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The map label for this gene is nrfC [H]

Identifier: 37679436

GI number: 37679436

Start: 1271004

End: 1271801

Strand: Direct

Name: nrfC [H]

Synonym: VV1252

Alternate gene names: 37679436

Gene position: 1271004-1271801 (Clockwise)

Preceding gene: 37679435

Following gene: 37679437

Centisome position: 37.89

GC content: 47.74

Gene sequence:

>798_bases
ATGATGTTCATGCCAATAAAGCCAAAGTGTTGGGCTTGGAGCGCAAAGAGAAAATCAAGATGTGTGTGGATTGCCACTCT
GACTTTAACCAGAAAGAAGAGGAGAGGTAATATGAGTTGTTCAAGACGAAATTTCCTTGCTGGTGGTGGCGCGTTGATCA
TGACAACGGGCATTGCTGGCACTTCTATGGTGACTGGGCGCTTAGCATTTGCGGAAGAGGATAAGGGCCAAGCAATTCGT
TATGGCATGATCCACGACGAGACCGCCTGTATCGGATGTACGGCTTGTACCGATGCCTGCCGAGAGGTGAACAAAGTCCC
TGAAGGCGTAAGTCGATTGGAGATCATACGCAGTGAGCCGCACGGTGAGTACCCGAATGTCGATTACCGTTTTACCCGTA
AATCTTGCCAACACTGTGAAAACCCACCTTGCGTTTACGTGTGTCCTACCGGTGCGGCGTACAAAGATGAAAAAACCGGC
ATCGTGGATGTCCACAAAGAAAAATGTGTCGGTTGCGGCTATTGCCTTGCCGCGTGCCCATATCAAGTACGTTTCTTTAA
CCCAGTTGATCACTCGGCCGATAAATGTAATTTTTGTCGTGACACCAATTTGGCGCAAGGCAAGCTACCCGCCTGTGTGG
AATCTTGTCCAACTAAGGCACTGATTTTTGGTGATTTAAATGATCCGCAAAGTGACGTGAATCGAGTATTGGCTGAGAAG
GTCTATTACCGAGACAAAGTGCACTTAGGCACGAAACCCAAATTATTCAAAATCCCACATCAAAAAGGGGAGGTGTAG

Upstream 100 bases:

>100_bases
GCCAATGGTGCTTGTACCAATTGTCATCAACCTCAGGATCTCCAACAAGCCAGTTGGACCCATGATGTGCATGCGAAAAA
TCTAACCTGCTCGAATTGTC

Downstream 100 bases:

>100_bases
CATGAACGGATTTGAATCTGCATTCCACTTTGATTCTCTGGTCTGGGATTGGATCATTGCGATCTATCTTTTCCTTGCCG
GTATGTCTGCCGGGGCGGTG

Product: formate-dependent nitrite reductase complex, Fe-S protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MMFMPIKPKCWAWSAKRKSRCVWIATLTLTRKKRRGNMSCSRRNFLAGGGALIMTTGIAGTSMVTGRLAFAEEDKGQAIR
YGMIHDETACIGCTACTDACREVNKVPEGVSRLEIIRSEPHGEYPNVDYRFTRKSCQHCENPPCVYVCPTGAAYKDEKTG
IVDVHKEKCVGCGYCLAACPYQVRFFNPVDHSADKCNFCRDTNLAQGKLPACVESCPTKALIFGDLNDPQSDVNRVLAEK
VYYRDKVHLGTKPKLFKIPHQKGEV

Sequences:

>Translated_265_residues
MMFMPIKPKCWAWSAKRKSRCVWIATLTLTRKKRRGNMSCSRRNFLAGGGALIMTTGIAGTSMVTGRLAFAEEDKGQAIR
YGMIHDETACIGCTACTDACREVNKVPEGVSRLEIIRSEPHGEYPNVDYRFTRKSCQHCENPPCVYVCPTGAAYKDEKTG
IVDVHKEKCVGCGYCLAACPYQVRFFNPVDHSADKCNFCRDTNLAQGKLPACVESCPTKALIFGDLNDPQSDVNRVLAEK
VYYRDKVHLGTKPKLFKIPHQKGEV
>Mature_265_residues
MMFMPIKPKCWAWSAKRKSRCVWIATLTLTRKKRRGNMSCSRRNFLAGGGALIMTTGIAGTSMVTGRLAFAEEDKGQAIR
YGMIHDETACIGCTACTDACREVNKVPEGVSRLEIIRSEPHGEYPNVDYRFTRKSCQHCENPPCVYVCPTGAAYKDEKTG
IVDVHKEKCVGCGYCLAACPYQVRFFNPVDHSADKCNFCRDTNLAQGKLPACVESCPTKALIFGDLNDPQSDVNRVLAEK
VYYRDKVHLGTKPKLFKIPHQKGEV

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=228, Percent_Identity=61.4035087719298, Blast_Score=297, Evalue=5e-82,
Organism=Escherichia coli, GI226510944, Length=220, Percent_Identity=44.5454545454545, Blast_Score=194, Evalue=4e-51,
Organism=Escherichia coli, GI1789370, Length=258, Percent_Identity=34.1085271317829, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1790326, Length=154, Percent_Identity=35.0649350649351, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787749, Length=154, Percent_Identity=34.4155844155844, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787122, Length=156, Percent_Identity=39.1025641025641, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787872, Length=156, Percent_Identity=37.8205128205128, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787740, Length=114, Percent_Identity=35.0877192982456, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787478, Length=149, Percent_Identity=30.2013422818792, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787963, Length=187, Percent_Identity=26.7379679144385, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789067, Length=160, Percent_Identity=32.5, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI87082179, Length=147, Percent_Identity=31.2925170068027, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788811, Length=148, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082180, Length=140, Percent_Identity=34.2857142857143, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367245, Length=162, Percent_Identity=32.7160493827161, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789079, Length=194, Percent_Identity=29.3814432989691, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29544; Mature: 29544

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
9.8 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
9.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMFMPIKPKCWAWSAKRKSRCVWIATLTLTRKKRRGNMSCSRRNFLAGGGALIMTTGIAG
CCCCCCCCCCEEECCCCCCCEEEEEEEEEEHHHHCCCCCCCCCCEECCCCEEEEEECCCC
TSMVTGRLAFAEEDKGQAIRYGMIHDETACIGCTACTDACREVNKVPEGVSRLEIIRSEP
CCEEEEEEEECCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HGEYPNVDYRFTRKSCQHCENPPCVYVCPTGAAYKDEKTGIVDVHKEKCVGCGYCLAACP
CCCCCCCCEEECHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECHHHHCCCCHHHHCCC
YQVRFFNPVDHSADKCNFCRDTNLAQGKLPACVESCPTKALIFGDLNDPQSDVNRVLAEK
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
VYYRDKVHLGTKPKLFKIPHQKGEV
HHHHCEEECCCCCCEEECCCCCCCC
>Mature Secondary Structure
MMFMPIKPKCWAWSAKRKSRCVWIATLTLTRKKRRGNMSCSRRNFLAGGGALIMTTGIAG
CCCCCCCCCCEEECCCCCCCEEEEEEEEEEHHHHCCCCCCCCCCEECCCCEEEEEECCCC
TSMVTGRLAFAEEDKGQAIRYGMIHDETACIGCTACTDACREVNKVPEGVSRLEIIRSEP
CCEEEEEEEECCCCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HGEYPNVDYRFTRKSCQHCENPPCVYVCPTGAAYKDEKTGIVDVHKEKCVGCGYCLAACP
CCCCCCCCEEECHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEECHHHHCCCCHHHHCCC
YQVRFFNPVDHSADKCNFCRDTNLAQGKLPACVESCPTKALIFGDLNDPQSDVNRVLAEK
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
VYYRDKVHLGTKPKLFKIPHQKGEV
HHHHCEEECCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]