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The map label for this gene is queF

Identifier: 42521732

GI number: 42521732

Start: 83103

End: 83615

Strand: Direct

Name: queF

Synonym: Bd0087

Alternate gene names: 42521732

Gene position: 83103-83615 (Clockwise)

Preceding gene: 42521731

Following gene: 42521733

Centisome position: 2.2

GC content: 46.98

Gene sequence:

>513_bases
ATGAAAAAGAAAAACGGCCGTGATTCCAAAGAGCTTGCTGATTTCGCCTTGGGTGAAAGTCAGACCAACTATCCAGAAAC
ATATGCTCCAGAAGTTCTGGAGGCCTTTGACAACAAAAACCCGGGCAAGATCGCCTGGACCACTTTTGTATGTACTGAAT
TCACGTCCCTGTGCCCTAAGACCCGTCAGCCGGATTTCGCAAAGATCTTCATCAACTACATTGCTGACAAAAAAATGGTC
GAATCCAAATCCCTGAAACTGTATCTGTTCAGCTTCCGTAACCACGGGGACTTCCACGAAGACTGCGTGCAGACCATCTG
TGATGATCTGGTGAAGTTGATGAAGCCTAAATACATCGAAGTTATCGGCGAGTTCACACCGCGCGGGGGGATTGCGATTT
ACCCTTACGCGAACTATGCGGCGAAAGATAAGTTCTTCCAGGAACTTTATAAAAAGCGCATGAGCGAATACGCTCCGGGC
AAGTATTCTATGGAGCTATCCAAGCTCTATTAG

Upstream 100 bases:

>100_bases
GGCTATTGGCTGGCTCGTTACATTGGCGCCACCAATATCAACTAGGCGCCTGTAAAAGATACCGTTGCTAATCATGGGGA
TATCTCTTAAAAAGGTCCCC

Downstream 100 bases:

>100_bases
AGCTGGTACCTCTTGAGAGCGCTTTGCTCTCAAGAGCCCACAAATTGAACTGATTTCACAAAAACGTCGAATTGATGGTC
GTTCAAGTCTCGGGCTTCGC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MKKKNGRDSKELADFALGESQTNYPETYAPEVLEAFDNKNPGKIAWTTFVCTEFTSLCPKTRQPDFAKIFINYIADKKMV
ESKSLKLYLFSFRNHGDFHEDCVQTICDDLVKLMKPKYIEVIGEFTPRGGIAIYPYANYAAKDKFFQELYKKRMSEYAPG
KYSMELSKLY

Sequences:

>Translated_170_residues
MKKKNGRDSKELADFALGESQTNYPETYAPEVLEAFDNKNPGKIAWTTFVCTEFTSLCPKTRQPDFAKIFINYIADKKMV
ESKSLKLYLFSFRNHGDFHEDCVQTICDDLVKLMKPKYIEVIGEFTPRGGIAIYPYANYAAKDKFFQELYKKRMSEYAPG
KYSMELSKLY
>Mature_170_residues
MKKKNGRDSKELADFALGESQTNYPETYAPEVLEAFDNKNPGKIAWTTFVCTEFTSLCPKTRQPDFAKIFINYIADKKMV
ESKSLKLYLFSFRNHGDFHEDCVQTICDDLVKLMKPKYIEVIGEFTPRGGIAIYPYANYAAKDKFFQELYKKRMSEYAPG
KYSMELSKLY

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=94, Percent_Identity=36.1702127659575, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_BDEBA (Q6MRJ2)

Other databases:

- EMBL:   BX842646
- RefSeq:   NP_967112.1
- ProteinModelPortal:   Q6MRJ2
- SMR:   Q6MRJ2
- GeneID:   2734018
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0087
- NMPDR:   fig|264462.1.peg.82
- HOGENOM:   HBG294103
- OMA:   FATLYIN
- BioCyc:   BBAC264462:BD0087-MONOMER
- BRENDA:   1.7.1.13
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 19733; Mature: 19733

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKNGRDSKELADFALGESQTNYPETYAPEVLEAFDNKNPGKIAWTTFVCTEFTSLCPK
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCC
TRQPDFAKIFINYIADKKMVESKSLKLYLFSFRNHGDFHEDCVQTICDDLVKLMKPKYIE
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHH
VIGEFTPRGGIAIYPYANYAAKDKFFQELYKKRMSEYAPGKYSMELSKLY
HHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC
>Mature Secondary Structure
MKKKNGRDSKELADFALGESQTNYPETYAPEVLEAFDNKNPGKIAWTTFVCTEFTSLCPK
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHCCC
TRQPDFAKIFINYIADKKMVESKSLKLYLFSFRNHGDFHEDCVQTICDDLVKLMKPKYIE
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHH
VIGEFTPRGGIAIYPYANYAAKDKFFQELYKKRMSEYAPGKYSMELSKLY
HHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA