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The map label for this gene is holB [H]

Identifier: 37679465

GI number: 37679465

Start: 1300879

End: 1301835

Strand: Direct

Name: holB [H]

Synonym: VV1281

Alternate gene names: 37679465

Gene position: 1300879-1301835 (Clockwise)

Preceding gene: 37679464

Following gene: 37679466

Centisome position: 38.78

GC content: 45.66

Gene sequence:

>957_bases
TTGACACACTATCCTTGGTTAGCATCGACATGGCAAGGGCTGCAGTCCGCCATGTCGGCACAGCGGTTACCTGGTGCCAT
CTTGTTGCAATCTGACGTTGAGCTCGGTGTTGACGAGCTTATCGAAAAGTTTTCGGCGGCTTTGCTGTGTAAGACATACC
CTGATGAGGGTTGTGGCTTTTGCCATAGTTGCGAGCTGTTAAAGTCGCAAAACCATCCTGATCTTCATTTCATTAGGCCA
GAGAAAGAAGGCAAGGCTATTACTGTTGAGCAGATACGCCAATGTAACCGTCAGGCACAAGAATCTTCTCAAATGGACGG
ATATCGTTTGTTTGTTATCGAACCTGCGGAGGCGATGAACGAATCGGCTTCAAATGCGTTGTTGAAAACATTGGAAGAGC
CGTCTGGGAGCTGCCTATTTTTGTTGATTTGCCACGATAAGAAACGGTTGTTGCCCACTATCATCAGCCGTTGTCAGCAG
TGGAGCATTACGCCTCCTGCGGTCTCGGAGTCAATGCAATGGTTGCAGCAAAAAGGGGTCATCGTGAATGAGTCCTTTTT
GAAACTGGCTCATTACTCGCCCGTGACGGCAATGCACATGGCAAAAGAATCAGAGTTTCAACACTATGAAAAACTCGAAA
GACAGTTTGTGGATTTCTTAATTCAGGCGGATTCCGACTACGTGTCACTATGTCAGACAATTCAAGCCGCGACGTTACGT
AATCTCTCGTGGTTGTGGTTACTGCTTTCTGATGCGCAGAAGGTTCATTTTGGCTTGCAAAATGAAGAAACATTGCAAGG
TAGTCACGCGCTAGGGCAACTGCCTTTGAGCGGATTACAAACCTCAATGGTAAAACTCAATCAATTAAATCAACAGTTAC
ATCAGTTTTCGGGTTTAAACGAAGAACTGATGATCATCAACTGGTTAATCGAAACTAGAGAGGCATTATGTTTGTAG

Upstream 100 bases:

>100_bases
TCTATCGTTACGATTAACGCAGAGCAAAGCATTGAGCAGGTCGCAAACGACATTCGATCTGCGCTCAACCTTTGGCTAGA
ACAGTTACAGGATTAAGGTT

Downstream 100 bases:

>100_bases
ATTCTCATTGTCACCTAGACAAATTGAATTACGATGATCTGCACATAGATGTTGCTGACGTGATCAACAAAGCCAAGCAG
GTAAATGTGGAGCAATTGCT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTHYPWLASTWQGLQSAMSAQRLPGAILLQSDVELGVDELIEKFSAALLCKTYPDEGCGFCHSCELLKSQNHPDLHFIRP
EKEGKAITVEQIRQCNRQAQESSQMDGYRLFVIEPAEAMNESASNALLKTLEEPSGSCLFLLICHDKKRLLPTIISRCQQ
WSITPPAVSESMQWLQQKGVIVNESFLKLAHYSPVTAMHMAKESEFQHYEKLERQFVDFLIQADSDYVSLCQTIQAATLR
NLSWLWLLLSDAQKVHFGLQNEETLQGSHALGQLPLSGLQTSMVKLNQLNQQLHQFSGLNEELMIINWLIETREALCL

Sequences:

>Translated_318_residues
MTHYPWLASTWQGLQSAMSAQRLPGAILLQSDVELGVDELIEKFSAALLCKTYPDEGCGFCHSCELLKSQNHPDLHFIRP
EKEGKAITVEQIRQCNRQAQESSQMDGYRLFVIEPAEAMNESASNALLKTLEEPSGSCLFLLICHDKKRLLPTIISRCQQ
WSITPPAVSESMQWLQQKGVIVNESFLKLAHYSPVTAMHMAKESEFQHYEKLERQFVDFLIQADSDYVSLCQTIQAATLR
NLSWLWLLLSDAQKVHFGLQNEETLQGSHALGQLPLSGLQTSMVKLNQLNQQLHQFSGLNEELMIINWLIETREALCL
>Mature_317_residues
THYPWLASTWQGLQSAMSAQRLPGAILLQSDVELGVDELIEKFSAALLCKTYPDEGCGFCHSCELLKSQNHPDLHFIRPE
KEGKAITVEQIRQCNRQAQESSQMDGYRLFVIEPAEAMNESASNALLKTLEEPSGSCLFLLICHDKKRLLPTIISRCQQW
SITPPAVSESMQWLQQKGVIVNESFLKLAHYSPVTAMHMAKESEFQHYEKLERQFVDFLIQADSDYVSLCQTIQAATLRN
LSWLWLLLSDAQKVHFGLQNEETLQGSHALGQLPLSGLQTSMVKLNQLNQQLHQFSGLNEELMIINWLIETREALCL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=324, Percent_Identity=28.3950617283951, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36075; Mature: 35944

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHYPWLASTWQGLQSAMSAQRLPGAILLQSDVELGVDELIEKFSAALLCKTYPDEGCGF
CCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCCCH
CHSCELLKSQNHPDLHFIRPEKEGKAITVEQIRQCNRQAQESSQMDGYRLFVIEPAEAMN
HHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHC
ESASNALLKTLEEPSGSCLFLLICHDKKRLLPTIISRCQQWSITPPAVSESMQWLQQKGV
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
IVNESFLKLAHYSPVTAMHMAKESEFQHYEKLERQFVDFLIQADSDYVSLCQTIQAATLR
EECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NLSWLWLLLSDAQKVHFGLQNEETLQGSHALGQLPLSGLQTSMVKLNQLNQQLHQFSGLN
CHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EELMIINWLIETREALCL
CHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THYPWLASTWQGLQSAMSAQRLPGAILLQSDVELGVDELIEKFSAALLCKTYPDEGCGF
CCCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCCCH
CHSCELLKSQNHPDLHFIRPEKEGKAITVEQIRQCNRQAQESSQMDGYRLFVIEPAEAMN
HHHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHC
ESASNALLKTLEEPSGSCLFLLICHDKKRLLPTIISRCQQWSITPPAVSESMQWLQQKGV
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
IVNESFLKLAHYSPVTAMHMAKESEFQHYEKLERQFVDFLIQADSDYVSLCQTIQAATLR
EECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NLSWLWLLLSDAQKVHFGLQNEETLQGSHALGQLPLSGLQTSMVKLNQLNQQLHQFSGLN
CHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
EELMIINWLIETREALCL
CHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]