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The map label for this gene is prpB

Identifier: 37679713

GI number: 37679713

Start: 1565943

End: 1566839

Strand: Direct

Name: prpB

Synonym: VV1529

Alternate gene names: 37679713

Gene position: 1565943-1566839 (Clockwise)

Preceding gene: 37679712

Following gene: 37679714

Centisome position: 46.68

GC content: 51.73

Gene sequence:

>897_bases
ATGAGCTTAACTCCGGGGGCGAAGTTCAGACTCGCCGTACAACAAAACGATCCGCTGCAAATCGTCGGCACCATCAACCC
TTATTGCGCCATGATGGCCAAACGCCTCGGCCATCAAGCCATTTACCTTTCTGGTGGCGGTATCGCTAATGCGTCTTACG
GCTTGCCGGACTTAGGCATCACCACACTCAACGACGTGCTGGTGGACGTAGAGCGCATCACCAACGCCTGTGATTTGCCT
CTGCTGGTGGACATTGATACTGGTTTTGGCGGCGCATTCAACATCGCGCGCACCATCAAAGCGATGGAAAAAGCCGGCGC
AGCCGCGGTTCACATGGAAGATCAAGTGGCGCAAAAACGTTGTGGTCACCGTCCAAATAAAGCGATTGTTTCTCAGCAAG
AAATGGTCGATCGCGTTAAAGCGGCGGTGGATGCACGAGTCGATGACAACTTTGTGATTATGGCGCGTACCGATGCGCTG
GCGGTGGAAGGGATTGATAGTGCAATTGAGCGTGCGATTGCCTGTGTAGAAGCGGGCGCGGACATGATTTTCCCCGAAGC
GATGAACAAACTTGAGCAGTATCAGCAATTTTCTGCTGCTTTGGCCCAAGCCACGGGCAAACACGTGCCGATATTGGCCA
ACATCACCGAATTTGGTCAAACGCCTTTGTATGGCTGTGAAGAATTAGCGAAATCCAACGTGGATATGGTGCTGTATCCG
TTGAGTGCCTTCCGCGCGATGAACAAAGCGGCGGAAATGGTCTATCGCCACTTGTTGGAAGTGGGCAATCAAGAGGCGTT
AATCGACTCAATGCAGACGCGTAAAGAGCTCTATGACTATCTCAACTATCACGCGTACGAAGACAAGCTCGATCAGCTGT
TTTCAGAAGGAAAATAA

Upstream 100 bases:

>100_bases
TCGAGATGAAGAGTTGTCGGAAATGTTGATGAGAAGACACATCTCCGGCAGCCGAAAATTAATCGAACAACAGATCCTAA
AGGATTAGAGGAAACGTGTT

Downstream 100 bases:

>100_bases
TCCCAACGCGCTTTTTAGATGAGAGCAAAGTAGGGAAGCCCAAGATAAGAAAAACAAAAAACGGAAGTTCAAGAAGAATA
CCGTCGGAATTAACTTAATC

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSLTPGAKFRLAVQQNDPLQIVGTINPYCAMMAKRLGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDVERITNACDLP
LLVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARVDDNFVIMARTDAL
AVEGIDSAIERAIACVEAGADMIFPEAMNKLEQYQQFSAALAQATGKHVPILANITEFGQTPLYGCEELAKSNVDMVLYP
LSAFRAMNKAAEMVYRHLLEVGNQEALIDSMQTRKELYDYLNYHAYEDKLDQLFSEGK

Sequences:

>Translated_298_residues
MSLTPGAKFRLAVQQNDPLQIVGTINPYCAMMAKRLGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDVERITNACDLP
LLVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARVDDNFVIMARTDAL
AVEGIDSAIERAIACVEAGADMIFPEAMNKLEQYQQFSAALAQATGKHVPILANITEFGQTPLYGCEELAKSNVDMVLYP
LSAFRAMNKAAEMVYRHLLEVGNQEALIDSMQTRKELYDYLNYHAYEDKLDQLFSEGK
>Mature_297_residues
SLTPGAKFRLAVQQNDPLQIVGTINPYCAMMAKRLGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDVERITNACDLPL
LVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAAVDARVDDNFVIMARTDALA
VEGIDSAIERAIACVEAGADMIFPEAMNKLEQYQQFSAALAQATGKHVPILANITEFGQTPLYGCEELAKSNVDMVLYPL
SAFRAMNKAAEMVYRHLLEVGNQEALIDSMQTRKELYDYLNYHAYEDKLDQLFSEGK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=300, Percent_Identity=64, Blast_Score=393, Evalue=1e-111,
Organism=Escherichia coli, GI1790445, Length=175, Percent_Identity=34.8571428571429, Blast_Score=77, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32565; Mature: 32433

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTPGAKFRLAVQQNDPLQIVGTINPYCAMMAKRLGHQAIYLSGGGIANASYGLPDLGI
CCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCH
TTLNDVLVDVERITNACDLPLLVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHH
CGHRPNKAIVSQQEMVDRVKAAVDARVDDNFVIMARTDALAVEGIDSAIERAIACVEAGA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
DMIFPEAMNKLEQYQQFSAALAQATGKHVPILANITEFGQTPLYGCEELAKSNVDMVLYP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCCHHHHHHCCCCEEEEC
LSAFRAMNKAAEMVYRHLLEVGNQEALIDSMQTRKELYDYLNYHAYEDKLDQLFSEGK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SLTPGAKFRLAVQQNDPLQIVGTINPYCAMMAKRLGHQAIYLSGGGIANASYGLPDLGI
CCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCH
TTLNDVLVDVERITNACDLPLLVDIDTGFGGAFNIARTIKAMEKAGAAAVHMEDQVAQKR
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHH
CGHRPNKAIVSQQEMVDRVKAAVDARVDDNFVIMARTDALAVEGIDSAIERAIACVEAGA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
DMIFPEAMNKLEQYQQFSAALAQATGKHVPILANITEFGQTPLYGCEELAKSNVDMVLYP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCCCCCCCHHHHHHCCCCEEEEC
LSAFRAMNKAAEMVYRHLLEVGNQEALIDSMQTRKELYDYLNYHAYEDKLDQLFSEGK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]