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The map label for this gene is ilvA [H]

Identifier: 37681428

GI number: 37681428

Start: 3335928

End: 3337457

Strand: Direct

Name: ilvA [H]

Synonym: VV3244

Alternate gene names: 37681428

Gene position: 3335928-3337457 (Clockwise)

Preceding gene: 37681427

Following gene: 161486583

Centisome position: 99.45

GC content: 52.61

Gene sequence:

>1530_bases
ATGTCATTAACCCAACAAACTGGCGCAGACTACCTGCGCCAGATACTACGCGCTCCAGTGTATGAAGTGGCAACCGTCAC
CCCTTTGCAAGAGATGCCGCGTTTATCGGCACGTATTGGTAACAATGTGCAGATCAAACGTGAAGACCGCCAGCCGGTGC
ATTCGTTTAAGTTACGTGGCGCTTACAATATGGTGGCCAATCTGTCGCAAGCGCAGAAGGACGCGGGGGTGATTGCTGCC
TCTGCGGGCAACCATGCGCAAGGCATGGCTCTGTCAGGAACGCGCCTTGGCATTGAAACCACCATCGTGATGCCTCGCAC
GACGCCGGATATCAAAGTCGACGCGGTGCGCGGTTTTGGCGGCAAAGTCGTCTTGCACGGCAGCAACTTCGATGAAGCCA
AAGCCGAAGCCGAGCGTCTTTCGCAAGAGAAAGGTTACACTTTTGTCCCACCTTTCGATCATCCTTTAGTGATTGCTGGG
CAAGGCACCATCGGCATGGAAATGCTGCAGCAAAACGGCCATCTCGACCACATTTTCGTCCCTGTTGGGGGCGGTGGTTT
AGCCGCTGGTGTGGCGGTGTTGGTGAAACAGCTGATGCCAGAGATCAAAGTCATTGCGGTGGAACCTGAAGATTCTGCCT
GCCTCAAGGCGGCATTGGATGCAGGTGAGCCTGTGGTGCTGGATCAAGTCAGTATGTTTGCTGATGGTGTGGCGGTAAAA
CGCATTGGCGATGAAACTTTCCGTCTATGCCAGCACTACATTGACGATCACGTCACCGTCTCCAGTGATGAAATCTGCGC
GGCGGTCAAAGATATTTTCGAAGACACCCGTGCGATTGCTGAGCCTTCCGGAGCACTGGCGCTGGCGGGGCTGAAAAAGT
ATGCCGAGAAACACAAACTCAAAGGACACAACTTGGGTACCGTGCTTTCGGGTGCCAATACCAACTTCCACGGTTTACGT
TATGTTTCCGAGCGTTGTGAGCTGGGTGAAAAACGCGAAGGCTTGCTTGCGGTGACCATTCCAGAGCGCAAAGGGGCATT
TTTTGAGTTCTGCCATTTGATTGGTGGCCGTGCGGTGACGGAGTTCAACTACCGTTACAACGACGACTCGCTGGCCAATA
TTTTTGTCGGTGTTCGTCTGCAAAATGGTCAAGAGGAGTTGGATGGTATCATTCGCGACTTACGCGAAGGCGGCTACCCA
GTGGTGGACCTTTCTGAGGATGAAATGGCCAAGCTGCACGTACGCTACATGATTGGCGGTAAACCCTCCAAACCACTCAA
AGAGCGCTTATACAGCTTTGAGTTTCCAGAATACCCAGGCGCACTGCTTAAGTTCCTGAGCACACTGGGCACACACTGGA
ACATTAGCCTATTTAACTACCGCAACCATGGGGCCGATTATGGCCGCGTACTGTGTGGTTTTGAGCTTGATGAGAGTGAT
TTATCCCGCTTTTCGGCGCATTTGCGCGAACTTGGCTACCAATGCAAAGATGTCACGGATAACCCATCCTACCGTTTCTT
TCTTTCCTAA

Upstream 100 bases:

>100_bases
CCAACGCGAAGTGTCGTTTGCGCTGAAAGCCTACGCCAGCATGGCAACCAGTGCCGATAAAGGCGCGGTAAGAGACAAGT
CAAAGCTTGAGGGCTAAGCA

Downstream 100 bases:

>100_bases
TGCTGTTAAAAACGCCCTGTCAGACAGGGCGTTTTGTTTCGCTCAGCAACCAATCTTGATGCAGTTGATGTGAGTTTCCT
AAGTAGTCCACCATCCACTG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSLTQQTGADYLRQILRAPVYEVATVTPLQEMPRLSARIGNNVQIKREDRQPVHSFKLRGAYNMVANLSQAQKDAGVIAA
SAGNHAQGMALSGTRLGIETTIVMPRTTPDIKVDAVRGFGGKVVLHGSNFDEAKAEAERLSQEKGYTFVPPFDHPLVIAG
QGTIGMEMLQQNGHLDHIFVPVGGGGLAAGVAVLVKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAVK
RIGDETFRLCQHYIDDHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKYAEKHKLKGHNLGTVLSGANTNFHGLR
YVSERCELGEKREGLLAVTIPERKGAFFEFCHLIGGRAVTEFNYRYNDDSLANIFVGVRLQNGQEELDGIIRDLREGGYP
VVDLSEDEMAKLHVRYMIGGKPSKPLKERLYSFEFPEYPGALLKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELDESD
LSRFSAHLRELGYQCKDVTDNPSYRFFLS

Sequences:

>Translated_509_residues
MSLTQQTGADYLRQILRAPVYEVATVTPLQEMPRLSARIGNNVQIKREDRQPVHSFKLRGAYNMVANLSQAQKDAGVIAA
SAGNHAQGMALSGTRLGIETTIVMPRTTPDIKVDAVRGFGGKVVLHGSNFDEAKAEAERLSQEKGYTFVPPFDHPLVIAG
QGTIGMEMLQQNGHLDHIFVPVGGGGLAAGVAVLVKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAVK
RIGDETFRLCQHYIDDHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKYAEKHKLKGHNLGTVLSGANTNFHGLR
YVSERCELGEKREGLLAVTIPERKGAFFEFCHLIGGRAVTEFNYRYNDDSLANIFVGVRLQNGQEELDGIIRDLREGGYP
VVDLSEDEMAKLHVRYMIGGKPSKPLKERLYSFEFPEYPGALLKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELDESD
LSRFSAHLRELGYQCKDVTDNPSYRFFLS
>Mature_508_residues
SLTQQTGADYLRQILRAPVYEVATVTPLQEMPRLSARIGNNVQIKREDRQPVHSFKLRGAYNMVANLSQAQKDAGVIAAS
AGNHAQGMALSGTRLGIETTIVMPRTTPDIKVDAVRGFGGKVVLHGSNFDEAKAEAERLSQEKGYTFVPPFDHPLVIAGQ
GTIGMEMLQQNGHLDHIFVPVGGGGLAAGVAVLVKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAVKR
IGDETFRLCQHYIDDHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKYAEKHKLKGHNLGTVLSGANTNFHGLRY
VSERCELGEKREGLLAVTIPERKGAFFEFCHLIGGRAVTEFNYRYNDDSLANIFVGVRLQNGQEELDGIIRDLREGGYPV
VDLSEDEMAKLHVRYMIGGKPSKPLKERLYSFEFPEYPGALLKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELDESDL
SRFSAHLRELGYQCKDVTDNPSYRFFLS

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=296, Percent_Identity=34.4594594594595, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI33469958, Length=314, Percent_Identity=28.9808917197452, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI19923959, Length=273, Percent_Identity=32.2344322344322, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1790207, Length=502, Percent_Identity=70.9163346613546, Blast_Score=722, Evalue=0.0,
Organism=Escherichia coli, GI1789505, Length=295, Percent_Identity=37.9661016949153, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI71991565, Length=329, Percent_Identity=34.0425531914894, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537387, Length=321, Percent_Identity=35.202492211838, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17508781, Length=261, Percent_Identity=33.7164750957854, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320930, Length=521, Percent_Identity=48.5604606525912, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322631, Length=304, Percent_Identity=36.5131578947368, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319788, Length=264, Percent_Identity=26.5151515151515, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355833, Length=315, Percent_Identity=34.6031746031746, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24645328, Length=251, Percent_Identity=32.6693227091633, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 55960; Mature: 55829

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTQQTGADYLRQILRAPVYEVATVTPLQEMPRLSARIGNNVQIKREDRQPVHSFKLRG
CCCCHHCCHHHHHHHHHCCHHHHHCCCCHHHCCHHHHHCCCCEEEEECCCCCCHHEECCC
AYNMVANLSQAQKDAGVIAASAGNHAQGMALSGTRLGIETTIVMPRTTPDIKVDAVRGFG
HHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCEEEEEEECCC
GKVVLHGSNFDEAKAEAERLSQEKGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDHIFV
CEEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEEE
PVGGGGLAAGVAVLVKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAVK
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHHH
RIGDETFRLCQHYIDDHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKYAEKHKL
HCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
KGHNLGTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERKGAFFEFCHLIGGRAVT
CCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
EFNYRYNDDSLANIFVGVRLQNGQEELDGIIRDLREGGYPVVDLSEDEMAKLHVRYMIGG
EEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHEEEEEEEECC
KPSKPLKERLYSFEFPEYPGALLKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELDESD
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCHHH
LSRFSAHLRELGYQCKDVTDNPSYRFFLS
HHHHHHHHHHHCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SLTQQTGADYLRQILRAPVYEVATVTPLQEMPRLSARIGNNVQIKREDRQPVHSFKLRG
CCCHHCCHHHHHHHHHCCHHHHHCCCCHHHCCHHHHHCCCCEEEEECCCCCCHHEECCC
AYNMVANLSQAQKDAGVIAASAGNHAQGMALSGTRLGIETTIVMPRTTPDIKVDAVRGFG
HHHHHHHHHHHHHCCCEEEECCCCCCCCEEECCCEECEEEEEEECCCCCCEEEEEEECCC
GKVVLHGSNFDEAKAEAERLSQEKGYTFVPPFDHPLVIAGQGTIGMEMLQQNGHLDHIFV
CEEEEECCCCCHHHHHHHHHHHHCCCEECCCCCCCEEEECCCCHHHHHHHCCCCEEEEEE
PVGGGGLAAGVAVLVKQLMPEIKVIAVEPEDSACLKAALDAGEPVVLDQVSMFADGVAVK
EECCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEHHHHHHHHCCHHHH
RIGDETFRLCQHYIDDHVTVSSDEICAAVKDIFEDTRAIAEPSGALALAGLKKYAEKHKL
HCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
KGHNLGTVLSGANTNFHGLRYVSERCELGEKREGLLAVTIPERKGAFFEFCHLIGGRAVT
CCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
EFNYRYNDDSLANIFVGVRLQNGQEELDGIIRDLREGGYPVVDLSEDEMAKLHVRYMIGG
EEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHEEEEEEEECC
KPSKPLKERLYSFEFPEYPGALLKFLSTLGTHWNISLFNYRNHGADYGRVLCGFELDESD
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCHHH
LSRFSAHLRELGYQCKDVTDNPSYRFFLS
HHHHHHHHHHHCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]