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The map label for this gene is gatB

Identifier: 42521707

GI number: 42521707

Start: 54440

End: 55873

Strand: Direct

Name: gatB

Synonym: Bd0060

Alternate gene names: 42521707

Gene position: 54440-55873 (Clockwise)

Preceding gene: 42521706

Following gene: 42521708

Centisome position: 1.44

GC content: 50.21

Gene sequence:

>1434_bases
ATGTCATCTAGAGGATATGAAGCCGTCATCGGTATTGAGATCCACGTGCAGCTGAGCACAAAGACCAAAATCTTCAGCTC
CGAATCCACGGCCTTTGAAGCGGGTGACAATGAAAACACCTCGCCGGTCAGTGTGGGCATGCCCGGCACCCTGCCGGTCT
TAAACAGCAAGGTGGTTGAGTACTCGATTAAAACGGGGCTTGCTTTGGGTTGTGACATCCGCCGCAAATCTGTTTTCGCC
CGCAAGAACTATTTCTATCCGGATCTTCCCAAGGGTTATCAGATCTCCCAGTATGATCAGCCTATTTGTGAAAACGGATC
CATCACATTCAAAGTGGATGGAAAAGAAAAGACCGTATCCATCACCCGCGCCCATATGGAAGAAGATGCCGGCAAATCCA
ACCACCACGGTGAATACACACTGATCAACTACAACCGTTCCGGCATTCCATTATTAGAGGTCGTATCGGGCCCCGACATG
CGCACGCCGCAGGAGGCAGCAGAATACGCACGCACCATTCGTCAGATTGTGCGCTATTTGGATGTCTGTGACGGAAATCT
GGAAGAGGGATCGTTGCGCTGTGACTGCAATGTCTCGGTTCGCAAAGAGGGTGCTAAACAATTCGGAACCAAAGTTGAGA
TCAAAAACATCAATTCGTTCCGTTTTGTCGAAAAAGCCATCGAATATGAAATTGAACGTCAGATCGACTGTGTTGAGCGC
GGTGACAAGATCATCCAGGAAACCCGTTTGTGGGATCCGGATAAAAACCGCACGTTCTCGATGCGCGCCAAAGAAGACGC
GCAGGACTATCGCTATTTCCCGGATCCGGATCTGCAGCCGGTGATTGTCACAGACAGCATGATTGAAAAGTATAAAAAAG
AACTGCCCGAGCTTCCGATTGCCCGCGCCCAGCGCTTCCAGGATGATCACGCGTTGCCGGAATTGGATGCCACGGTGCTG
ACCACGGAAAAAGATCTGGCCGACTTCTATGAAGACACCGCAAAAGAATCCAAAAACTTCAAGGCGTCCTCGAACTGGAT
CATGACCGAGCTTTTGCGCGAGCTGAATTCCGCCAATAAAAACATCAAGGATTCGCCGATCAAACCGGCGCAGCTGGGCA
AAATGATTGCGATGATCGACAAGGGAACAATTTCCGGTAAGATTGCAAAAACCATCTTCCAAGAGATGTGGGCCTCTGGA
AATGATCCGGAAGTCATCATGAAGGAAAAGGGTCTGGTGCAGATCTCGGATCCCGCGGCGATTGAAAAGCTGGTGGATGA
AGTATTGGCGGCCAACGCGCAGACCGTGGAAGATCACAAGTCCGGCAAAAAAAAGAACCTCTTTGGTTTCTTTGTCGGTG
CCGTGATGAAGGCCTCCAAGGGGCAGGCCAATCCGGACCTTGTGAACAAGATTCTTCTAGAGAAACTGAAATAG

Upstream 100 bases:

>100_bases
ATCGGGATTCAGCTGACCGCCGGTCACTTTGAAGAACAAAAGATGCTGAACGTGGCCTTTGCGCTGGAAGGTGCCTCTTT
GGTGAAAGGAAAACATCCCC

Downstream 100 bases:

>100_bases
AACAGGATATTTGAAATGAAAAAAGTTGCAGCACTTGATCTTGGAACCAACACCTTCCTGTGCCTGATTGTTTCTGGTGA
CAAAGATGGAATCAAAAAGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSSRGYEAVIGIEIHVQLSTKTKIFSSESTAFEAGDNENTSPVSVGMPGTLPVLNSKVVEYSIKTGLALGCDIRRKSVFA
RKNYFYPDLPKGYQISQYDQPICENGSITFKVDGKEKTVSITRAHMEEDAGKSNHHGEYTLINYNRSGIPLLEVVSGPDM
RTPQEAAEYARTIRQIVRYLDVCDGNLEEGSLRCDCNVSVRKEGAKQFGTKVEIKNINSFRFVEKAIEYEIERQIDCVER
GDKIIQETRLWDPDKNRTFSMRAKEDAQDYRYFPDPDLQPVIVTDSMIEKYKKELPELPIARAQRFQDDHALPELDATVL
TTEKDLADFYEDTAKESKNFKASSNWIMTELLRELNSANKNIKDSPIKPAQLGKMIAMIDKGTISGKIAKTIFQEMWASG
NDPEVIMKEKGLVQISDPAAIEKLVDEVLAANAQTVEDHKSGKKKNLFGFFVGAVMKASKGQANPDLVNKILLEKLK

Sequences:

>Translated_477_residues
MSSRGYEAVIGIEIHVQLSTKTKIFSSESTAFEAGDNENTSPVSVGMPGTLPVLNSKVVEYSIKTGLALGCDIRRKSVFA
RKNYFYPDLPKGYQISQYDQPICENGSITFKVDGKEKTVSITRAHMEEDAGKSNHHGEYTLINYNRSGIPLLEVVSGPDM
RTPQEAAEYARTIRQIVRYLDVCDGNLEEGSLRCDCNVSVRKEGAKQFGTKVEIKNINSFRFVEKAIEYEIERQIDCVER
GDKIIQETRLWDPDKNRTFSMRAKEDAQDYRYFPDPDLQPVIVTDSMIEKYKKELPELPIARAQRFQDDHALPELDATVL
TTEKDLADFYEDTAKESKNFKASSNWIMTELLRELNSANKNIKDSPIKPAQLGKMIAMIDKGTISGKIAKTIFQEMWASG
NDPEVIMKEKGLVQISDPAAIEKLVDEVLAANAQTVEDHKSGKKKNLFGFFVGAVMKASKGQANPDLVNKILLEKLK
>Mature_476_residues
SSRGYEAVIGIEIHVQLSTKTKIFSSESTAFEAGDNENTSPVSVGMPGTLPVLNSKVVEYSIKTGLALGCDIRRKSVFAR
KNYFYPDLPKGYQISQYDQPICENGSITFKVDGKEKTVSITRAHMEEDAGKSNHHGEYTLINYNRSGIPLLEVVSGPDMR
TPQEAAEYARTIRQIVRYLDVCDGNLEEGSLRCDCNVSVRKEGAKQFGTKVEIKNINSFRFVEKAIEYEIERQIDCVERG
DKIIQETRLWDPDKNRTFSMRAKEDAQDYRYFPDPDLQPVIVTDSMIEKYKKELPELPIARAQRFQDDHALPELDATVLT
TEKDLADFYEDTAKESKNFKASSNWIMTELLRELNSANKNIKDSPIKPAQLGKMIAMIDKGTISGKIAKTIFQEMWASGN
DPEVIMKEKGLVQISDPAAIEKLVDEVLAANAQTVEDHKSGKKKNLFGFFVGAVMKASKGQANPDLVNKILLEKLK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=39.3145161290323, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI115534094, Length=465, Percent_Identity=30.752688172043, Blast_Score=207, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=508, Percent_Identity=31.1023622047244, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=37.0221327967807, Blast_Score=311, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_BDEBA (P61341)

Other databases:

- EMBL:   BX842646
- RefSeq:   NP_967087.1
- ProteinModelPortal:   P61341
- SMR:   P61341
- GeneID:   2733049
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0060
- NMPDR:   fig|264462.1.peg.57
- HOGENOM:   HBG395951
- OMA:   RIHGATH
- PhylomeDB:   P61341
- ProtClustDB:   PRK05477
- BioCyc:   BBAC264462:BD0060-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53590; Mature: 53458

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRGYEAVIGIEIHVQLSTKTKIFSSESTAFEAGDNENTSPVSVGMPGTLPVLNSKVVE
CCCCCCEEEEEEEEEEEEECCEEEECCCCCEEECCCCCCCCCEEECCCCCCHHHCCHHHE
YSIKTGLALGCDIRRKSVFARKNYFYPDLPKGYQISQYDQPICENGSITFKVDGKEKTVS
EEHHCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEE
ITRAHMEEDAGKSNHHGEYTLINYNRSGIPLLEVVSGPDMRTPQEAAEYARTIRQIVRYL
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DVCDGNLEEGSLRCDCNVSVRKEGAKQFGTKVEIKNINSFRFVEKAIEYEIERQIDCVER
HHCCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
GDKIIQETRLWDPDKNRTFSMRAKEDAQDYRYFPDPDLQPVIVTDSMIEKYKKELPELPI
HHHHHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCH
ARAQRFQDDHALPELDATVLTTEKDLADFYEDTAKESKNFKASSNWIMTELLRELNSANK
HHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
NIKDSPIKPAQLGKMIAMIDKGTISGKIAKTIFQEMWASGNDPEVIMKEKGLVQISDPAA
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCHHH
IEKLVDEVLAANAQTVEDHKSGKKKNLFGFFVGAVMKASKGQANPDLVNKILLEKLK
HHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SSRGYEAVIGIEIHVQLSTKTKIFSSESTAFEAGDNENTSPVSVGMPGTLPVLNSKVVE
CCCCCEEEEEEEEEEEEECCEEEECCCCCEEECCCCCCCCCEEECCCCCCHHHCCHHHE
YSIKTGLALGCDIRRKSVFARKNYFYPDLPKGYQISQYDQPICENGSITFKVDGKEKTVS
EEHHCCCEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEE
ITRAHMEEDAGKSNHHGEYTLINYNRSGIPLLEVVSGPDMRTPQEAAEYARTIRQIVRYL
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
DVCDGNLEEGSLRCDCNVSVRKEGAKQFGTKVEIKNINSFRFVEKAIEYEIERQIDCVER
HHCCCCCCCCCEEEECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
GDKIIQETRLWDPDKNRTFSMRAKEDAQDYRYFPDPDLQPVIVTDSMIEKYKKELPELPI
HHHHHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCH
ARAQRFQDDHALPELDATVLTTEKDLADFYEDTAKESKNFKASSNWIMTELLRELNSANK
HHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
NIKDSPIKPAQLGKMIAMIDKGTISGKIAKTIFQEMWASGNDPEVIMKEKGLVQISDPAA
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCHHH
IEKLVDEVLAANAQTVEDHKSGKKKNLFGFFVGAVMKASKGQANPDLVNKILLEKLK
HHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA