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The map label for this gene is pleD [H]

Identifier: 42521982

GI number: 42521982

Start: 344692

End: 345723

Strand: Direct

Name: pleD [H]

Synonym: Bd0367

Alternate gene names: 42521982

Gene position: 344692-345723 (Clockwise)

Preceding gene: 42521979

Following gene: 42521983

Centisome position: 9.11

GC content: 52.13

Gene sequence:

>1032_bases
ATGAGTAGGGCAGAAGTTACACTGGTATGTAAGATGAGTTTTGAAGTAAGCCCTAAACAACCCAAAAGTCGCCGTATTCT
AGTAATCGACGACGATAAAGACAGCCTGGAGATTCTCCTGGAGCCGTTGCGCTGGGAGGGCTACGACGCGCGTGGTGTGA
CCACGGAAGCCGAAGCCCACAAGCTCATCGAATCCTGGATTCCTCACATTGTCATTTTAGACTGGATGGCGCCTTCCATG
GCGGGCTTGCGCGTATTGAAGTCGGTGCGTGAGCGTCTGTCTCATGTTTCCTGTGTGTTTGTTTCTGAAAATTCCAGCAC
TGAAGCGATCATTGAAGCTCTGGATTCCGGGGCGGATGACTATATCGTAAAGCCTTTTGTGCCGTTGGAGCTTCTGGCAC
GCATTCGTTCGCAACTGCGTATCCGTGACCTGCACGAGCAGCTGCTTTTCGCCAATGAAAAACTCAAAGAGCTTGTAGAC
ACCGATGACCTGACGGGTCTGTATAACATGCGTTCGCTGTATCAACGCCTGGACTTCGAGATGGAGCGCGGTCGTCGTTT
CCATCGTGATGTCTGTGTCGTGATGATGGATATGGACTATTTCAAAACCGTGAATGACGGTCATGACCACTTGTTCGGAA
GCTATGTGCTGTCGGAGGTGGGGAAAATCATTCGCGCCAATACCCGTAATATCGACATCCCGGCCCGTTACGGCGGGGAT
GAATTCCTGATGGTGTTGACAGAGACCAACCACGCCGGGGCGATGTATTTCTGTGAACGCTTGCGTGAAAATATTGAAAA
GACCACGTTCCGCAATGGTGAGGACTCCATGAAGCTGACGGCTTCATTGGGCTTTGCTATCACCATTCCGGGGGAAAATA
TCAGCGCCCGTGAACTGGTTCGCCGCGCCGACCATGCCCTGTATCAGGCCAAGAGGGCGGGACGCAATCAAGTGGCTCAT
TACAAGCCCGAAAGTGCGCCTGTGGTGGAAATCAAATCCGCTGTTCATAAGCGTCGTAAAGCGGCCGGCTAA

Upstream 100 bases:

>100_bases
GAGATCAGCCTCCAGTCCTTCTCCCGGCGGGGCAGGATTAAAGTTTGGTAGTCTGGTCTTATGATTAGAAAATTGTCTAA
ATAATTCCCGGAATTAACCG

Downstream 100 bases:

>100_bases
TTATTCGTTGACCTGCCTCGCTCTATCTAAGAAAACGCTCCTATATGCAAAATGCTGTGTCTTCTTCTGACAAGATCCGT
AATCGTGCCGCCTGGGTTTC

Product: two component response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSRAEVTLVCKMSFEVSPKQPKSRRILVIDDDKDSLEILLEPLRWEGYDARGVTTEAEAHKLIESWIPHIVILDWMAPSM
AGLRVLKSVRERLSHVSCVFVSENSSTEAIIEALDSGADDYIVKPFVPLELLARIRSQLRIRDLHEQLLFANEKLKELVD
TDDLTGLYNMRSLYQRLDFEMERGRRFHRDVCVVMMDMDYFKTVNDGHDHLFGSYVLSEVGKIIRANTRNIDIPARYGGD
EFLMVLTETNHAGAMYFCERLRENIEKTTFRNGEDSMKLTASLGFAITIPGENISARELVRRADHALYQAKRAGRNQVAH
YKPESAPVVEIKSAVHKRRKAAG

Sequences:

>Translated_343_residues
MSRAEVTLVCKMSFEVSPKQPKSRRILVIDDDKDSLEILLEPLRWEGYDARGVTTEAEAHKLIESWIPHIVILDWMAPSM
AGLRVLKSVRERLSHVSCVFVSENSSTEAIIEALDSGADDYIVKPFVPLELLARIRSQLRIRDLHEQLLFANEKLKELVD
TDDLTGLYNMRSLYQRLDFEMERGRRFHRDVCVVMMDMDYFKTVNDGHDHLFGSYVLSEVGKIIRANTRNIDIPARYGGD
EFLMVLTETNHAGAMYFCERLRENIEKTTFRNGEDSMKLTASLGFAITIPGENISARELVRRADHALYQAKRAGRNQVAH
YKPESAPVVEIKSAVHKRRKAAG
>Mature_342_residues
SRAEVTLVCKMSFEVSPKQPKSRRILVIDDDKDSLEILLEPLRWEGYDARGVTTEAEAHKLIESWIPHIVILDWMAPSMA
GLRVLKSVRERLSHVSCVFVSENSSTEAIIEALDSGADDYIVKPFVPLELLARIRSQLRIRDLHEQLLFANEKLKELVDT
DDLTGLYNMRSLYQRLDFEMERGRRFHRDVCVVMMDMDYFKTVNDGHDHLFGSYVLSEVGKIIRANTRNIDIPARYGGDE
FLMVLTETNHAGAMYFCERLRENIEKTTFRNGEDSMKLTASLGFAITIPGENISARELVRRADHALYQAKRAGRNQVAHY
KPESAPVVEIKSAVHKRRKAAG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=230, Percent_Identity=32.6086956521739, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693134, Length=196, Percent_Identity=36.734693877551, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787541, Length=174, Percent_Identity=32.7586206896552, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=31.7073170731707, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=231, Percent_Identity=29.8701298701299, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081881, Length=240, Percent_Identity=27.9166666666667, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082007, Length=229, Percent_Identity=27.9475982532751, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081977, Length=185, Percent_Identity=31.3513513513514, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787802, Length=238, Percent_Identity=26.0504201680672, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=28.4810126582279, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788085, Length=159, Percent_Identity=34.5911949685535, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786599, Length=128, Percent_Identity=33.59375, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=36.2068965517241, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790552, Length=116, Percent_Identity=36.2068965517241, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790863, Length=118, Percent_Identity=33.0508474576271, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788394, Length=134, Percent_Identity=29.1044776119403, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39212; Mature: 39080

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAEVTLVCKMSFEVSPKQPKSRRILVIDDDKDSLEILLEPLRWEGYDARGVTTEAEAH
CCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHH
KLIESWIPHIVILDWMAPSMAGLRVLKSVRERLSHVSCVFVSENSSTEAIIEALDSGADD
HHHHHHCCCEEEEHHHCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCC
YIVKPFVPLELLARIRSQLRIRDLHEQLLFANEKLKELVDTDDLTGLYNMRSLYQRLDFE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHH
MERGRRFHRDVCVVMMDMDYFKTVNDGHDHLFGSYVLSEVGKIIRANTRNIDIPARYGGD
HHHCHHHHHHHEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EFLMVLTETNHAGAMYFCERLRENIEKTTFRNGEDSMKLTASLGFAITIPGENISARELV
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCCHHHHH
RRADHALYQAKRAGRNQVAHYKPESAPVVEIKSAVHKRRKAAG
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRAEVTLVCKMSFEVSPKQPKSRRILVIDDDKDSLEILLEPLRWEGYDARGVTTEAEAH
CCCEEEEEEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHH
KLIESWIPHIVILDWMAPSMAGLRVLKSVRERLSHVSCVFVSENSSTEAIIEALDSGADD
HHHHHHCCCEEEEHHHCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHCCCCC
YIVKPFVPLELLARIRSQLRIRDLHEQLLFANEKLKELVDTDDLTGLYNMRSLYQRLDFE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHH
MERGRRFHRDVCVVMMDMDYFKTVNDGHDHLFGSYVLSEVGKIIRANTRNIDIPARYGGD
HHHCHHHHHHHEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EFLMVLTETNHAGAMYFCERLRENIEKTTFRNGEDSMKLTASLGFAITIPGENISARELV
EEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCCHHHHH
RRADHALYQAKRAGRNQVAHYKPESAPVVEIKSAVHKRRKAAG
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA