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The map label for this gene is hyaB [C]

Identifier: 38637730

GI number: 38637730

Start: 61055

End: 62866

Strand: Direct

Name: hyaB [C]

Synonym: PHG065

Alternate gene names: 38637730

Gene position: 61055-62866 (Clockwise)

Preceding gene: 38637729

Following gene: 38637731

Centisome position: 13.5

GC content: 62.97

Gene sequence:

>1812_bases
ATGGCCACAATCGCTGCTCCTGGTGCCAGGCCGCGCGCCCAAGCCGCGCCGGGCAAGCTGGTCGAGATGAACTGGGATCC
GATCACCCGGATCGTCGGCAGCCTCGGCATCTATACCAAGATTGATTTCGAGAACCGCCGCGTGGCCGAATGCTACAGCA
CGTCGTCCATCTTCCGCGGCTACAGCATCTTCATGAAGGGCAAGGATCCGCGCGATTCCCACTTCATCACCAGCCGCATC
TGCGGCATCTGCGGCGACAACCACGCCACCTGCTCGGTCTACGCGCAGAACATGGCCTATGGGGTGAAGCCGCCGCCCAT
CGCGGATTGGATCATCAACCTGGGCGAGGCGGCCGAATACATGTTCGACCACAACATCTTTCAGGACAACCTGGTCGGCG
TGGATTTCTGCGAGCAAATGGTCCGCGAGACCAACCCCGGCGTGTGGGAGAAGGCGAAGACCGCAGAGGCGCCGCACGCG
GCCGAGCATGGCTATCGCACTATTGCGGACATCATGACGGCGCTCAACCCGTTTACCGGGGAGTTCTACCGCGAGACATT
GCTGGTCAGCCGGTATACGCGCGAGATGTTCTGCCTGATGGAAGGACGCCACGTGCACCCGTCCACGCTCTATCCCGGCG
GGGTGGGCACCGTGCCGACCATCCAGCTGTTTACCGACTACATCACGCGCCTGATGAAGTACGTTGAATTCATGAAAAAG
GTGGTGCCGCTGCACGACGACCTGTTCGACTTCTTCTATGAAGCACTGCCGGGCTACGAGGAGGTGGGCCGGCGCCGAAT
CCTGCTCGGCTGCTGGGGATCGTTCCAGGATCCGAACGTCTGCGACTACAACTACCGCACCATGACCAAATGGGGGCGCG
GGATGTTCGTCACGCCCGGCGTGGTGGTCGACGGCGAACTCCTCACGACGGACCTGGTCGACATCAACCTTAATATCCGC
ATCCTGCTCGGCAGTTCGTTTTACCAGGACTGGGATCATGAGGAAACCTCCGTCAAGAACGACCCGCTCGGTAACGCGGT
GGACCGCAAGCACCCGTGGAACCAGACCACGCTGCCGCGCCCGCAGAAGCGCAACTTCGGCGGCAACTACACCTGGGTGA
TGTCGCCGCGGTGGCTGGACAAGCGCACCGGCGACCATCTCGCGCTGGATACCGGCGGCGGGCCGATCGCGCGGCTATGG
GCCACCGCGCTGGCGGGGCTGGTCGATATCGGATACATCAAGTCCACCGGCCACAGTGTCAAGATCTACCTGCCGAGGAC
CGCGCTTAAGCCGGAAGCCGAGTTCGAGTGGAAGATCCCGATGTGGAGCAACGCCATCGAGCGCGATCGGGCTCGCACCT
ACTTCCAGGCTTACTCGGCGGCGGCTGCGCTGTACTTCGCGGAACAGGCCCTGGCCGAGCTGCATGCAGGCCGCACCCGC
ACGTTCACCGATTTCAAGGTGCCCGACGAGGCAATCGGCTGCGGCTTCCATGAGGCGGTACGCGGCGTGCTCTCGCACCA
CCTGGTCATCCGCGACGGCAAGATCGCCAATTATCATCCGTATCCGCCGACCCCATGGAACGCGAGCCCGCGCGACATCT
ACGGCACGCCCGGTCCCTACGAGGACGCGGTGCAGAACACGCCGATCTTCGAAGAGAACGGGCCCGAGAAGTTCAAGGGT
ATCGACATCATGCGCGCGGTGCGCAGCTTCGACCCTTGCCTGCCGTGTGGCGTGCATATGTACGTGGGCAACGGCAAGAC
CATCGAGCAGTCGCATTCTCCGACCTTCGGCGTGATGGCGGGGGCGCACTGA

Upstream 100 bases:

>100_bases
CTCAACGATGAGCCGAAGTGGCGGCACAACCAGCCAGTTCTCACTACCGGCTACCGCGGATAAGCGGCGGGAACGCGCAT
TCTAAGATTTGGAGGTGGTC

Downstream 100 bases:

>100_bases
TGAGCGGTGCCCGCACGGTCCAGGCGCAGCAGCAGCGCATCGAGGCGATGATCGCGGCACTGGAGAGCGTGGACGACAAC
CAGGCCAGCGCACTCGCGAG

Product: putative [NiFe] hydrogenase large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MATIAAPGARPRAQAAPGKLVEMNWDPITRIVGSLGIYTKIDFENRRVAECYSTSSIFRGYSIFMKGKDPRDSHFITSRI
CGICGDNHATCSVYAQNMAYGVKPPPIADWIINLGEAAEYMFDHNIFQDNLVGVDFCEQMVRETNPGVWEKAKTAEAPHA
AEHGYRTIADIMTALNPFTGEFYRETLLVSRYTREMFCLMEGRHVHPSTLYPGGVGTVPTIQLFTDYITRLMKYVEFMKK
VVPLHDDLFDFFYEALPGYEEVGRRRILLGCWGSFQDPNVCDYNYRTMTKWGRGMFVTPGVVVDGELLTTDLVDINLNIR
ILLGSSFYQDWDHEETSVKNDPLGNAVDRKHPWNQTTLPRPQKRNFGGNYTWVMSPRWLDKRTGDHLALDTGGGPIARLW
ATALAGLVDIGYIKSTGHSVKIYLPRTALKPEAEFEWKIPMWSNAIERDRARTYFQAYSAAAALYFAEQALAELHAGRTR
TFTDFKVPDEAIGCGFHEAVRGVLSHHLVIRDGKIANYHPYPPTPWNASPRDIYGTPGPYEDAVQNTPIFEENGPEKFKG
IDIMRAVRSFDPCLPCGVHMYVGNGKTIEQSHSPTFGVMAGAH

Sequences:

>Translated_603_residues
MATIAAPGARPRAQAAPGKLVEMNWDPITRIVGSLGIYTKIDFENRRVAECYSTSSIFRGYSIFMKGKDPRDSHFITSRI
CGICGDNHATCSVYAQNMAYGVKPPPIADWIINLGEAAEYMFDHNIFQDNLVGVDFCEQMVRETNPGVWEKAKTAEAPHA
AEHGYRTIADIMTALNPFTGEFYRETLLVSRYTREMFCLMEGRHVHPSTLYPGGVGTVPTIQLFTDYITRLMKYVEFMKK
VVPLHDDLFDFFYEALPGYEEVGRRRILLGCWGSFQDPNVCDYNYRTMTKWGRGMFVTPGVVVDGELLTTDLVDINLNIR
ILLGSSFYQDWDHEETSVKNDPLGNAVDRKHPWNQTTLPRPQKRNFGGNYTWVMSPRWLDKRTGDHLALDTGGGPIARLW
ATALAGLVDIGYIKSTGHSVKIYLPRTALKPEAEFEWKIPMWSNAIERDRARTYFQAYSAAAALYFAEQALAELHAGRTR
TFTDFKVPDEAIGCGFHEAVRGVLSHHLVIRDGKIANYHPYPPTPWNASPRDIYGTPGPYEDAVQNTPIFEENGPEKFKG
IDIMRAVRSFDPCLPCGVHMYVGNGKTIEQSHSPTFGVMAGAH
>Mature_602_residues
ATIAAPGARPRAQAAPGKLVEMNWDPITRIVGSLGIYTKIDFENRRVAECYSTSSIFRGYSIFMKGKDPRDSHFITSRIC
GICGDNHATCSVYAQNMAYGVKPPPIADWIINLGEAAEYMFDHNIFQDNLVGVDFCEQMVRETNPGVWEKAKTAEAPHAA
EHGYRTIADIMTALNPFTGEFYRETLLVSRYTREMFCLMEGRHVHPSTLYPGGVGTVPTIQLFTDYITRLMKYVEFMKKV
VPLHDDLFDFFYEALPGYEEVGRRRILLGCWGSFQDPNVCDYNYRTMTKWGRGMFVTPGVVVDGELLTTDLVDINLNIRI
LLGSSFYQDWDHEETSVKNDPLGNAVDRKHPWNQTTLPRPQKRNFGGNYTWVMSPRWLDKRTGDHLALDTGGGPIARLWA
TALAGLVDIGYIKSTGHSVKIYLPRTALKPEAEFEWKIPMWSNAIERDRARTYFQAYSAAAALYFAEQALAELHAGRTRT
FTDFKVPDEAIGCGFHEAVRGVLSHHLVIRDGKIANYHPYPPTPWNASPRDIYGTPGPYEDAVQNTPIFEENGPEKFKGI
DIMRAVRSFDPCLPCGVHMYVGNGKTIEQSHSPTFGVMAGAH

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=628, Percent_Identity=26.1146496815287, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1789368, Length=603, Percent_Identity=24.7097844112769, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 67903; Mature: 67772

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIAAPGARPRAQAAPGKLVEMNWDPITRIVGSLGIYTKIDFENRRVAECYSTSSIFRG
CCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC
YSIFMKGKDPRDSHFITSRICGICGDNHATCSVYAQNMAYGVKPPPIADWIINLGEAAEY
EEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHH
MFDHNIFQDNLVGVDFCEQMVRETNPGVWEKAKTAEAPHAAEHGYRTIADIMTALNPFTG
HHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCH
EFYRETLLVSRYTREMFCLMEGRHVHPSTLYPGGVGTVPTIQLFTDYITRLMKYVEFMKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VVPLHDDLFDFFYEALPGYEEVGRRRILLGCWGSFQDPNVCDYNYRTMTKWGRGMFVTPG
HCCCHHHHHHHHHHHCCCHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCEEECCC
VVVDGELLTTDLVDINLNIRILLGSSFYQDWDHEETSVKNDPLGNAVDRKHPWNQTTLPR
EEECCCEEEEEEEEEEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQKRNFGGNYTWVMSPRWLDKRTGDHLALDTGGGPIARLWATALAGLVDIGYIKSTGHSV
CCCCCCCCCEEEEECCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCEE
KIYLPRTALKPEAEFEWKIPMWSNAIERDRARTYFQAYSAAAALYFAEQALAELHAGRTR
EEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TFTDFKVPDEAIGCGFHEAVRGVLSHHLVIRDGKIANYHPYPPTPWNASPRDIYGTPGPY
EEECCCCCHHHHCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EDAVQNTPIFEENGPEKFKGIDIMRAVRSFDPCLPCGVHMYVGNGKTIEQSHSPTFGVMA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCEEEEC
GAH
CCC
>Mature Secondary Structure 
ATIAAPGARPRAQAAPGKLVEMNWDPITRIVGSLGIYTKIDFENRRVAECYSTSSIFRG
CCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC
YSIFMKGKDPRDSHFITSRICGICGDNHATCSVYAQNMAYGVKPPPIADWIINLGEAAEY
EEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHH
MFDHNIFQDNLVGVDFCEQMVRETNPGVWEKAKTAEAPHAAEHGYRTIADIMTALNPFTG
HHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCH
EFYRETLLVSRYTREMFCLMEGRHVHPSTLYPGGVGTVPTIQLFTDYITRLMKYVEFMKK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VVPLHDDLFDFFYEALPGYEEVGRRRILLGCWGSFQDPNVCDYNYRTMTKWGRGMFVTPG
HCCCHHHHHHHHHHHCCCHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHCCCCEEECCC
VVVDGELLTTDLVDINLNIRILLGSSFYQDWDHEETSVKNDPLGNAVDRKHPWNQTTLPR
EEECCCEEEEEEEEEEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PQKRNFGGNYTWVMSPRWLDKRTGDHLALDTGGGPIARLWATALAGLVDIGYIKSTGHSV
CCCCCCCCCEEEEECCCHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCEE
KIYLPRTALKPEAEFEWKIPMWSNAIERDRARTYFQAYSAAAALYFAEQALAELHAGRTR
EEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TFTDFKVPDEAIGCGFHEAVRGVLSHHLVIRDGKIANYHPYPPTPWNASPRDIYGTPGPY
EEECCCCCHHHHCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EDAVQNTPIFEENGPEKFKGIDIMRAVRSFDPCLPCGVHMYVGNGKTIEQSHSPTFGVMA
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCEEEEC
GAH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]