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The map label for this gene is iscS [H]

Identifier: 37678939

GI number: 37678939

Start: 760666

End: 761880

Strand: Direct

Name: iscS [H]

Synonym: VV0755

Alternate gene names: 37678939

Gene position: 760666-761880 (Clockwise)

Preceding gene: 37678938

Following gene: 37678940

Centisome position: 22.68

GC content: 47.24

Gene sequence:

>1215_bases
ATGAAACTGCCTATTTATCTAGACTATTCGGCAACTTGCCCGGTAGATCCACGTGTCGCTGAGAAAATGGTTCAGTACAT
GACGATGGATGGTACCTTTGGTAACCCAGCGTCACGTTCTCATCGCTACGGTTGGCAGGCAGAAGAAGCGGTTGATACCG
CGCGTGAGCAAATCGCAGAATTGTTGAATGCCGACCCTCGTGAAATTGTGTTTACATCTGGTGCAACAGAATCAGATAAC
CTAGCGATCAAAGGCGCAGCTCACTTCTACTCAAAACAAGGTAAGCACGTCATTACTAGCAAAACTGAGCATAAAGCCGT
ACTGGATACTTGCCGTCAGCTTGAGCGTGAAGGTTTTGAAGTGACTTATCTCGAGCCTGAATCAAATGGTCTTATCAGCC
TAAGCAAGCTTGAAGCCGCAATGCGCGACGACACCGTTCTTGTGTCTATTATGCACGTGAACAATGAAATTGGTGTGATT
CAAGATATTGAAGCCATTGGCGAACTTTGCCGTTCTCGTAAAATCATCTTCCACGTCGATGCAGCGCAGTCAGCGGGCAA
AGTGGCGATTGATGTACAGAAGCTAAAAGTAGACTTGATCTCGCTTTCTGCACATAAGATTTACGGTCCGAAAGGTATCG
GCGCGCTTTATGTACGTCGTAAGCCACGTATTCGTCTTGAGGCTCAAATGCATGGTGGTGGTCATGAGCGTGGTTTCCGT
TCAGGTACGCTACCGACTCACCAAATCGTTGGCATGGGTGAAGCTTTCCGCATTGCCAAGCTTGATATGGAAAAAGATTA
CCAACATGCGTTAGCGCTTCGTAATCGTCTGTTAGACGGCGTGAAAGACATGGAAGCGGTGACCATCAACGGCGATCTTG
ACCAGCGTGTACCGCATAACTTGAACATCAGCTTTGCTTTTGTTGAGGGTGAGTCGCTACTGATGTCTCTAAAAGATCTT
GCAGTATCGTCAGGCAGTGCTTGTACTTCAGCGAGTCTAGAGCCTTCTTATGTACTGCGTGCTCTCGGTCTAAATGATGA
GTTAGCACATAGCTCGATTCGTTTCTCTTTTGGTCGTTTTACCACGGAAGAAGAGATCGACTACGCAATTGAACAAATCC
GTGTAGCGGTTGCAAAGCTGCGCGACATGTCTCCTCTATGGGATATGTACAAGGAAGGAATTGATCTCAACACGGTTGAG
TGGGCTCACCACTAA

Upstream 100 bases:

>100_bases
TGCGGTAAATCATGGGTTGGCTAACAAAACAATTAACACCGCGCCCATCGGTGTAAATTTCCGCTCTTAAGCGGCCAAAA
AGTTTACATTGGAGTGAAGA

Downstream 100 bases:

>100_bases
GATCAATTCATAGACAAGTTATAGAGGATTCGAGGTAAATATCATGGCATACAGCGAAAAAGTAATTGATCACTATGAGA
ACCCACGTAACGTTGGTTCG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAELLNADPREIVFTSGATESDN
LAIKGAAHFYSKQGKHVITSKTEHKAVLDTCRQLEREGFEVTYLEPESNGLISLSKLEAAMRDDTVLVSIMHVNNEIGVI
QDIEAIGELCRSRKIIFHVDAAQSAGKVAIDVQKLKVDLISLSAHKIYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKLDMEKDYQHALALRNRLLDGVKDMEAVTINGDLDQRVPHNLNISFAFVEGESLLMSLKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEEEIDYAIEQIRVAVAKLRDMSPLWDMYKEGIDLNTVE
WAHH

Sequences:

>Translated_404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAELLNADPREIVFTSGATESDN
LAIKGAAHFYSKQGKHVITSKTEHKAVLDTCRQLEREGFEVTYLEPESNGLISLSKLEAAMRDDTVLVSIMHVNNEIGVI
QDIEAIGELCRSRKIIFHVDAAQSAGKVAIDVQKLKVDLISLSAHKIYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKLDMEKDYQHALALRNRLLDGVKDMEAVTINGDLDQRVPHNLNISFAFVEGESLLMSLKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEEEIDYAIEQIRVAVAKLRDMSPLWDMYKEGIDLNTVE
WAHH
>Mature_404_residues
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAELLNADPREIVFTSGATESDN
LAIKGAAHFYSKQGKHVITSKTEHKAVLDTCRQLEREGFEVTYLEPESNGLISLSKLEAAMRDDTVLVSIMHVNNEIGVI
QDIEAIGELCRSRKIIFHVDAAQSAGKVAIDVQKLKVDLISLSAHKIYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
SGTLPTHQIVGMGEAFRIAKLDMEKDYQHALALRNRLLDGVKDMEAVTINGDLDQRVPHNLNISFAFVEGESLLMSLKDL
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRFTTEEEIDYAIEQIRVAVAKLRDMSPLWDMYKEGIDLNTVE
WAHH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=57.7114427860697, Blast_Score=508, Evalue=1e-144,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=32.5358851674641, Blast_Score=202, Evalue=6e-52,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=78.7128712871287, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=348, Percent_Identity=25.8620689655172, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=24.9370277078086, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=58.560794044665, Blast_Score=497, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17533177, Length=332, Percent_Identity=32.5301204819277, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=57.1072319201995, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=58.4577114427861, Blast_Score=497, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44958; Mature: 44958

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAE
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGAAHFYSKQGKHVITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPESNGLISLSKLEAAMRDDTVLVSIMHVNNEIGVIQDIEAIGELCRSRKIIFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEE
AAQSAGKVAIDVQKLKVDLISLSAHKIYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCEEEEEEHEEEEEEEECHHEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKLDMEKDYQHALALRNRLLDGVKDMEAVTINGDLDQRVPHN
CCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCC
LNISFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEEEIDYAIEQIRVAVAKLRDMSPLWDMYKEGIDLNTVEWAHH
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLPIYLDYSATCPVDPRVAEKMVQYMTMDGTFGNPASRSHRYGWQAEEAVDTAREQIAE
CCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LLNADPREIVFTSGATESDNLAIKGAAHFYSKQGKHVITSKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHCCCE
VTYLEPESNGLISLSKLEAAMRDDTVLVSIMHVNNEIGVIQDIEAIGELCRSRKIIFHVD
EEEECCCCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEE
AAQSAGKVAIDVQKLKVDLISLSAHKIYGPKGIGALYVRRKPRIRLEAQMHGGGHERGFR
CCCCCCCEEEEEEHEEEEEEEECHHEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCCC
SGTLPTHQIVGMGEAFRIAKLDMEKDYQHALALRNRLLDGVKDMEAVTINGDLDQRVPHN
CCCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCC
LNISFAFVEGESLLMSLKDLAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFSFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHEEECCCC
TTEEEIDYAIEQIRVAVAKLRDMSPLWDMYKEGIDLNTVEWAHH
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA