Click here to switch to the map view.

The map label for this gene is hyb0 [C]

Identifier: 38637729

GI number: 38637729

Start: 59962

End: 61017

Strand: Direct

Name: hyb0 [C]

Synonym: PHG064

Alternate gene names: 38637729

Gene position: 59962-61017 (Clockwise)

Preceding gene: 38637723

Following gene: 38637730

Centisome position: 13.26

GC content: 64.39

Gene sequence:

>1056_bases
ATGGCAGAACAGGCCGTTCCCTATGGGCGCAAGACTCAGCACACGCCCGCCCTCAAGGAAGTCCATATCCTCTGGATCAC
CGCCGGTCTTGGCTGCGACGGCGATTCCGTTTCCATTACGGCAGCAAGCCAGCCCAGCGTCGAGGACGTGGTGCTTGGCG
CGATTCCCGGCCTGCCGAAGGTGCATCTGCATAACCCGGTCCTGGCCTATGAAAACGGCGACGAATTCATGGCGCCGTTC
CACAAGGCCGCCCGCGGCGAAATCGATAACTTCGTGCTGGTGCTTGAAGGGTCCATTCCCAATGAGCGCATCAACGGCGA
GGGCTATTGGGCGGCGATGGGCACCGACCCCCAGACCCACCAGCCCATCACGATTCCCGAATGGCTGGACCGGCTCGCGC
CCAAGGCGCTGGCCGTGGTGGGGGCGGGCACTTGCGCCACCTATGGCGGCATCCACGCCATGGAAGGCAATCCAACCGGC
TGCATGGGCCTGGCGGACTACCTCGGCTGGCAGTGGAAATCCCGTGCCGGCCTGCCCATCGTGAACGTCCCGGGCTGCCC
CGTGCAGCCTGATAACTTCATGGAAACGCTGCTGTACCTCTTGTACCAGCTGGCGGGCCTCGCGCCGATGATCCCGCTGG
ACGAGGCGCTGCGCCCGAAATGGCTCTTCACGCGCACCGTGCACGATGGCTGCGATCGCGCCGGCTCGTACGAGCAGGCC
ATCTTCGCCACCGAGTACGGCAACCCGAACTGCATTGTCAAGCTTGGCTGCTGGGGGCCGGTGGTGCAATGCAACGTACC
AAAGCGGGGCTGGATCGCCGGCGTCGGCGGGTGTCCCAACGTGGGCGGGATCTGCATCGGCTGCACCATGCCGGGCTTCC
CGGACAAGTTCATGCCGTTCATGGATGCGCCGCCTGGAGCCGTGCTGTCGTCGAACCTGATCAAGAGCTACGGCCCGCTG
ATTCGCAGCCTGCGCAAGCTGACCAAGGACACGCTCAACGATGAGCCGAAGTGGCGGCACAACCAGCCAGTTCTCACTAC
CGGCTACCGCGGATAA

Upstream 100 bases:

>100_bases
TTGGCGGCGCTTCGCTCGCTTGCTGGATCCGTGGATGCCGTGCATCGATCCCACGGGTCATTGAGAGTCAAAGAACGCGG
TCAGCAGTGCGAGGCAGATC

Downstream 100 bases:

>100_bases
GCGGCGGGAACGCGCATTCTAAGATTTGGAGGTGGTCATGGCCACAATCGCTGCTCCTGGTGCCAGGCCGCGCGCCCAAG
CCGCGCCGGGCAAGCTGGTC

Product: putative [NiFe] hydrogenase small subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MAEQAVPYGRKTQHTPALKEVHILWITAGLGCDGDSVSITAASQPSVEDVVLGAIPGLPKVHLHNPVLAYENGDEFMAPF
HKAARGEIDNFVLVLEGSIPNERINGEGYWAAMGTDPQTHQPITIPEWLDRLAPKALAVVGAGTCATYGGIHAMEGNPTG
CMGLADYLGWQWKSRAGLPIVNVPGCPVQPDNFMETLLYLLYQLAGLAPMIPLDEALRPKWLFTRTVHDGCDRAGSYEQA
IFATEYGNPNCIVKLGCWGPVVQCNVPKRGWIAGVGGCPNVGGICIGCTMPGFPDKFMPFMDAPPGAVLSSNLIKSYGPL
IRSLRKLTKDTLNDEPKWRHNQPVLTTGYRG

Sequences:

>Translated_351_residues
MAEQAVPYGRKTQHTPALKEVHILWITAGLGCDGDSVSITAASQPSVEDVVLGAIPGLPKVHLHNPVLAYENGDEFMAPF
HKAARGEIDNFVLVLEGSIPNERINGEGYWAAMGTDPQTHQPITIPEWLDRLAPKALAVVGAGTCATYGGIHAMEGNPTG
CMGLADYLGWQWKSRAGLPIVNVPGCPVQPDNFMETLLYLLYQLAGLAPMIPLDEALRPKWLFTRTVHDGCDRAGSYEQA
IFATEYGNPNCIVKLGCWGPVVQCNVPKRGWIAGVGGCPNVGGICIGCTMPGFPDKFMPFMDAPPGAVLSSNLIKSYGPL
IRSLRKLTKDTLNDEPKWRHNQPVLTTGYRG
>Mature_350_residues
AEQAVPYGRKTQHTPALKEVHILWITAGLGCDGDSVSITAASQPSVEDVVLGAIPGLPKVHLHNPVLAYENGDEFMAPFH
KAARGEIDNFVLVLEGSIPNERINGEGYWAAMGTDPQTHQPITIPEWLDRLAPKALAVVGAGTCATYGGIHAMEGNPTGC
MGLADYLGWQWKSRAGLPIVNVPGCPVQPDNFMETLLYLLYQLAGLAPMIPLDEALRPKWLFTRTVHDGCDRAGSYEQAI
FATEYGNPNCIVKLGCWGPVVQCNVPKRGWIAGVGGCPNVGGICIGCTMPGFPDKFMPFMDAPPGAVLSSNLIKSYGPLI
RSLRKLTKDTLNDEPKWRHNQPVLTTGYRG

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=278, Percent_Identity=29.4964028776978, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787206, Length=282, Percent_Identity=24.468085106383, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 37899; Mature: 37768

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQAVPYGRKTQHTPALKEVHILWITAGLGCDGDSVSITAASQPSVEDVVLGAIPGLPK
CCCCCCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHEECCCCCCE
VHLHNPVLAYENGDEFMAPFHKAARGEIDNFVLVLEGSIPNERINGEGYWAAMGTDPQTH
EEECCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCEEEEEECCCCCCC
QPITIPEWLDRLAPKALAVVGAGTCATYGGIHAMEGNPTGCMGLADYLGWQWKSRAGLPI
CCEEHHHHHHHHCCHHEEEEECCHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEE
VNVPGCPVQPDNFMETLLYLLYQLAGLAPMIPLDEALRPKWLFTRTVHDGCDRAGSYEQA
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEHHHHHHHHCCCCCCCE
IFATEYGNPNCIVKLGCWGPVVQCNVPKRGWIAGVGGCPNVGGICIGCTMPGFPDKFMPF
EEEEECCCCCEEEEECCCCCEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCHHCCCC
MDAPPGAVLSSNLIKSYGPLIRSLRKLTKDTLNDEPKWRHNQPVLTTGYRG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure 
AEQAVPYGRKTQHTPALKEVHILWITAGLGCDGDSVSITAASQPSVEDVVLGAIPGLPK
CCCCCCCCCCCCCCCCHHEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHEECCCCCCE
VHLHNPVLAYENGDEFMAPFHKAARGEIDNFVLVLEGSIPNERINGEGYWAAMGTDPQTH
EEECCCEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCEECCCEEEEEECCCCCCC
QPITIPEWLDRLAPKALAVVGAGTCATYGGIHAMEGNPTGCMGLADYLGWQWKSRAGLPI
CCEEHHHHHHHHCCHHEEEEECCHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCCCEE
VNVPGCPVQPDNFMETLLYLLYQLAGLAPMIPLDEALRPKWLFTRTVHDGCDRAGSYEQA
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEHHHHHHHHCCCCCCCE
IFATEYGNPNCIVKLGCWGPVVQCNVPKRGWIAGVGGCPNVGGICIGCTMPGFPDKFMPF
EEEEECCCCCEEEEECCCCCEEEECCCCCCCEEECCCCCCCCCEEEECCCCCCCHHCCCC
MDAPPGAVLSSNLIKSYGPLIRSLRKLTKDTLNDEPKWRHNQPVLTTGYRG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]