Click here to switch to the map view.

The map label for this gene is napA

Identifier: 38637875

GI number: 38637875

Start: 228154

End: 230649

Strand: Direct

Name: napA

Synonym: PHG211

Alternate gene names: 38637875

Gene position: 228154-230649 (Clockwise)

Preceding gene: 38637874

Following gene: 38637876

Centisome position: 50.46

GC content: 64.26

Gene sequence:

>2496_bases
ATGAAGATCTCTCGTCGTGATTTCATCAAGCAGACCGCGATCACGGCTACCGCATCCGTGGCAGGCGTGACCCTGCCAGC
GGGGGCCGCCAACTTTGTCACAGACAGCGAGGTGACCAAGCTGAAGTGGTCAAAGGCGCCATGCCGGTTCTGCGGCACAG
GCTGCGGCGTCACCGTCGCCGTCAAGGACAACAAGGTGGTGGCCACGCAAGGCGATCCGCAGGCCGAGGTCAACAAGGGC
CTGAACTGCGTCAAGGGCTACTTCCTGTCCAAGATCATGTACGGGCAGGACCGGCTCACCAGGCCGCTCATGCGCATGAA
GAACGGCAAGTACGACAAGAACGGCGATTTCGCGCCGGTCACCTGGGACCAGGCGTTCGACGAGATGGAGCGCCAGTTCA
AGCGCGTGCTGAAGGAAAAGGGTCCGACCGCGGTCGGCATGTTCGGCTCCGGCCAGTGGACGGTATGGGAAGGCTACGCG
GCCGCCAAGCTCTACAAGGCAGGCTTCCGCTCCAACAACATCGACCCCAATGCGCGGCATTGCATGGCATCGGCCGCGGC
CGGCTTCATGCGCACCTTCGGCATGGACGAGCCGATGGGCTGCTATGACGACTTCGAGGCCGCCGATGCCTTCGTGCTGT
GGGGCTCGAACATGGCAGAGATGCACCCGATCCTGTGGACCCGCGTCACCGACCGCCGGCTGAGCCACCCCAAGACCCGC
GTGGTGGTGCTCTCCACCTTCACGCACCGGTGCTTCGACCTGGCCGACATTGGCATCATCTTCAAGCCGCAGACTGACCT
GGCGATGCTCAACTACATCGCCAACTACATCATCCGCAACAACAAGGTCAACAAGGACTTCGTCAACAAGCACACCGTCT
TCAAAGAGGGCGTGACCGACATCGGCTACGGCCTGCGCCCGGACCATCCGCTGCAGAAGGCGGCCAAGAATGCCTCCGAC
CCCGGCGCGGCCAAAGTCATCACCTTCGACGAATTCGCGAAATTCGTCTCGAAGTATGACGCCGACTACGTCAGCAAGCT
GTCGGCCGTGCCCAAGGCCAAGCTCGACCAGCTCGCCGAACTGTACGCCGACCCCAACATCAAGGTCATGTCGCTGTGGA
CCATGGGCTTCAACCAGCACACGCGCGGAACGTGGGCCAACAACATGGTCTACAACCTGCACTTGCTGACCGGCAAGATC
GCCACGCCGGGCAACAGCCCGTTCTCACTGACGGGGCAACCCTCCGCCTGCGGCACCGCGCGCGAGGTCGGCACCTTCTC
GCACCGGCTGCCGGCGGACATGGTGGTCACCAACCCAAAGCATCGCGAAGAGGCCGAGCGCATCTGGAAGCTGCCGCCTG
GCACCATCCCCGACAAGCCCGGCTACGACGCCGTGCTGCAGAACCGCATGCTCAAGGACGGCAAGCTCAACGCCTACTGG
GTGCAGGTCAATAACAACATGCAGGCCGCCGCCAACCTGATGGAGGAAGGGCTGCCGGGCTACCGGAATCCCGCCAACTT
CATCGTAGTGTCGGACGCCTACCCCACCGTGACCGCGCTGGCCGCCGACCTGGTCCTGCCCAGCGCGATGTGGGTCGAGA
AGGAAGGCGCCTACGGCAACGCCGAACGCCGCACGCAGTTCTGGCACCAGCTGGTCGATGCTCCGGGCGAGGCGCGCTCG
GACCTGTGGCAGCTGGTGGAGTTCGCCAAGCGCTTCAAGGTGGAAGAGGTCTGGCCGCCCGAGCTGATCGCCAAAAAGCC
GGAGTACAAGGGCAAGACCTTGTATGACGTGCTCTACCGCAACGGCCAGGTCGACAAGTTCCCGCTCAAGGACGTCAACG
CCGAATACCACAATGCCGAGGCCAAGGCCTTCGGCTTCTATCTCCAGAAGGGCCTGTTCGAGGAATACGCCACCTTCGGC
CGCGGTCACGGCCATGACCTGGCGCCGTTCGACGCCTACCACGAGGCGCGCGGCCTGCGCTGGCCCGTGGTCAACGGCAA
GGAGACCCGCTGGCGCTACCGCGAAGGCAGCGACCCATACGTCAAGGCCGGCACCGGCTTCCAGTTCTACGGCAACCCCG
ACGGCAAGGCGGTGATCTTCGCCCTGCCCTACGAGCCGCCGGCCGAATCGCCCGACAAGGAATACCCGTACTGGCTAGTC
ACCGGCCGCGTGCTGGAGCACTGGCACTCCGGATCGATGACGCGGCGCGTGCCGGAGCTTTACCGTTCCTTCCCCAATGC
GGTGGTGTTCATGCACCCGGAAGACGCGAAAGCGTTGGGGCTGCGCCGTGGCGTGGAGGTCGAGGTGGTGTCGCGGCGCG
GCCGCATGCGCTCCCGCATCGAGACACGCGGGCGCGACGCGCCGCCGCGCGGGCTGGTGTTCGTGCCGTGGTTCGACGCC
AGCCAGCTGATCAACAAGGTAACGCTGGACGCTACCTGCCCGATCTCGCTGCAGACCGACTTCAAGAAGTGCGCGGTCAA
GATCGTGAAGGTATAG

Upstream 100 bases:

>100_bases
GCACACCTCACCTGCCTCCACCAGTTGGAGGGCGTGCTCTCCGCCGCGCTGGTCTACCAGCACAACGAAGACGCCGCAGC
GATGAACGAGGAGATGGCCG

Downstream 100 bases:

>100_bases
CGTCGGTCGAGACCAAGAACAGGGAGACGCCATGAAGCCAAGCCGATCCTGGGCAAGCCTGCTGGCCGTCTGCGCGGTGC
TGCTGGCCGCGCTGGCCATG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MKISRRDFIKQTAITATASVAGVTLPAGAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVKDNKVVATQGDPQAEVNKG
LNCVKGYFLSKIMYGQDRLTRPLMRMKNGKYDKNGDFAPVTWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYA
AAKLYKAGFRSNNIDPNARHCMASAAAGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRVTDRRLSHPKTR
VVVLSTFTHRCFDLADIGIIFKPQTDLAMLNYIANYIIRNNKVNKDFVNKHTVFKEGVTDIGYGLRPDHPLQKAAKNASD
PGAAKVITFDEFAKFVSKYDADYVSKLSAVPKAKLDQLAELYADPNIKVMSLWTMGFNQHTRGTWANNMVYNLHLLTGKI
ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYDAVLQNRMLKDGKLNAYW
VQVNNNMQAAANLMEEGLPGYRNPANFIVVSDAYPTVTALAADLVLPSAMWVEKEGAYGNAERRTQFWHQLVDAPGEARS
DLWQLVEFAKRFKVEEVWPPELIAKKPEYKGKTLYDVLYRNGQVDKFPLKDVNAEYHNAEAKAFGFYLQKGLFEEYATFG
RGHGHDLAPFDAYHEARGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPAESPDKEYPYWLV
TGRVLEHWHSGSMTRRVPELYRSFPNAVVFMHPEDAKALGLRRGVEVEVVSRRGRMRSRIETRGRDAPPRGLVFVPWFDA
SQLINKVTLDATCPISLQTDFKKCAVKIVKV

Sequences:

>Translated_831_residues
MKISRRDFIKQTAITATASVAGVTLPAGAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVKDNKVVATQGDPQAEVNKG
LNCVKGYFLSKIMYGQDRLTRPLMRMKNGKYDKNGDFAPVTWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYA
AAKLYKAGFRSNNIDPNARHCMASAAAGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRVTDRRLSHPKTR
VVVLSTFTHRCFDLADIGIIFKPQTDLAMLNYIANYIIRNNKVNKDFVNKHTVFKEGVTDIGYGLRPDHPLQKAAKNASD
PGAAKVITFDEFAKFVSKYDADYVSKLSAVPKAKLDQLAELYADPNIKVMSLWTMGFNQHTRGTWANNMVYNLHLLTGKI
ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYDAVLQNRMLKDGKLNAYW
VQVNNNMQAAANLMEEGLPGYRNPANFIVVSDAYPTVTALAADLVLPSAMWVEKEGAYGNAERRTQFWHQLVDAPGEARS
DLWQLVEFAKRFKVEEVWPPELIAKKPEYKGKTLYDVLYRNGQVDKFPLKDVNAEYHNAEAKAFGFYLQKGLFEEYATFG
RGHGHDLAPFDAYHEARGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPAESPDKEYPYWLV
TGRVLEHWHSGSMTRRVPELYRSFPNAVVFMHPEDAKALGLRRGVEVEVVSRRGRMRSRIETRGRDAPPRGLVFVPWFDA
SQLINKVTLDATCPISLQTDFKKCAVKIVKV
>Mature_831_residues
MKISRRDFIKQTAITATASVAGVTLPAGAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVAVKDNKVVATQGDPQAEVNKG
LNCVKGYFLSKIMYGQDRLTRPLMRMKNGKYDKNGDFAPVTWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYA
AAKLYKAGFRSNNIDPNARHCMASAAAGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRVTDRRLSHPKTR
VVVLSTFTHRCFDLADIGIIFKPQTDLAMLNYIANYIIRNNKVNKDFVNKHTVFKEGVTDIGYGLRPDHPLQKAAKNASD
PGAAKVITFDEFAKFVSKYDADYVSKLSAVPKAKLDQLAELYADPNIKVMSLWTMGFNQHTRGTWANNMVYNLHLLTGKI
ATPGNSPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYDAVLQNRMLKDGKLNAYW
VQVNNNMQAAANLMEEGLPGYRNPANFIVVSDAYPTVTALAADLVLPSAMWVEKEGAYGNAERRTQFWHQLVDAPGEARS
DLWQLVEFAKRFKVEEVWPPELIAKKPEYKGKTLYDVLYRNGQVDKFPLKDVNAEYHNAEAKAFGFYLQKGLFEEYATFG
RGHGHDLAPFDAYHEARGLRWPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPAESPDKEYPYWLV
TGRVLEHWHSGSMTRRVPELYRSFPNAVVFMHPEDAKALGLRRGVEVEVVSRRGRMRSRIETRGRDAPPRGLVFVPWFDA
SQLINKVTLDATCPISLQTDFKKCAVKIVKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=833, Percent_Identity=69.7478991596639, Blast_Score=1248, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=794, Percent_Identity=24.1813602015113, Blast_Score=206, Evalue=5e-54,
Organism=Escherichia coli, GI3868720, Length=664, Percent_Identity=23.4939759036145, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI3868719, Length=660, Percent_Identity=22.7272727272727, Blast_Score=118, Evalue=2e-27,
Organism=Escherichia coli, GI87081797, Length=851, Percent_Identity=21.8566392479436, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1787870, Length=626, Percent_Identity=24.6006389776358, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI171474008, Length=817, Percent_Identity=22.6438188494492, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_CUPNH (P39185)

Other databases:

- EMBL:   X71385
- EMBL:   AY305378
- PIR:   A48489
- RefSeq:   NP_942849.1
- ProteinModelPortal:   P39185
- SMR:   P39185
- GeneID:   2656653
- GenomeReviews:   AY305378_GR
- KEGG:   reh:PHG211
- eggNOG:   COG0243
- HOGENOM:   HBG715033
- OMA:   QFWHQLV
- PhylomeDB:   P39185
- ProtClustDB:   PRK13532
- BioCyc:   REUT381666:PHG211-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 93347; Mature: 93347

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISRRDFIKQTAITATASVAGVTLPAGAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVA
CCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHEEECCCCCHHHCCCCCCEEEE
VKDNKVVATQGDPQAEVNKGLNCVKGYFLSKIMYGQDRLTRPLMRMKNGKYDKNGDFAPV
EECCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCC
TWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYAAAKLYKAGFRSNNIDPNARH
CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAAAGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRVTDRRLSHPKTR
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCCHHHHCCHHHEEEHHHHHCCCCEE
VVVLSTFTHRCFDLADIGIIFKPQTDLAMLNYIANYIIRNNKVNKDFVNKHTVFKEGVTD
EEEEECHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHH
IGYGLRPDHPLQKAAKNASDPGAAKVITFDEFAKFVSKYDADYVSKLSAVPKAKLDQLAE
CCCCCCCCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHH
LYADPNIKVMSLWTMGFNQHTRGTWANNMVYNLHLLTGKIATPGNSPFSLTGQPSACGTA
HHCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCH
REVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYDAVLQNRMLKDGKLNAYW
HHHCCHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEE
VQVNNNMQAAANLMEEGLPGYRNPANFIVVSDAYPTVTALAADLVLPSAMWVEKEGAYGN
EEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHEEECCCCCCC
AERRTQFWHQLVDAPGEARSDLWQLVEFAKRFKVEEVWPPELIAKKPEYKGKTLYDVLYR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHC
NGQVDKFPLKDVNAEYHNAEAKAFGFYLQKGLFEEYATFGRGHGHDLAPFDAYHEARGLR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
WPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPAESPDKEYPYWLV
CCCCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEE
TGRVLEHWHSGSMTRRVPELYRSFPNAVVFMHPEDAKALGLRRGVEVEVVSRRGRMRSRI
EHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCEEEEHHHHHHHHHHH
ETRGRDAPPRGLVFVPWFDASQLINKVTLDATCPISLQTDFKKCAVKIVKV
HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCEECCCCHHHHHHEEECC
>Mature Secondary Structure
MKISRRDFIKQTAITATASVAGVTLPAGAANFVTDSEVTKLKWSKAPCRFCGTGCGVTVA
CCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHEEECCCCCHHHCCCCCCEEEE
VKDNKVVATQGDPQAEVNKGLNCVKGYFLSKIMYGQDRLTRPLMRMKNGKYDKNGDFAPV
EECCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCC
TWDQAFDEMERQFKRVLKEKGPTAVGMFGSGQWTVWEGYAAAKLYKAGFRSNNIDPNARH
CHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAAAGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMAEMHPILWTRVTDRRLSHPKTR
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCCHHHHCCHHHEEEHHHHHCCCCEE
VVVLSTFTHRCFDLADIGIIFKPQTDLAMLNYIANYIIRNNKVNKDFVNKHTVFKEGVTD
EEEEECHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHH
IGYGLRPDHPLQKAAKNASDPGAAKVITFDEFAKFVSKYDADYVSKLSAVPKAKLDQLAE
CCCCCCCCCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHH
LYADPNIKVMSLWTMGFNQHTRGTWANNMVYNLHLLTGKIATPGNSPFSLTGQPSACGTA
HHCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEECCCCCCCCCH
REVGTFSHRLPADMVVTNPKHREEAERIWKLPPGTIPDKPGYDAVLQNRMLKDGKLNAYW
HHHCCHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEE
VQVNNNMQAAANLMEEGLPGYRNPANFIVVSDAYPTVTALAADLVLPSAMWVEKEGAYGN
EEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCHHEEECCCCCCC
AERRTQFWHQLVDAPGEARSDLWQLVEFAKRFKVEEVWPPELIAKKPEYKGKTLYDVLYR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHC
NGQVDKFPLKDVNAEYHNAEAKAFGFYLQKGLFEEYATFGRGHGHDLAPFDAYHEARGLR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
WPVVNGKETRWRYREGSDPYVKAGTGFQFYGNPDGKAVIFALPYEPPAESPDKEYPYWLV
CCCCCCCCCCEEECCCCCCEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEE
TGRVLEHWHSGSMTRRVPELYRSFPNAVVFMHPEDAKALGLRRGVEVEVVSRRGRMRSRI
EHHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCEEEEHHHHHHHHHHH
ETRGRDAPPRGLVFVPWFDASQLINKVTLDATCPISLQTDFKKCAVKIVKV
HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCEECCCCHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488