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The map label for this gene is pleD [H]

Identifier: 37679644

GI number: 37679644

Start: 1495447

End: 1496706

Strand: Direct

Name: pleD [H]

Synonym: VV1460

Alternate gene names: 37679644

Gene position: 1495447-1496706 (Clockwise)

Preceding gene: 37679642

Following gene: 37679646

Centisome position: 44.58

GC content: 47.06

Gene sequence:

>1260_bases
GTGTTAGGTGACACGAGAAAACTTGTCATCACACCAGAACGTTTTGCTTATTTTGCCACCGATGATCGGGCAGCAGGCGG
TATTACGACGACGGAAATGGCGATTGATGGCCAAACGATAGATTTAAGCTGTAACATGCAAACGGCTAACTACCCGTGGC
CTTACTGTGGCATTTCGATTTTTACTGACACACTTGAAGCGACTAAAGGTATCGACCTTTCCAACTACCACACCATCCGG
CTCAAACTCAGCTACGACAAAACGGGCGAATCCAATGGTCGATTACGCTTCTATTTGCGCAATTTTAATCCGGCTTATTC
CACCGCAGATAATGAGTACACTTACAAATACAACGGCATGGAGTTCACACCGAGTGCCAGCATGGAAACCATTGAGATCC
CGTTGGCGAACTTGCAAGTCATGACCTGGTGGCTAGCCGACAATAAGATTCCGATTGAACATTCTGCACCAGAATATTCC
AATGTGACACGAATTGAGTTGGCGACAGGCTCAGGAGCGGCAAATGGAGAGCATCATATCCGTATTGAGCGCATTGAGTT
TGAAGGTGCGTATTTAGCTCAGGAAACCCTGTTGTTTGCTTTGTTGCTTTCTTGGGTATTATTGGGACTGGCGTTTTCTT
TGAATGAACTACGTAAAAGTAAACACACCCTAGCTGAGTCTCGTTTTCGCCAAGAGCATTTAAAGAAGATGAATGCGGAT
CTTCGCGCGCAGAATTACGAGTTTGCTGAACTTGCTCACCGCGATGCGTTGACGGGCGCGATGAACCGTCATGCGGTTCG
AACTTGGTTGGAGAAAAAAGCGCGTACGGTACGTTGGGGGCAAGAACAGCTTTCAGTTCTCTATCTGGATCTGGATAAGT
TCAAGCGTGTCAATGACCGTTATGGCCATCAAATGGGAGATGATATCTTACGTGAGTTCGTGTTGGTGGTCGCCAGTTCT
TTAGAAGAAGGTGAACGCTTAGTTCGATGGGGCGGTGAGGAGTTTATCGTCTTTCTTCCAGAAACCACGCTGCAACAAGC
AGTAGAAAAAGCAGAAATGATTCGTAAAAATGTTTCGAATCATCTTTGGGTGCATGGTGGCATGCTTACTTGCAGTATCG
GCGTGGCGCAGATGCAGGAAGAACGGGTAACGGAGTTTCTTGCTCGTGCTGATGACGCACTCTATCAAGCCAAACATCGC
GGCCGTAACCGAGTCGAAGTGAATTACGGTATGCAGAAGATAACTAGCGAATGTGGTTAG

Upstream 100 bases:

>100_bases
TAGTGAGTTGAGTTGTTTTTTGGAGGCTGATGATGAAATGGATACATAAACTTATTTTTGCTCTCGCAATATTACCATCA
GTTTAGTCGCGCTTTATAGT

Downstream 100 bases:

>100_bases
ATCTCGGCAATTGCCTTGAACAGACTAAGCCATCCATACTCGGGTGGCTTTTTTGTTGCTTGTTTTTGTATAGCTTATTG
TTGCTTAGCAAACGTGTCAT

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDGQTIDLSCNMQTANYPWPYCGISIFTDTLEATKGIDLSNYHTIR
LKLSYDKTGESNGRLRFYLRNFNPAYSTADNEYTYKYNGMEFTPSASMETIEIPLANLQVMTWWLADNKIPIEHSAPEYS
NVTRIELATGSGAANGEHHIRIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNELRKSKHTLAESRFRQEHLKKMNAD
LRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTVRWGQEQLSVLYLDLDKFKRVNDRYGHQMGDDILREFVLVVASS
LEEGERLVRWGGEEFIVFLPETTLQQAVEKAEMIRKNVSNHLWVHGGMLTCSIGVAQMQEERVTEFLARADDALYQAKHR
GRNRVEVNYGMQKITSECG

Sequences:

>Translated_419_residues
MLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDGQTIDLSCNMQTANYPWPYCGISIFTDTLEATKGIDLSNYHTIR
LKLSYDKTGESNGRLRFYLRNFNPAYSTADNEYTYKYNGMEFTPSASMETIEIPLANLQVMTWWLADNKIPIEHSAPEYS
NVTRIELATGSGAANGEHHIRIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNELRKSKHTLAESRFRQEHLKKMNAD
LRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTVRWGQEQLSVLYLDLDKFKRVNDRYGHQMGDDILREFVLVVASS
LEEGERLVRWGGEEFIVFLPETTLQQAVEKAEMIRKNVSNHLWVHGGMLTCSIGVAQMQEERVTEFLARADDALYQAKHR
GRNRVEVNYGMQKITSECG
>Mature_419_residues
MLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDGQTIDLSCNMQTANYPWPYCGISIFTDTLEATKGIDLSNYHTIR
LKLSYDKTGESNGRLRFYLRNFNPAYSTADNEYTYKYNGMEFTPSASMETIEIPLANLQVMTWWLADNKIPIEHSAPEYS
NVTRIELATGSGAANGEHHIRIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNELRKSKHTLAESRFRQEHLKKMNAD
LRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTVRWGQEQLSVLYLDLDKFKRVNDRYGHQMGDDILREFVLVVASS
LEEGERLVRWGGEEFIVFLPETTLQQAVEKAEMIRKNVSNHLWVHGGMLTCSIGVAQMQEERVTEFLARADDALYQAKHR
GRNRVEVNYGMQKITSECG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=155, Percent_Identity=40.6451612903226, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=35.9756097560976, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=37.9746835443038, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787541, Length=162, Percent_Identity=35.8024691358025, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=34.0782122905028, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787816, Length=155, Percent_Identity=34.1935483870968, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=33.5403726708075, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787802, Length=228, Percent_Identity=29.3859649122807, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=171, Percent_Identity=32.1637426900585, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=33.7278106508876, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=32.5443786982249, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=27.8145695364238, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=31.5151515151515, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081977, Length=106, Percent_Identity=35.8490566037736, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47920; Mature: 47920

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDGQTIDLSCNMQTANYPWPYCGISI
CCCCCCEEEECHHHEEEEECCCCCCCCCEEEEEEECCEEEEEEEEEECCCCCCCCCCHHH
FTDTLEATKGIDLSNYHTIRLKLSYDKTGESNGRLRFYLRNFNPAYSTADNEYTYKYNGM
HHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCE
EFTPSASMETIEIPLANLQVMTWWLADNKIPIEHSAPEYSNVTRIELATGSGAANGEHHI
EECCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEE
RIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNELRKSKHTLAESRFRQEHLKKMNAD
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTVRWGQEQLSVLYLDLDKFKRVNDR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHHHH
YGHQMGDDILREFVLVVASSLEEGERLVRWGGEEFIVFLPETTLQQAVEKAEMIRKNVSN
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
HLWVHGGMLTCSIGVAQMQEERVTEFLARADDALYQAKHRGRNRVEVNYGMQKITSECG
CEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC
>Mature Secondary Structure
MLGDTRKLVITPERFAYFATDDRAAGGITTTEMAIDGQTIDLSCNMQTANYPWPYCGISI
CCCCCCEEEECHHHEEEEECCCCCCCCCEEEEEEECCEEEEEEEEEECCCCCCCCCCHHH
FTDTLEATKGIDLSNYHTIRLKLSYDKTGESNGRLRFYLRNFNPAYSTADNEYTYKYNGM
HHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCE
EFTPSASMETIEIPLANLQVMTWWLADNKIPIEHSAPEYSNVTRIELATGSGAANGEHHI
EECCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEE
RIERIEFEGAYLAQETLLFALLLSWVLLGLAFSLNELRKSKHTLAESRFRQEHLKKMNAD
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LRAQNYEFAELAHRDALTGAMNRHAVRTWLEKKARTVRWGQEQLSVLYLDLDKFKRVNDR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHHHH
YGHQMGDDILREFVLVVASSLEEGERLVRWGGEEFIVFLPETTLQQAVEKAEMIRKNVSN
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCC
HLWVHGGMLTCSIGVAQMQEERVTEFLARADDALYQAKHRGRNRVEVNYGMQKITSECG
CEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA