Click here to switch to the map view.

The map label for this gene is phoB [H]

Identifier: 37678907

GI number: 37678907

Start: 726823

End: 727512

Strand: Direct

Name: phoB [H]

Synonym: VV0723

Alternate gene names: 37678907

Gene position: 726823-727512 (Clockwise)

Preceding gene: 37678903

Following gene: 37678908

Centisome position: 21.67

GC content: 48.26

Gene sequence:

>690_bases
ATGTCTAGAAGGATTCTGGTAGTAGAGGACGAAGCGCCAATTCGCGAAATGCTGTGTTTCGTGCTTGAACAGAAAGGTTA
CCAGGCGGTTGAAGCTGAAGATTATGATACGGCAGTAAACAAACTTGCTGAACCCTTCCCAGATTTAGTATTACTGGATT
GGATGCTGCCAGGTGGCAGTGGCATCAATTTCATTAAGCACATGAAGCGTGAAGAGCTGACGCGTAACATCCCTGTGGTG
ATGTTGACGGCTCGTGGTGAAGAAGAAGACAAAGTTCGTGGCCTTGAAGTCGGTGCGGACGATTACATTACCAAGCCTTT
TTCGCCAAAAGAGTTAGTGGCTCGCCTTAAAGCGGTGATTCGCCGTGTCACGCCAACCGCACTAGAAGATGTGATTGATG
TTCAAGGGCTTAAACTCGATCCAGTTTCTCACCGTGTGACGGCCAATGACGCGCCTGTTGATATGGGGCCAACAGAATTC
AAAATGTTGCATTTCTTTATGACCCACCAAGAGCGCGTGTACAGTCGTGAGCAACTGTTGAACAATGTGTGGGGCACCAA
TGTTTATGTGGAAGATCGCACGGTTGATGTTCACATTCGTCGCCTGCGTAAAGCGCTAGAAGATGCAGGGCATGATAAAC
TGATTCAAACAGTGCGAGGCGCGGGCTATCGTTTCTCAACGAAAGCGTAA

Upstream 100 bases:

>100_bases
GCAAAGGTCACAAAAGTGTCATAATTGAACGTGATAATGCTTTCAGTTGCAAAGGGCTAGGGCCTATAAATTATTCAAAC
TTACATAAGGTTATTCAATT

Downstream 100 bases:

>100_bases
ATACCATGTCGGAGTCGTGAGTGGTTGAAAGATTAACTTGGAAAAAGCTGGCATGGGAGCTGGCTTTTTTTTACACCCCC
TGGGTAATTGTTGGGTGGAT

Product: DNA-binding response regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLAEPFPDLVLLDWMLPGGSGINFIKHMKREELTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDAPVDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA

Sequences:

>Translated_229_residues
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLAEPFPDLVLLDWMLPGGSGINFIKHMKREELTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDAPVDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA
>Mature_228_residues
SRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLAEPFPDLVLLDWMLPGGSGINFIKHMKREELTRNIPVVM
LTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDAPVDMGPTEFK
MLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=80.7017543859649, Blast_Score=399, Evalue=1e-113,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=38.1578947368421, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.5, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=35.8744394618834, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=35.8744394618834, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=35, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=37.0535714285714, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.8888888888889, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=36.3636363636364, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=37.117903930131, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=32.7272727272727, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=30.8695652173913, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=30.2521008403361, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=40.3669724770642, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788191, Length=121, Percent_Identity=29.7520661157025, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26146; Mature: 26015

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLAEPFPDLVLLDWMLPGGS
CCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
GINFIKHMKREELTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVI
CCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVTPTALEDVIDVQGLKLDPVSHRVTANDAPVDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCC
>Mature Secondary Structure 
SRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDTAVNKLAEPFPDLVLLDWMLPGGS
CCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCC
GINFIKHMKREELTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVI
CCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRVTPTALEDVIDVQGLKLDPVSHRVTANDAPVDMGPTEFKMLHFFMTHQERVYSREQLL
HHCCHHHHHHHHHHCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA
HHHCCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]