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The map label for this gene is pepB

Identifier: 37678946

GI number: 37678946

Start: 765933

End: 767231

Strand: Direct

Name: pepB

Synonym: VV0762

Alternate gene names: 37678946

Gene position: 765933-767231 (Clockwise)

Preceding gene: 37678945

Following gene: 37678947

Centisome position: 22.83

GC content: 49.27

Gene sequence:

>1299_bases
ATGTCTACACAGATGTCTGTATTTTTAAGTCACGAAGTCGCTGCTCCACAGTGGGGCGAGAAAGCACTGGTATCATTCAA
TGAGCAGGGCGCTCTTATCCATACAGGCGAAAGTACGGATTTAACTAAAATCCAACGTGCAGCGCGTAAGTTTGATGTGC
AAGGCATCAAGAGCGTGGTGTTGGCTGGTGACGGTTGGGATGTGGAAGCGATTTGGGCTTTCCACCAAGGTTATCGTAAT
CCGAAAAAATACAGCCAACTTGAGTGGGTTGCGCTAGAAGAGAAAGCACAAGCAGAGCTGGAAGCGCGCATTAAAGCCAC
TGAGTTTACTCGCGATATCATTAACAAACCGGCTGAAGAAGTCGCCCCTCGCCAACTTGCGACGATGGCGGCGGAATTTA
TTCGTTCTGTGGCTCCAGAAGGCACTGTGACAGCACGTATCGTCAAAGATAAAGATCTGCTTGCTGAAGGCTGGGAAGGC
ATTTACGCCGTAGGCCGTGGTTCTGATCGCACTTCAGCAATGCTTCAACTCGACTTTAACCCTACGGGTGATGAGAATGC
GCCTGTTTGGGCATGTTTAGTCGGTAAAGGTATTACCTTTGACTCTGGCGGTTACAGTATCAAGGCGTCCAATTTCATGG
ATTCAATGAAAGCGGACATGGGCGGTTCGGGTACGATTACTGGTGGTCTAGGCCTCGCAATCATGCGCGGACTGAACAAG
CGCGTAAAACTGATCCTATGCTGTGCGGAAAATATGATTTCTGGCCGAGCGTTGAAATTGGGTGATGTGATTACCTACAA
AAACGGTAAGACAGTTGAAATCATGAACACCGACGCGGAAGGTCGCCTAGTACTTGCAGATGGCCTGATTTACGCCTCGG
AACAAAATCCAGAGTTGATCATTGATTGCGCGACCTTAACGGGCGCGGCGAAAAATGCGCTAGGTAACGATTACCACGCG
CTACTGACGTTTGACCAAGCACTTGCGCAAGAAGCGCTAAAGTCAGCAGCAGAAGAAAAAGAAGGTTTGTGGCCTCTGCC
ACTAGCAGAATTCCATCGTGAAATGCTGCCATCAAACTTTGCTGACCTGTCTAATATCGGTGGTGGTGACTACTCTCCTG
GTGCCAGCACGGCAGCGGCATTTCTTTCTTACTTTGTGCAAGATTACCAAAAAGGTTGGTTGCACTTTGACTGCTCGGGG
ACTTACCGTAAGTCAGCCAGCGACAAATGGTCTGCGGGTGCGACAGGTATGGGGGTTTGTACTCTGGCCAACCTGTTAGT
GGAACAAGCGAATAAGTAA

Upstream 100 bases:

>100_bases
TTTTTATCAATTTGACATTTCTACCCTACATAATGCTAACATCCGTGAGTTATTCCAATAGAAAGGCGTAGGTAACGCCG
TTATAAGGCAAGGAGAAACC

Downstream 100 bases:

>100_bases
TACTTCACAGCAGCCCTATTGGGCTGCTGACTATAACAACGACATGAAATAAAGGGACACCTTATGGCTCTAGAAAGAAC
ATTTTCGATCATTAAGCCTG

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSTQMSVFLSHEVAAPQWGEKALVSFNEQGALIHTGESTDLTKIQRAARKFDVQGIKSVVLAGDGWDVEAIWAFHQGYRN
PKKYSQLEWVALEEKAQAELEARIKATEFTRDIINKPAEEVAPRQLATMAAEFIRSVAPEGTVTARIVKDKDLLAEGWEG
IYAVGRGSDRTSAMLQLDFNPTGDENAPVWACLVGKGITFDSGGYSIKASNFMDSMKADMGGSGTITGGLGLAIMRGLNK
RVKLILCCAENMISGRALKLGDVITYKNGKTVEIMNTDAEGRLVLADGLIYASEQNPELIIDCATLTGAAKNALGNDYHA
LLTFDQALAQEALKSAAEEKEGLWPLPLAEFHREMLPSNFADLSNIGGGDYSPGASTAAAFLSYFVQDYQKGWLHFDCSG
TYRKSASDKWSAGATGMGVCTLANLLVEQANK

Sequences:

>Translated_432_residues
MSTQMSVFLSHEVAAPQWGEKALVSFNEQGALIHTGESTDLTKIQRAARKFDVQGIKSVVLAGDGWDVEAIWAFHQGYRN
PKKYSQLEWVALEEKAQAELEARIKATEFTRDIINKPAEEVAPRQLATMAAEFIRSVAPEGTVTARIVKDKDLLAEGWEG
IYAVGRGSDRTSAMLQLDFNPTGDENAPVWACLVGKGITFDSGGYSIKASNFMDSMKADMGGSGTITGGLGLAIMRGLNK
RVKLILCCAENMISGRALKLGDVITYKNGKTVEIMNTDAEGRLVLADGLIYASEQNPELIIDCATLTGAAKNALGNDYHA
LLTFDQALAQEALKSAAEEKEGLWPLPLAEFHREMLPSNFADLSNIGGGDYSPGASTAAAFLSYFVQDYQKGWLHFDCSG
TYRKSASDKWSAGATGMGVCTLANLLVEQANK
>Mature_431_residues
STQMSVFLSHEVAAPQWGEKALVSFNEQGALIHTGESTDLTKIQRAARKFDVQGIKSVVLAGDGWDVEAIWAFHQGYRNP
KKYSQLEWVALEEKAQAELEARIKATEFTRDIINKPAEEVAPRQLATMAAEFIRSVAPEGTVTARIVKDKDLLAEGWEGI
YAVGRGSDRTSAMLQLDFNPTGDENAPVWACLVGKGITFDSGGYSIKASNFMDSMKADMGGSGTITGGLGLAIMRGLNKR
VKLILCCAENMISGRALKLGDVITYKNGKTVEIMNTDAEGRLVLADGLIYASEQNPELIIDCATLTGAAKNALGNDYHAL
LTFDQALAQEALKSAAEEKEGLWPLPLAEFHREMLPSNFADLSNIGGGDYSPGASTAAAFLSYFVQDYQKGWLHFDCSGT
YRKSASDKWSAGATGMGVCTLANLLVEQANK

Specific function: Probably plays an important role in intracellular peptide degradation

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family

Homologues:

Organism=Homo sapiens, GI41393561, Length=324, Percent_Identity=37.962962962963, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI47155554, Length=298, Percent_Identity=34.5637583892617, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI87082123, Length=427, Percent_Identity=61.1241217798595, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1790710, Length=320, Percent_Identity=40.625, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17556903, Length=259, Percent_Identity=35.9073359073359, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17565172, Length=361, Percent_Identity=28.2548476454294, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221379063, Length=330, Percent_Identity=32.7272727272727, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI221379062, Length=330, Percent_Identity=32.7272727272727, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21357381, Length=330, Percent_Identity=32.7272727272727, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI161077148, Length=404, Percent_Identity=26.980198019802, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20130057, Length=404, Percent_Identity=26.980198019802, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI21355725, Length=301, Percent_Identity=30.5647840531561, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24661038, Length=344, Percent_Identity=29.3604651162791, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI20129969, Length=369, Percent_Identity=27.6422764227642, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24662227, Length=371, Percent_Identity=27.2237196765499, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922386, Length=371, Percent_Identity=26.6846361185984, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI20129963, Length=336, Percent_Identity=26.4880952380952, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355645, Length=340, Percent_Identity=25, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24662223, Length=340, Percent_Identity=25, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646701, Length=176, Percent_Identity=31.25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24646703, Length=176, Percent_Identity=31.25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21358201, Length=176, Percent_Identity=31.25, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPB_VIBVU (Q8DEZ4)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759427.2
- ProteinModelPortal:   Q8DEZ4
- SMR:   Q8DEZ4
- GeneID:   1177386
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0431
- HOGENOM:   HBG742580
- OMA:   NDDILRM
- ProtClustDB:   PRK05015
- BioCyc:   VVUL216895:VV1_0431-MONOMER
- BRENDA:   3.4.11.23
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00504
- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819
- PANTHER:   PTHR11963:SF3
- PIRSF:   PIRSF036388
- PRINTS:   PR00481

Pfam domain/function: PF00883 Peptidase_M17

EC number: =3.4.11.23

Molecular weight: Translated: 46671; Mature: 46540

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00631 CYTOSOL_AP

Important sites: ACT_SITE 208-208 ACT_SITE 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQMSVFLSHEVAAPQWGEKALVSFNEQGALIHTGESTDLTKIQRAARKFDVQGIKSVV
CCCCEEEEEECCCCCCCCCCHHHEEECCCCCEEECCCCCCHHHHHHHHHHHCHHCCEEEE
LAGDGWDVEAIWAFHQGYRNPKKYSQLEWVALEEKAQAELEARIKATEFTRDIINKPAEE
EECCCCCHHHHHHHHHCCCCCHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VAPRQLATMAAEFIRSVAPEGTVTARIVKDKDLLAEGWEGIYAVGRGSDRTSAMLQLDFN
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCEEEEECCCCCCEEEEEEECC
PTGDENAPVWACLVGKGITFDSGGYSIKASNFMDSMKADMGGSGTITGGLGLAIMRGLNK
CCCCCCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCH
RVKLILCCAENMISGRALKLGDVITYKNGKTVEIMNTDAEGRLVLADGLIYASEQNPELI
HHEEEEEEHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCEEEEECCEEEECCCCCCEE
IDCATLTGAAKNALGNDYHALLTFDQALAQEALKSAAEEKEGLWPLPLAEFHREMLPSNF
EEEEHHCCHHHHHCCCCEEHEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCH
ADLSNIGGGDYSPGASTAAAFLSYFVQDYQKGWLHFDCSGTYRKSASDKWSAGATGMGVC
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHH
TLANLLVEQANK
HHHHHHHHHCCC
>Mature Secondary Structure 
STQMSVFLSHEVAAPQWGEKALVSFNEQGALIHTGESTDLTKIQRAARKFDVQGIKSVV
CCCEEEEEECCCCCCCCCCHHHEEECCCCCEEECCCCCCHHHHHHHHHHHCHHCCEEEE
LAGDGWDVEAIWAFHQGYRNPKKYSQLEWVALEEKAQAELEARIKATEFTRDIINKPAEE
EECCCCCHHHHHHHHHCCCCCHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VAPRQLATMAAEFIRSVAPEGTVTARIVKDKDLLAEGWEGIYAVGRGSDRTSAMLQLDFN
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHCCCCEEEEECCCCCCEEEEEEECC
PTGDENAPVWACLVGKGITFDSGGYSIKASNFMDSMKADMGGSGTITGGLGLAIMRGLNK
CCCCCCCCEEEEEECCCEEECCCCCEEEHHHHHHHHHHCCCCCCEECHHHHHHHHHCCCH
RVKLILCCAENMISGRALKLGDVITYKNGKTVEIMNTDAEGRLVLADGLIYASEQNPELI
HHEEEEEEHHHHCCCCEEEECCEEEECCCCEEEEEECCCCCEEEEECCEEEECCCCCCEE
IDCATLTGAAKNALGNDYHALLTFDQALAQEALKSAAEEKEGLWPLPLAEFHREMLPSNF
EEEEHHCCHHHHHCCCCEEHEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCH
ADLSNIGGGDYSPGASTAAAFLSYFVQDYQKGWLHFDCSGTYRKSASDKWSAGATGMGVC
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHH
TLANLLVEQANK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA