Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 37679538

GI number: 37679538

Start: 1380257

End: 1380871

Strand: Direct

Name: hisH

Synonym: VV1354

Alternate gene names: 37679538

Gene position: 1380257-1380871 (Clockwise)

Preceding gene: 37679537

Following gene: 37679539

Centisome position: 41.15

GC content: 47.48

Gene sequence:

>615_bases
GTGACAGAACAGAAAGTTGTCATTATCGATACTGGCTGTGCCAACGTTTCTTCGGTGAAGTTTGCCATTGAACGCCTAGG
CTATGACGTAACGATTTCAAAAGATCCACAAGTGGTATTGTCGGCTGACAAGCTATTCCTACCGGGTGTGGGCACAGCCA
GTGAAGCGATGAAGAACCTAGAAGAGCGCGATCTGATCAGCTTGGTAAAACAAGTAGAGAAGCCGCTGCTCGGTATCTGT
TTAGGCATGCAACTGTTGGGCAAAGTATCTCAAGAGAAAGGGCAGAAAGCCGACGAGTTAGTTGAGTGTCTTGGACTTTG
TGATGGTGAAGTGAAGCTGCTACAAACTGGCGACTTACCGCTGCCGCATATGGGCTGGAACACGGTGTCTGCAAAAGCAG
GTAACCCACTGTTCAAAGGTATTGAAGAAGGTGAGTACTTCTACTTCGTGCACAGCTTTGCAATGCCGGTTGGAGACTAC
ACCATTGCTGAATGTGAATACGGCAACTCGTTCACAGCGGCAGTACAAAGTGGTAACTACTACGGTGTGCAGTTCCATCC
TGAGCGTTCTTCAAAAGCGGGCGCGAAGCTTATTCAAAACTTCTTAGAATTATAA

Upstream 100 bases:

>100_bases
CGTTTGGTCGTACTCTGCGCCAAGCAATCAAAGTAGAAGGCACTGAGCTACCAAGTAGTAAAGGCGTGCTGTAAGGCGAG
CGTAAGCAAGGAGAAAAACA

Downstream 100 bases:

>100_bases
AAGGGATTTAGTGTGATTATTCCAGCTCTTGATTTAATTGAAGGACAGGTGGTTCGCCTTTATCAAGGTGATTACGGCCA
GGTCACCGAATACAAAGTCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit 1; IGP synthase subunit hisH 1; ImGP synthase subunit hisH 1; IGPS subunit hisH 1

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTEQKVVIIDTGCANVSSVKFAIERLGYDVTISKDPQVVLSADKLFLPGVGTASEAMKNLEERDLISLVKQVEKPLLGIC
LGMQLLGKVSQEKGQKADELVECLGLCDGEVKLLQTGDLPLPHMGWNTVSAKAGNPLFKGIEEGEYFYFVHSFAMPVGDY
TIAECEYGNSFTAAVQSGNYYGVQFHPERSSKAGAKLIQNFLEL

Sequences:

>Translated_204_residues
MTEQKVVIIDTGCANVSSVKFAIERLGYDVTISKDPQVVLSADKLFLPGVGTASEAMKNLEERDLISLVKQVEKPLLGIC
LGMQLLGKVSQEKGQKADELVECLGLCDGEVKLLQTGDLPLPHMGWNTVSAKAGNPLFKGIEEGEYFYFVHSFAMPVGDY
TIAECEYGNSFTAAVQSGNYYGVQFHPERSSKAGAKLIQNFLEL
>Mature_203_residues
TEQKVVIIDTGCANVSSVKFAIERLGYDVTISKDPQVVLSADKLFLPGVGTASEAMKNLEERDLISLVKQVEKPLLGICL
GMQLLGKVSQEKGQKADELVECLGLCDGEVKLLQTGDLPLPHMGWNTVSAKAGNPLFKGIEEGEYFYFVHSFAMPVGDYT
IAECEYGNSFTAAVQSGNYYGVQFHPERSSKAGAKLIQNFLEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=61.5, Blast_Score=256, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=34.5794392523364, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS51_VIBVY (Q7MLS3)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_934147.1
- ProteinModelPortal:   Q7MLS3
- SMR:   Q7MLS3
- STRING:   Q7MLS3
- GeneID:   2624158
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV1354
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13170
- BioCyc:   VVUL196600:VV1354-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22216; Mature: 22085

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 80-80 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQKVVIIDTGCANVSSVKFAIERLGYDVTISKDPQVVLSADKLFLPGVGTASEAMKNL
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEEEECCEEEECCCCCHHHHHHCC
EERDLISLVKQVEKPLLGICLGMQLLGKVSQEKGQKADELVECLGLCDGEVKLLQTGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCC
LPHMGWNTVSAKAGNPLFKGIEEGEYFYFVHSFAMPVGDYTIAECEYGNSFTAAVQSGNY
CCCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCEEEEEECCCE
YGVQFHPERSSKAGAKLIQNFLEL
EEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TEQKVVIIDTGCANVSSVKFAIERLGYDVTISKDPQVVLSADKLFLPGVGTASEAMKNL
CCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEEEECCEEEECCCCCHHHHHHCC
EERDLISLVKQVEKPLLGICLGMQLLGKVSQEKGQKADELVECLGLCDGEVKLLQTGDLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCC
LPHMGWNTVSAKAGNPLFKGIEEGEYFYFVHSFAMPVGDYTIAECEYGNSFTAAVQSGNY
CCCCCCCCCCCCCCCHHHCCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCEEEEEECCCE
YGVQFHPERSSKAGAKLIQNFLEL
EEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA