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The map label for this gene is prfB [H]

Identifier: 37678852

GI number: 37678852

Start: 664885

End: 665754

Strand: Direct

Name: prfB [H]

Synonym: VV0668

Alternate gene names: 37678852

Gene position: 664885-665754 (Clockwise)

Preceding gene: 37678851

Following gene: 37678853

Centisome position: 19.82

GC content: 47.93

Gene sequence:

>870_bases
GTGGATGACGTTGAAGGTTTGCTTGAGCTGGCCATTGAAGCTGAAGATCAAGAGACCTTTGATGAAATCGGTCCAGAACT
TGAAGCCTTAGAAGAGAAGCTCGCGGGCTTAGAGTTCCGTCGCATGTTCTCTGGCGCACACGATTCTTCAGATTGCTATA
TTGATCTGCAAGCAGGTTCTGGCGGTACAGAAGCGCAAGACTGGACGAGCATGATGCTTCGCATGTATCTTCGTTGGGCT
GAAGCGAAAGGCTTCAAAACCGAAGTGATCGAAGTCTCCGAAGGAGATGTTGCTGGCCTTAAGTCGGTCACCGTACGTAT
TATCGGTGATTATGCTTATGGTTGGTTGCGTACCGAAACGGGTGTTCACCGTTTAGTGCGTAAATCTCCGTTTGACTCAG
GCGGTCGTCGTCACACTTCTTTTGCTTCTGCGTTTGTTTACCCAGAGATTGATGAAAACATCGACATCGACATTAACCCT
GCCGATCTCCGTATTGACGTGTATCGTGCGTCTGGTGCGGGTGGTCAGCACGTAAACACCACCGAATCTGCGGTACGTAT
TACCCACGTACCAACGAATACCGTGGTTCAGTGTCAGAACGACCGTTCTCAACACAAAAACAAAGATCAAGCGATGAAGC
AGCTTCGCGCTAAGCTGTTTGAACTTGAGTTGCAAAAGCAAAATGCGGAAAAACAAGCTAATGAAGACGCTAAGTCTGAC
ATTGGCTGGGGTAGCCAGATCCGTTCTTACGTATTGGATGATTCACGCATTAAAGACCTCCGTACGGGCGTCGAAAACCG
TAATACTCAAGCGGTTCTTGACGGTGATCTGGACAAATTTATTGAAGCTAGCCTGAAATCAGGCCTTTAA

Upstream 100 bases:

>100_bases
ACCGGACGTTTGGAACGAGCCTGATCGAGCGCAAGCTCTCGGTCGTGAACGTGCGTCACTGGAAGCGGTTGTTGAAACCA
TTGATCAACTTGATCAAGGT

Downstream 100 bases:

>100_bases
GCTTTTCACCGACTAAACAGGATACATCGAAAATGACTGATGCTGTTCAAAACGAAACTGTACAAGAAGCCTCTGCACAA
GAAGAGAATAAGCTCATTGC

Product: protein chain release factor B

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDDVEGLLELAIEAEDQETFDEIGPELEALEEKLAGLEFRRMFSGAHDSSDCYIDLQAGSGGTEAQDWTSMMLRMYLRWA
EAKGFKTEVIEVSEGDVAGLKSVTVRIIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFVYPEIDENIDIDINP
ADLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAEKQANEDAKSD
IGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKSGL

Sequences:

>Translated_289_residues
MDDVEGLLELAIEAEDQETFDEIGPELEALEEKLAGLEFRRMFSGAHDSSDCYIDLQAGSGGTEAQDWTSMMLRMYLRWA
EAKGFKTEVIEVSEGDVAGLKSVTVRIIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFVYPEIDENIDIDINP
ADLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAEKQANEDAKSD
IGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKSGL
>Mature_289_residues
MDDVEGLLELAIEAEDQETFDEIGPELEALEEKLAGLEFRRMFSGAHDSSDCYIDLQAGSGGTEAQDWTSMMLRMYLRWA
EAKGFKTEVIEVSEGDVAGLKSVTVRIIGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFASAFVYPEIDENIDIDINP
ADLRIDVYRASGAGGQHVNTTESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAEKQANEDAKSD
IGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=239, Percent_Identity=39.7489539748954, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI34577120, Length=304, Percent_Identity=33.2236842105263, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI166795305, Length=146, Percent_Identity=44.5205479452055, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI2367172, Length=289, Percent_Identity=82.3529411764706, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1787462, Length=240, Percent_Identity=41.6666666666667, Blast_Score=193, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=217, Percent_Identity=35.9447004608295, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=300, Percent_Identity=34.6666666666667, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19921226, Length=248, Percent_Identity=37.9032258064516, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 32293; Mature: 32293

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDVEGLLELAIEAEDQETFDEIGPELEALEEKLAGLEFRRMFSGAHDSSDCYIDLQAGS
CCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECCC
GGTEAQDWTSMMLRMYLRWAEAKGFKTEVIEVSEGDVAGLKSVTVRIIGDYAYGWLRTET
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHEEEEEEECHHHHEEECHH
GVHRLVRKSPFDSGGRRHTSFASAFVYPEIDENIDIDINPADLRIDVYRASGAGGQHVNT
HHHHHHHCCCCCCCCCCHHHHHHEEECCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCC
TESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAEKQANEDAKSD
CCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
IGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKSGL
CCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MDDVEGLLELAIEAEDQETFDEIGPELEALEEKLAGLEFRRMFSGAHDSSDCYIDLQAGS
CCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEEECCC
GGTEAQDWTSMMLRMYLRWAEAKGFKTEVIEVSEGDVAGLKSVTVRIIGDYAYGWLRTET
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHEEEEEEECHHHHEEECHH
GVHRLVRKSPFDSGGRRHTSFASAFVYPEIDENIDIDINPADLRIDVYRASGAGGQHVNT
HHHHHHHCCCCCCCCCCHHHHHHEEECCCCCCCCEEEECCCCEEEEEEEECCCCCCCCCC
TESAVRITHVPTNTVVQCQNDRSQHKNKDQAMKQLRAKLFELELQKQNAEKQANEDAKSD
CCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
IGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKSGL
CCCHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA