISVGVATKLPNETEWTQVYDRADKALYQSKQGGRNRTTIVA
>Mature_319_residues
TGGSFILFPMAHNDDNSDNLEKTSIVASDTFKGRLKEADDVPPAIVVLIGPPGYVGKQYPITASDIVIGRSVESQVYIDD
KSLSRSHAKFAVNGSEVSVIDLGSTNKTIVNGQVIPPLASCLLKNNDQIKTGNVIFKFLEKGSIEAMTNAAMYDRAQKDA
LTGAHSKGALLDKGPEAMKRAEVLNEPFSLVTFDIDHFKKINDSYGHPGGDYVLKELCRIVITKLIRSNDFFARYGGEEF
VLLLSGSPSKTAGEVGERIRQTIEAHDFTFEGKKIPVTISVGVATKLPNETEWTQVYDRADKALYQSKQGGRNRTTIVA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=37.1951219512195, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87082007, Length=163, Percent_Identity=36.8098159509202, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787816, Length=176, Percent_Identity=35.2272727272727, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=33.9506172839506, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=167, Percent_Identity=35.3293413173653, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=33.1360946745562, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=37.5, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693134, Length=132, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=31.7073170731707, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081974, Length=160, Percent_Identity=30.625, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87081977, Length=177, Percent_Identity=29.3785310734463, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34889; Mature: 34758

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50006 FHA_DOMAIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGSFILFPMAHNDDNSDNLEKTSIVASDTFKGRLKEADDVPPAIVVLIGPPGYVGKQY
CCCCCEEEEEEECCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PITASDIVIGRSVESQVYIDDKSLSRSHAKFAVNGSEVSVIDLGSTNKTIVNGQVIPPLA
CCCHHHEEEECCCCCEEEEECCCCCCCCCEEEECCCEEEEEEECCCCCEEECCEECHHHH
SCLLKNNDQIKTGNVIFKFLEKGSIEAMTNAAMYDRAQKDALTGAHSKGALLDKGPEAMK
HHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RAEVLNEPFSLVTFDIDHFKKINDSYGHPGGDYVLKELCRIVITKLIRSNDFFARYGGEE
HHHHHCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCE
FVLLLSGSPSKTAGEVGERIRQTIEAHDFTFEGKKIPVTISVGVATKLPNETEWTQVYDR
EEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEECCCCCCHHHHHHHH
ADKALYQSKQGGRNRTTIVA
HHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure 
TGGSFILFPMAHNDDNSDNLEKTSIVASDTFKGRLKEADDVPPAIVVLIGPPGYVGKQY
CCCCEEEEEEECCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PITASDIVIGRSVESQVYIDDKSLSRSHAKFAVNGSEVSVIDLGSTNKTIVNGQVIPPLA
CCCHHHEEEECCCCCEEEEECCCCCCCCCEEEECCCEEEEEEECCCCCEEECCEECHHHH
SCLLKNNDQIKTGNVIFKFLEKGSIEAMTNAAMYDRAQKDALTGAHSKGALLDKGPEAMK
HHHHCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
RAEVLNEPFSLVTFDIDHFKKINDSYGHPGGDYVLKELCRIVITKLIRSNDFFARYGGEE
HHHHHCCCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCE
FVLLLSGSPSKTAGEVGERIRQTIEAHDFTFEGKKIPVTISVGVATKLPNETEWTQVYDR
EEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEECCCCCCHHHHHHHH
ADKALYQSKQGGRNRTTIVA
HHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]