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The map label for this gene is napB

Identifier: 38637876

GI number: 38637876

Start: 230681

End: 231190

Strand: Direct

Name: napB

Synonym: PHG212

Alternate gene names: 38637876

Gene position: 230681-231190 (Clockwise)

Preceding gene: 38637875

Following gene: 38637877

Centisome position: 51.02

GC content: 65.88

Gene sequence:

>510_bases
ATGAAGCCAAGCCGATCCTGGGCAAGCCTGCTGGCCGTCTGCGCGGTGCTGCTGGCCGCGCTGGCCATGCAGGCGATCTT
TTTCCCCGCGCCCGCGCGCGCGCAGGGCCTGGTGGATGCCATGCGCGGCCCCACCGCCATCGCCAACGAGCCGCGCGCCC
CGCTGCTCTACCCCACAGAGAACAAGGACATCCGCCGCACGCGCAACTACACCATGCAGCCGCCGACGATCCCGCACAAG
ATCGACGGCTACCAGCTGGACAAGGACTTCAACCGCTGCATGTTCTGCCACGCGCGCACCCGCACCGAAGAGACCCAGGC
GATCCCGGTCAGCATCACGCACTACATGGATCGCGACAACAACGTGCTGGCCGATGTTTCGCCGCGGCGCTATTTCTGCA
CCCAATGCCACGTACCGCAGGCCGATACTAAGCCGCTGATCGGCAACAACTTCGTCGACGTGGACACCATCCTCAAGCGC
AGGCCCGGCGCCAAGGGCGCCGCCAAATAA

Upstream 100 bases:

>100_bases
GACGCTACCTGCCCGATCTCGCTGCAGACCGACTTCAAGAAGTGCGCGGTCAAGATCGTGAAGGTATAGCGTCGGTCGAG
ACCAAGAACAGGGAGACGCC

Downstream 100 bases:

>100_bases
AACGAACAAAGTGGGTACGCCATGCTCGACCTGATCAAGCGCTACTGGCGGACGATCAACCGGCCCAGCGCCTATTTCAG
CCTGGGTTTCCTGACCCTGG

Product: periplasmic nitrate reductase small subunit

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MKPSRSWASLLAVCAVLLAALAMQAIFFPAPARAQGLVDAMRGPTAIANEPRAPLLYPTENKDIRRTRNYTMQPPTIPHK
IDGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLADVSPRRYFCTQCHVPQADTKPLIGNNFVDVDTILKR
RPGAKGAAK

Sequences:

>Translated_169_residues
MKPSRSWASLLAVCAVLLAALAMQAIFFPAPARAQGLVDAMRGPTAIANEPRAPLLYPTENKDIRRTRNYTMQPPTIPHK
IDGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLADVSPRRYFCTQCHVPQADTKPLIGNNFVDVDTILKR
RPGAKGAAK
>Mature_169_residues
MKPSRSWASLLAVCAVLLAALAMQAIFFPAPARAQGLVDAMRGPTAIANEPRAPLLYPTENKDIRRTRNYTMQPPTIPHK
IDGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLADVSPRRYFCTQCHVPQADTKPLIGNNFVDVDTILKR
RPGAKGAAK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=135, Percent_Identity=42.2222222222222, Blast_Score=111, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPB_CUPNH (P39186)

Other databases:

- EMBL:   X71385
- EMBL:   AY305378
- PIR:   B48489
- RefSeq:   NP_942850.1
- ProteinModelPortal:   P39186
- SMR:   P39186
- GeneID:   2656654
- GenomeReviews:   AY305378_GR
- KEGG:   reh:PHG212
- HOGENOM:   HBG321219
- OMA:   IRRTRNY
- PhylomeDB:   P39186
- ProtClustDB:   CLSK587816
- BioCyc:   REUT381666:PHG212-MONOMER
- GO:   GO:0006810
- InterPro:   IPR011031
- InterPro:   IPR005591

Pfam domain/function: PF03892 NapB

EC number: NA

Molecular weight: Translated: 18924; Mature: 18924

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 93-93 BINDING 96-96 BINDING 133-133 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSRSWASLLAVCAVLLAALAMQAIFFPAPARAQGLVDAMRGPTAIANEPRAPLLYPTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCC
NKDIRRTRNYTMQPPTIPHKIDGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDN
CCHHHHHCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCC
NVLADVSPRRYFCTQCHVPQADTKPLIGNNFVDVDTILKRRPGAKGAAK
CEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKPSRSWASLLAVCAVLLAALAMQAIFFPAPARAQGLVDAMRGPTAIANEPRAPLLYPTE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCC
NKDIRRTRNYTMQPPTIPHKIDGYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDN
CCHHHHHCCCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCEEEEEEEECCCC
NVLADVSPRRYFCTQCHVPQADTKPLIGNNFVDVDTILKRRPGAKGAAK
CEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488