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The map label for this gene is rsmD [H]

Identifier: 42522213

GI number: 42522213

Start: 580688

End: 581287

Strand: Direct

Name: rsmD [H]

Synonym: Bd0622

Alternate gene names: 42522213

Gene position: 580688-581287 (Clockwise)

Preceding gene: 42522212

Following gene: 42522214

Centisome position: 15.35

GC content: 50.33

Gene sequence:

>600_bases
ATGAGAATTATTGCGGGGAAATATCGTGGACATCAGCTGGTGGCATTTGATGCCGACCACATCCGCCCCACAACGGACCG
GGTGAAAGAAACCCTGTTCAATAAATTGCAATTTGATATCGACGGCGCCCGTGTGGTGGACCTGTTCTGCGGCACCGGCA
ATCTGGGCATTGAAGCTTTGTCCCGCGGGGCGGAGTTCTGCACCTTCGTTGAAAAGAACCCCAAGTCTTTGGTCATCACG
CGCAAGAATTTTGAAAAGCTGCGTGTGCCGGCTTCGGATTACAAGATCGTCAATATGGACGTGATCGCTTATTTGAAATC
CTACGAGGGCGAACCGTTTAATATTATCTTTGCCGATCCTCCGTTTACTGAAAAGATGGCCCACTTCGTAATGGAAGCCG
CCAGTACCAGTGCAGCGTTTGGGAAAACGACCCTGCTGGCGATTGAGTCCCAGGCCAAAGAACGCATGGAAGACCGTTAC
GGCTCTGTGGTTCGTTACAGCAAAAAAGAATATGGCGATAAAATCCTAAGTCTGTTCTGCCACGAAAGTGCCTTGGAGCA
GGATGAAGAACCGGCAGCCGAAGAGGAACATCATGAGTAA

Upstream 100 bases:

>100_bases
ACAACATGGAAACCGCTCAGCAGATGGGCGCGAATCTTTTTGTGCCGAAGCCTTTTGAAGATATTTTTGCCGTTGTGAAA
ATGGCAGAGGATTTGTAGTC

Downstream 100 bases:

>100_bases
AATTGCCGTTTACCCAGGAAGCTTTGACCCCATCACAATGGGGCATGTGGATATCATCAACCGTATTTCCCCTTTGTATG
ACGAAGTCATCGTGTTGGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MRIIAGKYRGHQLVAFDADHIRPTTDRVKETLFNKLQFDIDGARVVDLFCGTGNLGIEALSRGAEFCTFVEKNPKSLVIT
RKNFEKLRVPASDYKIVNMDVIAYLKSYEGEPFNIIFADPPFTEKMAHFVMEAASTSAAFGKTTLLAIESQAKERMEDRY
GSVVRYSKKEYGDKILSLFCHESALEQDEEPAAEEEHHE

Sequences:

>Translated_199_residues
MRIIAGKYRGHQLVAFDADHIRPTTDRVKETLFNKLQFDIDGARVVDLFCGTGNLGIEALSRGAEFCTFVEKNPKSLVIT
RKNFEKLRVPASDYKIVNMDVIAYLKSYEGEPFNIIFADPPFTEKMAHFVMEAASTSAAFGKTTLLAIESQAKERMEDRY
GSVVRYSKKEYGDKILSLFCHESALEQDEEPAAEEEHHE
>Mature_199_residues
MRIIAGKYRGHQLVAFDADHIRPTTDRVKETLFNKLQFDIDGARVVDLFCGTGNLGIEALSRGAEFCTFVEKNPKSLVIT
RKNFEKLRVPASDYKIVNMDVIAYLKSYEGEPFNIIFADPPFTEKMAHFVMEAASTSAAFGKTTLLAIESQAKERMEDRY
GSVVRYSKKEYGDKILSLFCHESALEQDEEPAAEEEHHE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=190, Percent_Identity=32.6315789473684, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22551; Mature: 22551

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIAGKYRGHQLVAFDADHIRPTTDRVKETLFNKLQFDIDGARVVDLFCGTGNLGIEAL
CEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHH
SRGAEFCTFVEKNPKSLVITRKNFEKLRVPASDYKIVNMDVIAYLKSYEGEPFNIIFADP
HCCHHHHHHCCCCCCEEEEECCCHHHCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEECCC
PFTEKMAHFVMEAASTSAAFGKTTLLAIESQAKERMEDRYGSVVRYSKKEYGDKILSLFC
CHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HESALEQDEEPAAEEEHHE
HHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIIAGKYRGHQLVAFDADHIRPTTDRVKETLFNKLQFDIDGARVVDLFCGTGNLGIEAL
CEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHH
SRGAEFCTFVEKNPKSLVITRKNFEKLRVPASDYKIVNMDVIAYLKSYEGEPFNIIFADP
HCCHHHHHHCCCCCCEEEEECCCHHHCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEECCC
PFTEKMAHFVMEAASTSAAFGKTTLLAIESQAKERMEDRYGSVVRYSKKEYGDKILSLFC
CHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HESALEQDEEPAAEEEHHE
HHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]