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The map label for this gene is def

Identifier: 37681409

GI number: 37681409

Start: 3315373

End: 3315981

Strand: Direct

Name: def

Synonym: VV3225

Alternate gene names: 37681409

Gene position: 3315373-3315981 (Clockwise)

Preceding gene: 37681403

Following gene: 37681410

Centisome position: 98.83

GC content: 45.81

Gene sequence:

>609_bases
TTGCTGCTAGGATGCTGTCATTTAGGCTATAAAATGTCTAGAATTGAGCCAACAAGGTTTGTGCTGTTTCGGCACAGTTC
AACATTTCGAGTGCATATGTCTGTATTACAAGTATTAACATTCCCGGATGATCGTCTTCGTACAGTAGCCAAACCGGTTG
AAAAGGTGACACCAGAGATCCAAAAAATCGTCGATGACATGATAGAAACCATGTACGACGAAGAAGGCATTGGTCTGGCG
GCAACGCAAGTCGACATCCATCAACGTATTGTGGTGATTGATATTTCTGAGAGCCGTAACGAGCCGATGGTGTTAATCAA
TCCTGAAATTTTAGAAAAGCGTGGCGAAGATGGCATTGAAGAGGGCTGTTTATCGGTTCCAGGTGCCCGCGCACTCGTGC
CACGCGCCGCTGAAGTCACGGTTAAAGCGTTAGATCGTGATGGCCATGAATTTACCCTCGAAGCCGATGATTTGCTGGCT
ATCTGTATTCAACACGAATTGGATCACCTACAAGGCAAGCTGTTTGTCGATTACCTATCACCGCTAAAGCGCAAGCGTAT
CCAAGACAAGCTAGCCAAAATCAAACGCTTTAACGAGAAACAGCGCTAA

Upstream 100 bases:

>100_bases
TCACACTAAACAACAAGGGTAACAATGCGCGTGAAGCGGCTTGCAGCACTGAGTTCATGCCTTAACTCCTAGATGGATTA
GCGAAGCGACAAATGGGAAA

Downstream 100 bases:

>100_bases
ATCGCGCTGATACCATAAGAAGGAAGGTACCTTGAGCAAGCCTTTACGTATTGTATTTGCAGGTACTCCGGACTTCGCCG
CCCAACATTTGGCGGCGTTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MLLGCCHLGYKMSRIEPTRFVLFRHSSTFRVHMSVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLA
ATQVDIHQRIVVIDISESRNEPMVLINPEILEKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLA
ICIQHELDHLQGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQR

Sequences:

>Translated_202_residues
MLLGCCHLGYKMSRIEPTRFVLFRHSSTFRVHMSVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLA
ATQVDIHQRIVVIDISESRNEPMVLINPEILEKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLA
ICIQHELDHLQGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQR
>Mature_202_residues
MLLGCCHLGYKMSRIEPTRFVLFRHSSTFRVHMSVLQVLTFPDDRLRTVAKPVEKVTPEIQKIVDDMIETMYDEEGIGLA
ATQVDIHQRIVVIDISESRNEPMVLINPEILEKRGEDGIEEGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLA
ICIQHELDHLQGKLFVDYLSPLKRKRIQDKLAKIKRFNEKQR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=158, Percent_Identity=32.9113924050633, Blast_Score=84, Evalue=7e-17,
Organism=Escherichia coli, GI1789682, Length=168, Percent_Identity=72.6190476190476, Blast_Score=249, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24645728, Length=153, Percent_Identity=30.718954248366, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF2_VIBVY (Q7MGK6)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_936018.1
- ProteinModelPortal:   Q7MGK6
- SMR:   Q7MGK6
- STRING:   Q7MGK6
- GeneID:   2626101
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV3225
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- ProtClustDB:   PRK00150
- BioCyc:   VVUL196600:VV3225-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 23099; Mature: 23099

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLGCCHLGYKMSRIEPTRFVLFRHSSTFRVHMSVLQVLTFPDDRLRTVAKPVEKVTPEI
CEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHH
QKIVDDMIETMYDEEGIGLAATQVDIHQRIVVIDISESRNEPMVLINPEILEKRGEDGIE
HHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEEEEECCCCCCCEEEECHHHHHHCCCCCHH
EGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLAICIQHELDHLQGKLFVDYLS
HHHCCCCCCHHHCCCHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
PLKRKRIQDKLAKIKRFNEKQR
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLGCCHLGYKMSRIEPTRFVLFRHSSTFRVHMSVLQVLTFPDDRLRTVAKPVEKVTPEI
CEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHH
QKIVDDMIETMYDEEGIGLAATQVDIHQRIVVIDISESRNEPMVLINPEILEKRGEDGIE
HHHHHHHHHHHHCCCCCCEEEEEEHHHCEEEEEEECCCCCCCEEEECHHHHHHCCCCCHH
EGCLSVPGARALVPRAAEVTVKALDRDGHEFTLEADDLLAICIQHELDHLQGKLFVDYLS
HHHCCCCCCHHHCCCHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
PLKRKRIQDKLAKIKRFNEKQR
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA