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The map label for this gene is lipA [H]

Identifier: 42522251

GI number: 42522251

Start: 614735

End: 615751

Strand: Direct

Name: lipA [H]

Synonym: Bd0664

Alternate gene names: 42522251

Gene position: 614735-615751 (Clockwise)

Preceding gene: 42522250

Following gene: 42522252

Centisome position: 16.25

GC content: 51.72

Gene sequence:

>1017_bases
ATGGGAAAAAAGACTCTGCTGGTCCTGGTTGTCCTGGTTGCTGTGCTGGCTGGAGCCAGCTGGATCACCATGCAAATAAC
ACCATGGCCGAAGGCCATGGTGATTCGCTGGCTCTTTGATGAAGGCGCCGAAGAGGCCTCTGCGGTGCTGGCCAAGCATC
TTCCCACCAATATAAGTCAAAGACTTGATGTCTCTTATGATCCCTTCGACAAAGACGCCTTTGTGGATTTCTATTTTCCG
ACTGAAGCAGAAGCCCAGGGATCATTGCTGCCTTTGATCGTCTGGGTTCATGGGGGCGCCTGGATTTCGGGGGACAAGGC
GGATGTTGGCAACTATGCCCGGATCCTGGCCTCGCGCGGATTTGTGGTGGCCTCTGTCGGATATTCAATAGCACCCGAGT
TTCAATATCCCACACCTCTTCGCCAGATTCTACTGGCGCTTTCTTTTATTGACGAAAATGCCTCTGACTATCATGCCGAC
TCCACCCGGGTCTTTTTGGCCGGAGATTCTGCCGGCGCCCAATTGGTCGCACAAACGGCCAACATTCTGACATCTCCGAC
GTACGCCGAGCAAATGGAAATGCTGCCGACTTTGGCAAAAGAAAAACTGAAAGGTCTGCTGCTGTACTGTGGTGCCTATG
ATCCCACGCTGGTCAACTTTGAAGGTTCCTTTGGTGGATTTTTGAATACTGTGTTTTGGTCCTACTTCGGGACCAAGGAC
TTTCTCAAGGATGAACGGATGAAAGAGTTCTCGGTCCTGCAGAATATGACCCGGGATTTTCCGGCGATGTTTGTCTCTGC
CGGAAATGCAGACCCGCTGCTGGGGCAATCCAGAGCCCTGGCTGAAACCGCCACCCGTTTGGGAGTTCCGGTGCAGACAC
TGTTCTTTGCGCAGGATCATGCCCCGGGTTTAGGTCATGAATATCAGTTCAATCTGGATACGGCGGCCGGTCAGGAAGCC
TTGCAACGATCTGTTGAATTTATGCAAAACCAATTAAAAAAGGAGACGTCTTTATGA

Upstream 100 bases:

>100_bases
CACAGATGAAGATCCGCGCGCCAAAGTGGGCACCACCGACGGCTTCTAAGCGGTTGAAACACAAATGGACCCAGTATAGT
CGCCCTTATATTGTGAAGGG

Downstream 100 bases:

>100_bases
AGGTGTTATCCGTTTTTTTAAGTCTGCTCTTGGCAGTTCCCGCCTATCCGTGCACCCGCATTTTGTGGGACGGGAAAAAC
CAGGATGTTATTGTGGGTCG

Product: lipase LipA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MGKKTLLVLVVLVAVLAGASWITMQITPWPKAMVIRWLFDEGAEEASAVLAKHLPTNISQRLDVSYDPFDKDAFVDFYFP
TEAEAQGSLLPLIVWVHGGAWISGDKADVGNYARILASRGFVVASVGYSIAPEFQYPTPLRQILLALSFIDENASDYHAD
STRVFLAGDSAGAQLVAQTANILTSPTYAEQMEMLPTLAKEKLKGLLLYCGAYDPTLVNFEGSFGGFLNTVFWSYFGTKD
FLKDERMKEFSVLQNMTRDFPAMFVSAGNADPLLGQSRALAETATRLGVPVQTLFFAQDHAPGLGHEYQFNLDTAAGQEA
LQRSVEFMQNQLKKETSL

Sequences:

>Translated_338_residues
MGKKTLLVLVVLVAVLAGASWITMQITPWPKAMVIRWLFDEGAEEASAVLAKHLPTNISQRLDVSYDPFDKDAFVDFYFP
TEAEAQGSLLPLIVWVHGGAWISGDKADVGNYARILASRGFVVASVGYSIAPEFQYPTPLRQILLALSFIDENASDYHAD
STRVFLAGDSAGAQLVAQTANILTSPTYAEQMEMLPTLAKEKLKGLLLYCGAYDPTLVNFEGSFGGFLNTVFWSYFGTKD
FLKDERMKEFSVLQNMTRDFPAMFVSAGNADPLLGQSRALAETATRLGVPVQTLFFAQDHAPGLGHEYQFNLDTAAGQEA
LQRSVEFMQNQLKKETSL
>Mature_337_residues
GKKTLLVLVVLVAVLAGASWITMQITPWPKAMVIRWLFDEGAEEASAVLAKHLPTNISQRLDVSYDPFDKDAFVDFYFPT
EAEAQGSLLPLIVWVHGGAWISGDKADVGNYARILASRGFVVASVGYSIAPEFQYPTPLRQILLALSFIDENASDYHADS
TRVFLAGDSAGAQLVAQTANILTSPTYAEQMEMLPTLAKEKLKGLLLYCGAYDPTLVNFEGSFGGFLNTVFWSYFGTKDF
LKDERMKEFSVLQNMTRDFPAMFVSAGNADPLLGQSRALAETATRLGVPVQTLFFAQDHAPGLGHEYQFNLDTAAGQEAL
QRSVEFMQNQLKKETSL

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=250, Percent_Identity=26.4, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 37177; Mature: 37046

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKTLLVLVVLVAVLAGASWITMQITPWPKAMVIRWLFDEGAEEASAVLAKHLPTNISQ
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHH
RLDVSYDPFDKDAFVDFYFPTEAEAQGSLLPLIVWVHGGAWISGDKADVGNYARILASRG
HCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCEEECCCCCCHHHHHHHHHCCC
FVVASVGYSIAPEFQYPTPLRQILLALSFIDENASDYHADSTRVFLAGDSAGAQLVAQTA
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
NILTSPTYAEQMEMLPTLAKEKLKGLLLYCGAYDPTLVNFEGSFGGFLNTVFWSYFGTKD
HHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHH
FLKDERMKEFSVLQNMTRDFPAMFVSAGNADPLLGQSRALAETATRLGVPVQTLFFAQDH
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHCCCHHHEEEECCC
APGLGHEYQFNLDTAAGQEALQRSVEFMQNQLKKETSL
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKKTLLVLVVLVAVLAGASWITMQITPWPKAMVIRWLFDEGAEEASAVLAKHLPTNISQ
CCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHH
RLDVSYDPFDKDAFVDFYFPTEAEAQGSLLPLIVWVHGGAWISGDKADVGNYARILASRG
HCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCEEECCCCCCHHHHHHHHHCCC
FVVASVGYSIAPEFQYPTPLRQILLALSFIDENASDYHADSTRVFLAGDSAGAQLVAQTA
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
NILTSPTYAEQMEMLPTLAKEKLKGLLLYCGAYDPTLVNFEGSFGGFLNTVFWSYFGTKD
HHHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHH
FLKDERMKEFSVLQNMTRDFPAMFVSAGNADPLLGQSRALAETATRLGVPVQTLFFAQDH
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHCCCHHHEEEECCC
APGLGHEYQFNLDTAAGQEALQRSVEFMQNQLKKETSL
CCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA