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The map label for this gene is braB [H]

Identifier: 37678847

GI number: 37678847

Start: 658889

End: 660202

Strand: Direct

Name: braB [H]

Synonym: VV0663

Alternate gene names: 37678847

Gene position: 658889-660202 (Clockwise)

Preceding gene: 37678846

Following gene: 161486657

Centisome position: 19.64

GC content: 49.01

Gene sequence:

>1314_bases
GTGAATCAGACTTTAAAACTAACCGATATTATCGCAGTCGGCTTTATGCTGTTTGCGTTTTTCTTGGGAGCGGGCAACAT
TATTTTCCCTCCTCTAGCAGGTCAACTTGCGGGTGAGCATCTGTTCCCTGCGATGTCAGGCTTCCTTGTGACGGCAGTAG
GTCTTCCTTTGATCACCATTATTGCCATTGCCGTTGCTGGTGGATCTTGGGATCACTTAACCAAAGATCTTCCTAAACAA
GCAGCAACATTGATGGCGGTATTGATTTTTATCATCATCGGTCCTGCGTTTGCCGCGCCACGTACTGGCCTAGTAGCCTA
CGAAATGGCGGTGAAGCCTTTCTTTGTTGATGCGCTTCAGTCGCATTTGACCATTTTCTCGATCCTTTTCTTTGCGGTGG
CGATGTTCTTTTCTTGGTCGCAAGGCAAACTGATTGACATCATTGGTAAGGTATTAACACCGGCGTTGTTTGTTGGTCTG
ATTATTTTGGCGCTTGCGGTATTTATTGATCCTCAGGGCGAAATGCTTGCTGCGCAAGGTGAGTATCTAACACAGCCACT
CACCAAAGGTTTCCTTGAAGGTTACAACACCATGGATACGTTTGGTTCACTGATGTTCGGTATGCTGATCGTTGATGCGC
TGCGCAGCCGTGGTATTACGGAGCGTTCTGCAACGACTAAATACCTGATTTACGCTGGTTTCATTGCTGCAGCCGGTCTG
GCATTTGTTTACATCTCATTGTTCTACCTTGGCGCAACGAGCGCGGCGGTGGCCTCTGGTGCGGACAACGGTGGCGTGGT
TCTCAGCCTATACGTTCAAGCGCTGTTTGGTCCTTATGGTCAAATCGTGTTGTCTGTAATTGTACTGCTTGCCTGTTTAA
CCACTGCGATTGGTCTGATCTCAGCGTGTTCAGATTACTTCAGCTCACTGACTCCGCTTTCATACAAAGCGTGGGTTGTG
ATCAATGGCGTTGCTTGTGCTGTGGTGGCGAATGTTGGTCTTGCACAGCTTATCGCTCTGTCTGTGCCGGTCCTGTTTGC
ACTATACCCAGTGGCCATTGCGTTGGTGGCACTGACTTTCGTGCGTAAGAAATTGCCAAACCCACAAGTGGCGTACCGCG
TGGTTATCCTTGTTTCACTGCTGTTTGCCTTGATTGATGCAGCAAAAGTGGCGGGCGTGGATGTGTCTGCATTCAAAGTA
CTGCCGCTATTTGAAGTGGGCATGGCGTGGTTGCTACCAACCATGACGGCGATTGTGTGTATGTTCTTTGTCAGCCGCTC
TGTTCGTCAAGAACTGGCTGAAGAAGCCGCTTAA

Upstream 100 bases:

>100_bases
TCATTTCGTCATTATGCGCCCCTAAAATTAGCACAATCGTCGGTCGCAAGAGCGGCGAATGCATACACAACATCATTCAG
AATTATTCTAAGGGTTATCT

Downstream 100 bases:

>100_bases
TCGCAGCACTTTAAGAAAACGTAAGACAATAAAAAGCCCGTTCAATGTGACGGGCTTTTTGCTGGCTTCTGATTCACAAA
CCGGCGAGATTCAGAAAATT

Product: branched-chain amino acid permease

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNQTLKLTDIIAVGFMLFAFFLGAGNIIFPPLAGQLAGEHLFPAMSGFLVTAVGLPLITIIAIAVAGGSWDHLTKDLPKQ
AATLMAVLIFIIIGPAFAAPRTGLVAYEMAVKPFFVDALQSHLTIFSILFFAVAMFFSWSQGKLIDIIGKVLTPALFVGL
IILALAVFIDPQGEMLAAQGEYLTQPLTKGFLEGYNTMDTFGSLMFGMLIVDALRSRGITERSATTKYLIYAGFIAAAGL
AFVYISLFYLGATSAAVASGADNGGVVLSLYVQALFGPYGQIVLSVIVLLACLTTAIGLISACSDYFSSLTPLSYKAWVV
INGVACAVVANVGLAQLIALSVPVLFALYPVAIALVALTFVRKKLPNPQVAYRVVILVSLLFALIDAAKVAGVDVSAFKV
LPLFEVGMAWLLPTMTAIVCMFFVSRSVRQELAEEAA

Sequences:

>Translated_437_residues
MNQTLKLTDIIAVGFMLFAFFLGAGNIIFPPLAGQLAGEHLFPAMSGFLVTAVGLPLITIIAIAVAGGSWDHLTKDLPKQ
AATLMAVLIFIIIGPAFAAPRTGLVAYEMAVKPFFVDALQSHLTIFSILFFAVAMFFSWSQGKLIDIIGKVLTPALFVGL
IILALAVFIDPQGEMLAAQGEYLTQPLTKGFLEGYNTMDTFGSLMFGMLIVDALRSRGITERSATTKYLIYAGFIAAAGL
AFVYISLFYLGATSAAVASGADNGGVVLSLYVQALFGPYGQIVLSVIVLLACLTTAIGLISACSDYFSSLTPLSYKAWVV
INGVACAVVANVGLAQLIALSVPVLFALYPVAIALVALTFVRKKLPNPQVAYRVVILVSLLFALIDAAKVAGVDVSAFKV
LPLFEVGMAWLLPTMTAIVCMFFVSRSVRQELAEEAA
>Mature_437_residues
MNQTLKLTDIIAVGFMLFAFFLGAGNIIFPPLAGQLAGEHLFPAMSGFLVTAVGLPLITIIAIAVAGGSWDHLTKDLPKQ
AATLMAVLIFIIIGPAFAAPRTGLVAYEMAVKPFFVDALQSHLTIFSILFFAVAMFFSWSQGKLIDIIGKVLTPALFVGL
IILALAVFIDPQGEMLAAQGEYLTQPLTKGFLEGYNTMDTFGSLMFGMLIVDALRSRGITERSATTKYLIYAGFIAAAGL
AFVYISLFYLGATSAAVASGADNGGVVLSLYVQALFGPYGQIVLSVIVLLACLTTAIGLISACSDYFSSLTPLSYKAWVV
INGVACAVVANVGLAQLIALSVPVLFALYPVAIALVALTFVRKKLPNPQVAYRVVILVSLLFALIDAAKVAGVDVSAFKV
LPLFEVGMAWLLPTMTAIVCMFFVSRSVRQELAEEAA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=444, Percent_Identity=41.8918918918919, Blast_Score=345, Evalue=4e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46340; Mature: 46340

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]