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The map label for this gene is lysS

Identifier: 37678853

GI number: 37678853

Start: 665787

End: 667319

Strand: Direct

Name: lysS

Synonym: VV0669

Alternate gene names: 37678853

Gene position: 665787-667319 (Clockwise)

Preceding gene: 37678852

Following gene: 37678854

Centisome position: 19.85

GC content: 47.62

Gene sequence:

>1533_bases
ATGACTGATGCTGTTCAAAACGAAACTGTACAAGAAGCCTCTGCACAAGAAGAGAATAAGCTCATTGCTGAACGCCGCGC
AAAATTGGATCAAATCCGAAAGAGCTGCAAAGCAAATGGCCACCCAAACGACTTCCGTCGTGACAGCCTAGCTGGCGACC
TTCAAAAAGAGTTCGGTGAAAAGAGTAAGGAAGAGCTAGAAGCACTTAACCACGTTGTTGCTATTGCGGGTCGTGTTATG
GCGAAACGTGGTCCATTCTTGGTGATCCAAGAAACTTCTGGTCGTATCCAGGCTTACGCGGACAAAGATGTACAAAAAGT
ATTGAAAGACAAATACCAAGGTCTTGATATCGGTGACATCATCGGTGTGAAAGGTGCGCTGCACAAATCTGGCAAAGGCG
ACCTATACGTAAACATGGAAGAGTACGAGTTGCTAACTAAAGCACTTCGTCCACTGCCAGAGAAGTTCCACGGTCTCACT
GACCAAGAAATGCGTTACCGTCAGCGTTATGTTGACCTCATCGTGAATGAAGATTCTCGTAATGCGTTTGTAGTACGTTC
AAAAGTGATGTCGGCAATCCGTAATTTCATGATCTCTAAACAGTTCATGGAAGTTGAAACGCCAATGATGCACGTGATCC
CTGGCGGTGCGAGTGCGCGTCCATTCATCACGCATCACAATGCGTTAGACATGCCAATGTACCTACGTATTGCGCCAGAG
CTATACCTGAAGCGTCTAGTTGTTGGTGGTTTTGATCGCGTATTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTTTC
TCCACGTCACAACCCTGAATTCACTATGATGGAATTCTACATGGCGTACGCAGACTATAAAGATCTGATGGACCTAACTG
AAGAGCTACTAAGCTCGGTGGCTTTAGAAGTTCTAGGTTCAACGTCAATGCCTTACGGTGAGCATACTGTTGAGTTTGGT
GGCACATACACTCGCATGAGCATGTTTGAAGCAATCAAGCACTACAACCCAGACCACGCGCAAATCCAAGCGCTAACGGA
AGAAGACATTCAAAACCGTGACCTAATGGTTTCTATCGCGAAATCGGTTCACGTAGAAGTCGAACCTTTCTGGACATGTG
GTCAGCTTCTTGAAGAGATCTTTGGTGAAACAGCAGAACCTAAGCTAATGCAGCCAACGTTCATCACTGGCTACCCAGCA
GATATCTCTCCACTGGCTCGTCGTAGCGATGACAACCCGTTCTTCACAGACCGCTTTGAGTTCTTCATCGGTGGCCGTGA
AGTGGCGAACGGTTTCTCTGAGCTTAACGATGCAGAAGACCAAGACGCACGTTTCAAAGCACAGGTTGAAGCGAAAGAGT
CGGGTGATGACGAAGCCATGTTCTACGATGCAGACTACATCACAGCACTAGAGCACGGCCTACCACCGACTGCTGGTCAA
GGTATCGGCATCGACCGTCTAGTGATGCTACTGACCAACACGCACACGATTCGTGACGTTATTCTATTCCCAGCGATGCG
TCCTCAGGCGTAA

Upstream 100 bases:

>100_bases
ATACTCAAGCGGTTCTTGACGGTGATCTGGACAAATTTATTGAAGCTAGCCTGAAATCAGGCCTTTAAGCTTTTCACCGA
CTAAACAGGATACATCGAAA

Downstream 100 bases:

>100_bases
GAGCAAATAGATTGAAAAACCACCTTCGGGTGGTTTTTTTTATCGAGCAGAATTCCTTTTACATCACTAAATGCTACCTG
CATTCCAAGTATTAGTAATT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTDAVQNETVQEASAQEENKLIAERRAKLDQIRKSCKANGHPNDFRRDSLAGDLQKEFGEKSKEELEALNHVVAIAGRVM
AKRGPFLVIQETSGRIQAYADKDVQKVLKDKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEYELLTKALRPLPEKFHGLT
DQEMRYRQRYVDLIVNEDSRNAFVVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPE
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSVALEVLGSTSMPYGEHTVEFG
GTYTRMSMFEAIKHYNPDHAQIQALTEEDIQNRDLMVSIAKSVHVEVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPA
DISPLARRSDDNPFFTDRFEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTAGQ
GIGIDRLVMLLTNTHTIRDVILFPAMRPQA

Sequences:

>Translated_510_residues
MTDAVQNETVQEASAQEENKLIAERRAKLDQIRKSCKANGHPNDFRRDSLAGDLQKEFGEKSKEELEALNHVVAIAGRVM
AKRGPFLVIQETSGRIQAYADKDVQKVLKDKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEYELLTKALRPLPEKFHGLT
DQEMRYRQRYVDLIVNEDSRNAFVVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPE
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSVALEVLGSTSMPYGEHTVEFG
GTYTRMSMFEAIKHYNPDHAQIQALTEEDIQNRDLMVSIAKSVHVEVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPA
DISPLARRSDDNPFFTDRFEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTAGQ
GIGIDRLVMLLTNTHTIRDVILFPAMRPQA
>Mature_509_residues
TDAVQNETVQEASAQEENKLIAERRAKLDQIRKSCKANGHPNDFRRDSLAGDLQKEFGEKSKEELEALNHVVAIAGRVMA
KRGPFLVIQETSGRIQAYADKDVQKVLKDKYQGLDIGDIIGVKGALHKSGKGDLYVNMEEYELLTKALRPLPEKFHGLTD
QEMRYRQRYVDLIVNEDSRNAFVVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPEL
YLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSVALEVLGSTSMPYGEHTVEFGG
TYTRMSMFEAIKHYNPDHAQIQALTEEDIQNRDLMVSIAKSVHVEVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPAD
ISPLARRSDDNPFFTDRFEFFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTAGQG
IGIDRLVMLLTNTHTIRDVILFPAMRPQA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=496, Percent_Identity=42.9435483870968, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI5031815, Length=496, Percent_Identity=42.9435483870968, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI40789249, Length=248, Percent_Identity=26.2096774193548, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1790571, Length=510, Percent_Identity=60.5882352941176, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=496, Percent_Identity=61.6935483870968, Blast_Score=623, Evalue=1e-179,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=30.7228915662651, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788173, Length=292, Percent_Identity=26.7123287671233, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=507, Percent_Identity=41.025641025641, Blast_Score=367, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535927, Length=507, Percent_Identity=41.025641025641, Blast_Score=366, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71994340, Length=507, Percent_Identity=41.025641025641, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32566633, Length=249, Percent_Identity=29.3172690763052, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=42.1875, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=381, Percent_Identity=31.2335958005249, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323011, Length=327, Percent_Identity=25.9938837920489, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325153, Length=326, Percent_Identity=24.2331288343558, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640849, Length=523, Percent_Identity=40.9177820267686, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=523, Percent_Identity=40.9177820267686, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584738, Length=305, Percent_Identity=26.8852459016393, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57893; Mature: 57761

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAVQNETVQEASAQEENKLIAERRAKLDQIRKSCKANGHPNDFRRDSLAGDLQKEFGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
KSKEELEALNHVVAIAGRVMAKRGPFLVIQETSGRIQAYADKDVQKVLKDKYQGLDIGDI
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCCHHHH
IGVKGALHKSGKGDLYVNMEEYELLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEDSR
HCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHEECCCCC
NAFVVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHHHCCHH
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSV
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALEVLGSTSMPYGEHTVEFGGTYTRMSMFEAIKHYNPDHAQIQALTEEDIQNRDLMVSIA
HHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEEH
KSVHVEVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDDNPFFTDRFE
HHHEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCHHH
FFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTAGQ
HEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCCCCC
GIGIDRLVMLLTNTHTIRDVILFPAMRPQA
CCCHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDAVQNETVQEASAQEENKLIAERRAKLDQIRKSCKANGHPNDFRRDSLAGDLQKEFGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
KSKEELEALNHVVAIAGRVMAKRGPFLVIQETSGRIQAYADKDVQKVLKDKYQGLDIGDI
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCCHHHH
IGVKGALHKSGKGDLYVNMEEYELLTKALRPLPEKFHGLTDQEMRYRQRYVDLIVNEDSR
HCCCHHHCCCCCCCEEEEHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHEECCCCC
NAFVVRSKVMSAIRNFMISKQFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPE
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHHHCCHH
LYLKRLVVGGFDRVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSV
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALEVLGSTSMPYGEHTVEFGGTYTRMSMFEAIKHYNPDHAQIQALTEEDIQNRDLMVSIA
HHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHEEEH
KSVHVEVEPFWTCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDDNPFFTDRFE
HHHEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCCCCCCHHH
FFIGGREVANGFSELNDAEDQDARFKAQVEAKESGDDEAMFYDADYITALEHGLPPTAGQ
HEECCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHHHHCCCCCCCC
GIGIDRLVMLLTNTHTIRDVILFPAMRPQA
CCCHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]