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The map label for this gene is phoB [H]

Identifier: 42521858

GI number: 42521858

Start: 219262

End: 220017

Strand: Direct

Name: phoB [H]

Synonym: Bd0233

Alternate gene names: 42521858

Gene position: 219262-220017 (Clockwise)

Preceding gene: 42521857

Following gene: 42521859

Centisome position: 5.8

GC content: 46.16

Gene sequence:

>756_bases
TTGCACTTTACATTTGTAGGGCTCATGCTTTTAAATATGAATATGTTTACGACTTCCACTCCTTTAAGGGGAACGGACTT
GGCTGCAAAAAAAATCGTGATTGTAGATGACTATGAAGAAAGCTGTAAGCTCCTGGCGGAGATTCTCAGCTCCACGTATG
AGTGTTCTTACACTTCTGACAGCACTTCTGCGTTCCGCTTGATCAATGAAAAACGCCCGGATCTGATTCTGCTGGATTAT
AAAATGCCAGGGGTGATGGGTGTGGATGTGTGTCGTATGGTTCGCGAAAGTGATTCCACGAAAAACACGCCGATCATCTT
CGTTTCCGGTGCGGCGACGATTGATGAAAGAATCAAAGCGTTTGAAACCGGTGCTGATGACTTCATCTCAAAGCCTTTCC
ACGTGAAAGAGCTGATTCTAAGAATCAAAGCCCGTCTTTCAGAAAAAGAGCCGGAAGCGGCATCTGAGCTGAATGCGGCG
AATATGAAGATGAACCTTCTTTCCCGCCAGGTGTTTGTGGATGGTGAAGAAGTGAACCTGACGCCAAAACAGTTCGATAT
TCTGAAGCTTCTGGTGGCCGGAAAGAACAATCTGGTCACTCGGGAAAAATGTCTTTCAGAAATCTGGGGCGATACGGAAG
TGACGTCCCGAAATGTGGATTCACAGATCAATTATCTGAAACGTAAAATCCATAAATTCAACGGCCGCATCGTTGCGGTT
CCGTCCTTGGGCTATCGCCTGGAAGCTCAGGAATAG

Upstream 100 bases:

>100_bases
GATGATCGAAATCCAGGGTGACAAAAGAGACCAATTGAAGAAGATTCTGGAAGCGAAGAAGATCAAATTTAAAGGCATGT
AAAACTGGACCCTGTGCAAC

Downstream 100 bases:

>100_bases
TTCCCCAAAATATCACAAAGAGGCTTGCCCCAGGGGTTTTATGGGTCCACACTTAATGTATGGACGCAGCAAAACCCTTG
CTGATTGGTCTTTCTCCCCG

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MHFTFVGLMLLNMNMFTTSTPLRGTDLAAKKIVIVDDYEESCKLLAEILSSTYECSYTSDSTSAFRLINEKRPDLILLDY
KMPGVMGVDVCRMVRESDSTKNTPIIFVSGAATIDERIKAFETGADDFISKPFHVKELILRIKARLSEKEPEAASELNAA
NMKMNLLSRQVFVDGEEVNLTPKQFDILKLLVAGKNNLVTREKCLSEIWGDTEVTSRNVDSQINYLKRKIHKFNGRIVAV
PSLGYRLEAQE

Sequences:

>Translated_251_residues
MHFTFVGLMLLNMNMFTTSTPLRGTDLAAKKIVIVDDYEESCKLLAEILSSTYECSYTSDSTSAFRLINEKRPDLILLDY
KMPGVMGVDVCRMVRESDSTKNTPIIFVSGAATIDERIKAFETGADDFISKPFHVKELILRIKARLSEKEPEAASELNAA
NMKMNLLSRQVFVDGEEVNLTPKQFDILKLLVAGKNNLVTREKCLSEIWGDTEVTSRNVDSQINYLKRKIHKFNGRIVAV
PSLGYRLEAQE
>Mature_251_residues
MHFTFVGLMLLNMNMFTTSTPLRGTDLAAKKIVIVDDYEESCKLLAEILSSTYECSYTSDSTSAFRLINEKRPDLILLDY
KMPGVMGVDVCRMVRESDSTKNTPIIFVSGAATIDERIKAFETGADDFISKPFHVKELILRIKARLSEKEPEAASELNAA
NMKMNLLSRQVFVDGEEVNLTPKQFDILKLLVAGKNNLVTREKCLSEIWGDTEVTSRNVDSQINYLKRKIHKFNGRIVAV
PSLGYRLEAQE

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=29.4642857142857, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=31.5789473684211, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790552, Length=226, Percent_Identity=31.4159292035398, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786784, Length=232, Percent_Identity=27.5862068965517, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=31.2775330396476, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=28.2511210762332, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789402, Length=177, Percent_Identity=31.0734463276836, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1786911, Length=183, Percent_Identity=27.8688524590164, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=22.7272727272727, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI2367329, Length=210, Percent_Identity=30.952380952381, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790860, Length=183, Percent_Identity=24.5901639344262, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787229, Length=184, Percent_Identity=27.7173913043478, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=23.6286919831224, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788550, Length=121, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28311; Mature: 28311

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFTFVGLMLLNMNMFTTSTPLRGTDLAAKKIVIVDDYEESCKLLAEILSSTYECSYTSD
CCHHHEEHHHHHCHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCC
STSAFRLINEKRPDLILLDYKMPGVMGVDVCRMVRESDSTKNTPIIFVSGAATIDERIKA
CHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHH
FETGADDFISKPFHVKELILRIKARLSEKEPEAASELNAANMKMNLLSRQVFVDGEEVNL
HHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCC
TPKQFDILKLLVAGKNNLVTREKCLSEIWGDTEVTSRNVDSQINYLKRKIHKFNGRIVAV
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
PSLGYRLEAQE
CCCCEEEECCC
>Mature Secondary Structure
MHFTFVGLMLLNMNMFTTSTPLRGTDLAAKKIVIVDDYEESCKLLAEILSSTYECSYTSD
CCHHHEEHHHHHCHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEECCCCC
STSAFRLINEKRPDLILLDYKMPGVMGVDVCRMVRESDSTKNTPIIFVSGAATIDERIKA
CHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHH
FETGADDFISKPFHVKELILRIKARLSEKEPEAASELNAANMKMNLLSRQVFVDGEEVNL
HHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCC
TPKQFDILKLLVAGKNNLVTREKCLSEIWGDTEVTSRNVDSQINYLKRKIHKFNGRIVAV
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
PSLGYRLEAQE
CCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]