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The map label for this gene is prpC [H]

Identifier: 37679714

GI number: 37679714

Start: 1566947

End: 1568152

Strand: Direct

Name: prpC [H]

Synonym: VV1530

Alternate gene names: 37679714

Gene position: 1566947-1568152 (Clockwise)

Preceding gene: 37679713

Following gene: 37679715

Centisome position: 46.71

GC content: 50.91

Gene sequence:

>1206_bases
GTGAAACCGTCTGATGATAAACGCTCCGCTAGAGAGCAAAGTCAGACAGAAAAGGAGTTCAAAATGCCTAGTTCTTTAAC
GAAGAAAGGTGAGTTAGGTGGCGCGGGCCTACGTGGTCAAAGCGCTGGAAGCACCGCTTTATGTACTGTCGGTAAAACGG
GGACAGGGTTAACCTATCGTGGTTATGACATCACAGACTTAGCCAACCATGCACAATTTGAAGAAGTGGCACACTTGCTG
TTACGCGGTCACTTGCCAACGCAGCAAGAGTTGGATGCTTACAAAACTCGCCTGATCGGCCTGCGTGGCTTACCGGCAGA
GTTGAAGCAAGCGCTGGAGCTGATCCCGGCGTCTGCGCATCCGATGGATGTGATGCGCACAGGCTGCTCTGTGCTGGGGA
ACTTAGAACAAGAAACCGATTTTTCGCAGCAGCTTGACGCGACAGAGCGCATGCTGGCGCTTTTTCCTGCGATCATCTGT
TACTGGTATCGTTTTAGCCACGATGGCGTGCGTATCAATACGCAAGACCAATCGCAAGATTGCATCGGTGGTTACTTCCT
CAAAATGCTCACGGGCAAAGAGCCCAGCGAACTGCATAAGCAGGTGATGCATTGTTCGCTGATTTTGTATGCAGAGCACG
AGTTTAATGCCTCTACCTTTGCCGCTCGTGTGTGCGCCTCAACGCTGTCTGATATTCATTCTTGTGTGACCGCGGCGATT
GGCACTTTGCGTGGTCCTTTGCATGGTGGTGCTAACGAGGCAGCGATGGAGATGATCGAAAACTGGCAAACACCAGAAGA
GGCGGAAAGCAACATCCTGCGCATGTTGGCCAACAAAGAGAAAATCATGGGCTTTGGTCATGCGATTTATCGCGAAAGCG
ACCCACGTAATGCTCTAATCAAACGTTGGTCAAAAGCCTTGTCAGAAGCGGTGGGCGATACCCATCTTTACGCCGTCTCA
GAACGTGTGGAAGCGGTGATGAAACGCGAGAAAGATCTGTTTTGTAACGCCGATTTCTTCCATGCCTCGGCCTATCACTT
CATGGACATTCCCACCAAGCTGTTTACGCCGATTTTCGTCATGAGTCGCTTAACAGGCTGGACGGCGCATGTGTTTGAAC
AGCGCGCCAATAATCGAATCATTCGTCCAAGTGCTGACTATATTGGCCCAGATCACCAAGATTGGGTGCCGATTGAACAG
CGCTAG

Upstream 100 bases:

>100_bases
GCGCTTTTTAGATGAGAGCAAAGTAGGGAAGCCCAAGATAAGAAAAACAAAAAACGGAAGTTCAAGAAGAATACCGTCGG
AATTAACTTAATCCAATAAC

Downstream 100 bases:

>100_bases
CGCCTAGAGATTGACCTCTCCTCGTCGCGAGGGGAGGTTTCCGCCCTTTTGCATGGGAAGCAAGTCCATGAGCAATAAAA
ACAATACCTTATATCGCAAA

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKPSDDKRSAREQSQTEKEFKMPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAHLL
LRGHLPTQQELDAYKTRLIGLRGLPAELKQALELIPASAHPMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIIC
YWYRFSHDGVRINTQDQSQDCIGGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAI
GTLRGPLHGGANEAAMEMIENWQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVS
ERVEAVMKREKDLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQ
R

Sequences:

>Translated_401_residues
MKPSDDKRSAREQSQTEKEFKMPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAHLL
LRGHLPTQQELDAYKTRLIGLRGLPAELKQALELIPASAHPMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIIC
YWYRFSHDGVRINTQDQSQDCIGGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAI
GTLRGPLHGGANEAAMEMIENWQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVS
ERVEAVMKREKDLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQ
R
>Mature_401_residues
MKPSDDKRSAREQSQTEKEFKMPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYRGYDITDLANHAQFEEVAHLL
LRGHLPTQQELDAYKTRLIGLRGLPAELKQALELIPASAHPMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIIC
YWYRFSHDGVRINTQDQSQDCIGGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAI
GTLRGPLHGGANEAAMEMIENWQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALIKRWSKALSEAVGDTHLYAVS
ERVEAVMKREKDLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQ
R

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=383, Percent_Identity=24.0208877284595, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=51.9021739130435, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786939, Length=362, Percent_Identity=31.2154696132597, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17555174, Length=357, Percent_Identity=22.6890756302521, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319850, Length=390, Percent_Identity=23.0769230769231, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324328, Length=240, Percent_Identity=26.25, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640124, Length=407, Percent_Identity=23.0958230958231, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640126, Length=407, Percent_Identity=23.0958230958231, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356863, Length=362, Percent_Identity=23.2044198895028, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 44869; Mature: 44869

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSDDKRSAREQSQTEKEFKMPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYR
CCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEC
GYDITDLANHAQFEEVAHLLLRGHLPTQQELDAYKTRLIGLRGLPAELKQALELIPASAH
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
PMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIICYWYRFSHDGVRINTQDQSQD
HHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCH
CIGGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAI
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GTLRGPLHGGANEAAMEMIENWQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALI
HHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KRWSKALSEAVGDTHLYAVSERVEAVMKREKDLFCNADFFHASAYHFMDIPTKLFTPIFV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHHHHHHH
MSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQR
HHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPSDDKRSAREQSQTEKEFKMPSSLTKKGELGGAGLRGQSAGSTALCTVGKTGTGLTYR
CCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEC
GYDITDLANHAQFEEVAHLLLRGHLPTQQELDAYKTRLIGLRGLPAELKQALELIPASAH
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
PMDVMRTGCSVLGNLEQETDFSQQLDATERMLALFPAIICYWYRFSHDGVRINTQDQSQD
HHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCH
CIGGYFLKMLTGKEPSELHKQVMHCSLILYAEHEFNASTFAARVCASTLSDIHSCVTAAI
HHHHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GTLRGPLHGGANEAAMEMIENWQTPEEAESNILRMLANKEKIMGFGHAIYRESDPRNALI
HHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KRWSKALSEAVGDTHLYAVSERVEAVMKREKDLFCNADFFHASAYHFMDIPTKLFTPIFV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHHHHHHH
MSRLTGWTAHVFEQRANNRIIRPSADYIGPDHQDWVPIEQR
HHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]