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The map label for this gene is flgJ

Identifier: 37679152

GI number: 37679152

Start: 965802

End: 966722

Strand: Direct

Name: flgJ

Synonym: VV0968

Alternate gene names: 37679152

Gene position: 965802-966722 (Clockwise)

Preceding gene: 37679151

Following gene: 37679153

Centisome position: 28.79

GC content: 47.56

Gene sequence:

>921_bases
ATGATTAACAATCCAAACGACATCGGCTTTATTCACGACATTAGCAATCTTGATAAGCTACGTCAGCAAGCCGTGAACGA
TAAAGATGGCGGTGAACAAAAAGCACTAGAAGCGGCGGCAAAACAGTTTGAATCGATTTTTACTTCCATGTTGTTTAAAT
CGATGCGCGAAGCCAATAGTGGTTTTGAATCGGATTTGATGAACAGCCAAAACCAGCTGTTCTATCGCCAAATGTTGGAT
GAACAGATGGCCAGTGAGCTCAGCTCTTCCGGTTCGCTTGGCTTAGCGGACATGATTGTGGCGCAGTTAAGCAGTGGAAA
AGGCATCGACAAAAATGAGTTGGCAATGCGTGAAGCGGGACAAGAAGCGCCACAACGTATGCCAATCAATCGCTCGAAAG
CACGAGAAACCGAGCAGCGCCTGATTGAGTCAGGCCAGCTTGCTCGCAGTGATAAGGCAAGGTTTGATTCACCAGAGTCG
TTCATTACTTCGATGAGACCTTACGCGGAAAGGGCGGCAAAATCCTTAGGGGTTGAACCCTCTCTATTGCTCGCACAAGC
GGCCTTGGAAACAGGCTGGGGGCAGAAAGTGGTGAAAAATGCTCGTGGCAGCAGCAATAACCTCTTCAACATCAAAGCGG
ATCGCAGTTGGGCGGGTGATAAAGTCACCACGCAAACTTTAGAGTTCCACGACAATACGCCAGTGAAGGAAACGGCAGCG
TTTCGTTCATACGATTCGTTTGCCGATAGCTTTAATGATTACGTAGCGTTTTTAAATAACAATCCGCGTTATCAGACCGC
ACTTCAGCACAATGGCGATTCTGAAAGCTTTATTCGCGGTATTCATCGTGCAGGTTATGCGACGGATCCTGAGTACGCCG
ACAAAGTGCTCAAAGTGCAGCAACGTATCGACAACATGTAA

Upstream 100 bases:

>100_bases
GGTGCTGCACCGTCAGATTTGATGGCCATTCTACAAGCGCTTAAACAAGCGGGTGCTATCGAGGGACAACTGATCATCAT
CTGATCGGTTTGGAGGCAAT

Downstream 100 bases:

>100_bases
TCTCCCAACGGCCTACTAAAAGTAGGCCGTTTTTTTACCCTTCCTTAACCCTTAAATCCTTTCTATATCAGTCATGTGTT
TTTGTTGGCATATCTTTTGC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MINNPNDIGFIHDISNLDKLRQQAVNDKDGGEQKALEAAAKQFESIFTSMLFKSMREANSGFESDLMNSQNQLFYRQMLD
EQMASELSSSGSLGLADMIVAQLSSGKGIDKNELAMREAGQEAPQRMPINRSKARETEQRLIESGQLARSDKARFDSPES
FITSMRPYAERAAKSLGVEPSLLLAQAALETGWGQKVVKNARGSSNNLFNIKADRSWAGDKVTTQTLEFHDNTPVKETAA
FRSYDSFADSFNDYVAFLNNNPRYQTALQHNGDSESFIRGIHRAGYATDPEYADKVLKVQQRIDNM

Sequences:

>Translated_306_residues
MINNPNDIGFIHDISNLDKLRQQAVNDKDGGEQKALEAAAKQFESIFTSMLFKSMREANSGFESDLMNSQNQLFYRQMLD
EQMASELSSSGSLGLADMIVAQLSSGKGIDKNELAMREAGQEAPQRMPINRSKARETEQRLIESGQLARSDKARFDSPES
FITSMRPYAERAAKSLGVEPSLLLAQAALETGWGQKVVKNARGSSNNLFNIKADRSWAGDKVTTQTLEFHDNTPVKETAA
FRSYDSFADSFNDYVAFLNNNPRYQTALQHNGDSESFIRGIHRAGYATDPEYADKVLKVQQRIDNM
>Mature_306_residues
MINNPNDIGFIHDISNLDKLRQQAVNDKDGGEQKALEAAAKQFESIFTSMLFKSMREANSGFESDLMNSQNQLFYRQMLD
EQMASELSSSGSLGLADMIVAQLSSGKGIDKNELAMREAGQEAPQRMPINRSKARETEQRLIESGQLARSDKARFDSPES
FITSMRPYAERAAKSLGVEPSLLLAQAALETGWGQKVVKNARGSSNNLFNIKADRSWAGDKVTTQTLEFHDNTPVKETAA
FRSYDSFADSFNDYVAFLNNNPRYQTALQHNGDSESFIRGIHRAGYATDPEYADKVLKVQQRIDNM

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=299, Percent_Identity=32.1070234113712, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 34157; Mature: 34157

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNPNDIGFIHDISNLDKLRQQAVNDKDGGEQKALEAAAKQFESIFTSMLFKSMREANS
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GFESDLMNSQNQLFYRQMLDEQMASELSSSGSLGLADMIVAQLSSGKGIDKNELAMREAG
CHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
QEAPQRMPINRSKARETEQRLIESGQLARSDKARFDSPESFITSMRPYAERAAKSLGVEP
HHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCH
SLLLAQAALETGWGQKVVKNARGSSNNLFNIKADRSWAGDKVTTQTLEFHDNTPVKETAA
HHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHEECCCCCCHHHHHH
FRSYDSFADSFNDYVAFLNNNPRYQTALQHNGDSESFIRGIHRAGYATDPEYADKVLKVQ
HHHHHHHHHHHHHHEEHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
QRIDNM
HHHCCC
>Mature Secondary Structure
MINNPNDIGFIHDISNLDKLRQQAVNDKDGGEQKALEAAAKQFESIFTSMLFKSMREANS
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GFESDLMNSQNQLFYRQMLDEQMASELSSSGSLGLADMIVAQLSSGKGIDKNELAMREAG
CHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
QEAPQRMPINRSKARETEQRLIESGQLARSDKARFDSPESFITSMRPYAERAAKSLGVEP
HHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCH
SLLLAQAALETGWGQKVVKNARGSSNNLFNIKADRSWAGDKVTTQTLEFHDNTPVKETAA
HHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHEECCCCCCHHHHHH
FRSYDSFADSFNDYVAFLNNNPRYQTALQHNGDSESFIRGIHRAGYATDPEYADKVLKVQ
HHHHHHHHHHHHHHEEHHCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
QRIDNM
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]