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The map label for this gene is nrfD [H]

Identifier: 37679437

GI number: 37679437

Start: 1271803

End: 1272759

Strand: Direct

Name: nrfD [H]

Synonym: VV1253

Alternate gene names: 37679437

Gene position: 1271803-1272759 (Clockwise)

Preceding gene: 37679436

Following gene: 37679438

Centisome position: 37.91

GC content: 45.14

Gene sequence:

>957_bases
ATGAACGGATTTGAATCTGCATTCCACTTTGATTCTCTGGTCTGGGATTGGATCATTGCGATCTATCTTTTCCTTGCCGG
TATGTCTGCCGGGGCGGTGATGATCACCGTGTACTTAAAACGCAAAGTGATTGTCGGCGATCCAGCACAAAATGGCATTA
TGAAAGCGATGGCTTGGCTGGCGCCGTTTGGCATCATCTCGGGTTTAACGATTTTGATTTTCCACTTAACCAAACCTCTT
GAGTTTTGGAAGATCATGATTTTCTACAATCCAACTTCCGTTATGTCGATGGGGGTTATTCTCTTCCAAGTGTACATGGC
TGTGTTGTTTGTTTGGATTGGGATTATCTTTCAGAAAACGATCGTTGATTTTCTCGGTGATAAGTTGACCTTTGTTGATG
GCTTGTTGACCTGGTTCAAACGTTTTGAAAACAGCATCGAGTTGTTTTTGGCCTTTTTGGCGTTAGTGCTCGCGGCTTAT
ACGGGGTTCCTACTGTCTGCATTGAAAACCTATCCGATGCTCAATAATCCAGTGTTGCCACTGCTGTTTCTTTTCTCCAG
TTTGTCTTCAGGCGCTGCAGCGTGTTTGATGTTCGGTATTTTGGTGTTTAAAGAGTCAGTGAAAGATCCAAGTGTCAGTT
GGGTACATGGTTTTGAGCGTCCTGTGGTCTTGTTTGAGTTGTTTGTATTGGTCACCTTCTTTACCGGGTTGATCTTTGCG
GGTGGACAAGCGGAAATGGCGGCTTGGAATGCTATCTCTGGTGGATTTTGGGCGCAGTGGTTTTGGGTTGGTGTGGTGTT
GATTGGTATGTTGCTACCGCTGACACTCAACTGGTTAGTGCCAGCCGAAGTGAGGCATAAACATGCTTATATTTTTGTTG
TCACGACACTTAGCATGGTTGGCGTTTTGATGCTGCGTACGTTTATTTTGTACGCCGGCCAGATGACGGTTGTCTAA

Upstream 100 bases:

>100_bases
AATCGAGTATTGGCTGAGAAGGTCTATTACCGAGACAAAGTGCACTTAGGCACGAAACCCAAATTATTCAAAATCCCACA
TCAAAAAGGGGAGGTGTAGC

Downstream 100 bases:

>100_bases
TTAAGGAGTATAGATGGAGGCAGAACTGGGACTAATTTGCCTGATTTTGGCGGCAGTCGGCAGTAGTTTCGCAGTGATAC
TGCACGCCATGAATCGACTG

Product: formate-dependent nitrite reductase complex, NrfD protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNGFESAFHFDSLVWDWIIAIYLFLAGMSAGAVMITVYLKRKVIVGDPAQNGIMKAMAWLAPFGIISGLTILIFHLTKPL
EFWKIMIFYNPTSVMSMGVILFQVYMAVLFVWIGIIFQKTIVDFLGDKLTFVDGLLTWFKRFENSIELFLAFLALVLAAY
TGFLLSALKTYPMLNNPVLPLLFLFSSLSSGAAACLMFGILVFKESVKDPSVSWVHGFERPVVLFELFVLVTFFTGLIFA
GGQAEMAAWNAISGGFWAQWFWVGVVLIGMLLPLTLNWLVPAEVRHKHAYIFVVTTLSMVGVLMLRTFILYAGQMTVV

Sequences:

>Translated_318_residues
MNGFESAFHFDSLVWDWIIAIYLFLAGMSAGAVMITVYLKRKVIVGDPAQNGIMKAMAWLAPFGIISGLTILIFHLTKPL
EFWKIMIFYNPTSVMSMGVILFQVYMAVLFVWIGIIFQKTIVDFLGDKLTFVDGLLTWFKRFENSIELFLAFLALVLAAY
TGFLLSALKTYPMLNNPVLPLLFLFSSLSSGAAACLMFGILVFKESVKDPSVSWVHGFERPVVLFELFVLVTFFTGLIFA
GGQAEMAAWNAISGGFWAQWFWVGVVLIGMLLPLTLNWLVPAEVRHKHAYIFVVTTLSMVGVLMLRTFILYAGQMTVV
>Mature_318_residues
MNGFESAFHFDSLVWDWIIAIYLFLAGMSAGAVMITVYLKRKVIVGDPAQNGIMKAMAWLAPFGIISGLTILIFHLTKPL
EFWKIMIFYNPTSVMSMGVILFQVYMAVLFVWIGIIFQKTIVDFLGDKLTFVDGLLTWFKRFENSIELFLAFLALVLAAY
TGFLLSALKTYPMLNNPVLPLLFLFSSLSSGAAACLMFGILVFKESVKDPSVSWVHGFERPVVLFELFVLVTFFTGLIFA
GGQAEMAAWNAISGGFWAQWFWVGVVLIGMLLPLTLNWLVPAEVRHKHAYIFVVTTLSMVGVLMLRTFILYAGQMTVV

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=316, Percent_Identity=46.2025316455696, Blast_Score=248, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 35658; Mature: 35658

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFESAFHFDSLVWDWIIAIYLFLAGMSAGAVMITVYLKRKVIVGDPAQNGIMKAMAWL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHH
APFGIISGLTILIFHLTKPLEFWKIMIFYNPTSVMSMGVILFQVYMAVLFVWIGIIFQKT
HHHHHHHHHHHHHHHHCCHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVDFLGDKLTFVDGLLTWFKRFENSIELFLAFLALVLAAYTGFLLSALKTYPMLNNPVLP
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLFLFSSLSSGAAACLMFGILVFKESVKDPSVSWVHGFERPVVLFELFVLVTFFTGLIFA
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GGQAEMAAWNAISGGFWAQWFWVGVVLIGMLLPLTLNWLVPAEVRHKHAYIFVVTTLSMV
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHH
GVLMLRTFILYAGQMTVV
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNGFESAFHFDSLVWDWIIAIYLFLAGMSAGAVMITVYLKRKVIVGDPAQNGIMKAMAWL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHH
APFGIISGLTILIFHLTKPLEFWKIMIFYNPTSVMSMGVILFQVYMAVLFVWIGIIFQKT
HHHHHHHHHHHHHHHHCCHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVDFLGDKLTFVDGLLTWFKRFENSIELFLAFLALVLAAYTGFLLSALKTYPMLNNPVLP
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLFLFSSLSSGAAACLMFGILVFKESVKDPSVSWVHGFERPVVLFELFVLVTFFTGLIFA
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GGQAEMAAWNAISGGFWAQWFWVGVVLIGMLLPLTLNWLVPAEVRHKHAYIFVVTTLSMV
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHH
GVLMLRTFILYAGQMTVV
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]