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The map label for this gene is rnhA [H]

Identifier: 37678700

GI number: 37678700

Start: 503675

End: 504112

Strand: Direct

Name: rnhA [H]

Synonym: VV0516

Alternate gene names: 37678700

Gene position: 503675-504112 (Clockwise)

Preceding gene: 37678699

Following gene: 37678701

Centisome position: 15.01

GC content: 45.66

Gene sequence:

>438_bases
ATGAGCTATTCAATTTACGTAGACGGCGCTGCACCAAACAACCAACACGGATGTACACGAGGTGGTATTGGGCTGGTGGT
TATGGATGAAGACAATGAGATTGTTCATGAAGAGAGCTTTACCATCAATCGAAAAACCGACAATGCTGAGCTGGAATTAC
TGGCCTTAATTGAAGCATTGGAATACGCAGAAGATGGGGATGTTATCTATTCGGACAGCGATTACTGTGTGAAAGGTTTC
AACATCTGGATGGACGATTGGAAAGACCGATGTTGGCGCCGTGCGGATAAGAAACCCGTCAAGAATCGTCACCTGTGGCA
ACAAGTCGATGAGCTGAGTTCCAGGAAGTATGTTGAAGTTGAAAAGGTAAAAGCACACTCTGGTGTTGAAGGAAATGAGA
GAGCTGACTTGTTAGCGGTTGAAGCCGCGGCGTTATAA

Upstream 100 bases:

>100_bases
CCAGAATCCTTTCAGTAACACAAACATCAAATCACATCATCAACGGGGCGCTGGCTAAGCAAAGTCATGCGCCCTTTTTA
CAATTAGAGAGAGATAAAAA

Downstream 100 bases:

>100_bases
GGTCAGTGGCACGCAGCTTGTGGGATGTACCCCACAAGCTGTCATTCTTATTTATCATTACTATTGCTGGCTTCGAGAGT
TTCTCTTAACGTTTCAGTAT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MSYSIYVDGAAPNNQHGCTRGGIGLVVMDEDNEIVHEESFTINRKTDNAELELLALIEALEYAEDGDVIYSDSDYCVKGF
NIWMDDWKDRCWRRADKKPVKNRHLWQQVDELSSRKYVEVEKVKAHSGVEGNERADLLAVEAAAL

Sequences:

>Translated_145_residues
MSYSIYVDGAAPNNQHGCTRGGIGLVVMDEDNEIVHEESFTINRKTDNAELELLALIEALEYAEDGDVIYSDSDYCVKGF
NIWMDDWKDRCWRRADKKPVKNRHLWQQVDELSSRKYVEVEKVKAHSGVEGNERADLLAVEAAAL
>Mature_144_residues
SYSIYVDGAAPNNQHGCTRGGIGLVVMDEDNEIVHEESFTINRKTDNAELELLALIEALEYAEDGDVIYSDSDYCVKGFN
IWMDDWKDRCWRRADKKPVKNRHLWQQVDELSSRKYVEVEKVKAHSGVEGNERADLLAVEAAAL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Escherichia coli, GI1786408, Length=141, Percent_Identity=39.7163120567376, Blast_Score=86, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI115532542, Length=147, Percent_Identity=31.2925170068027, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=31.2925170068027, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=31.2925170068027, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16493; Mature: 16362

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSIYVDGAAPNNQHGCTRGGIGLVVMDEDNEIVHEESFTINRKTDNAELELLALIEAL
CCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEECCCEEEECCCCCCHHHHHHHHHHH
EYAEDGDVIYSDSDYCVKGFNIWMDDWKDRCWRRADKKPVKNRHLWQQVDELSSRKYVEV
HCCCCCCEEECCCCEEEECEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEE
EKVKAHSGVEGNERADLLAVEAAAL
EEECCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
SYSIYVDGAAPNNQHGCTRGGIGLVVMDEDNEIVHEESFTINRKTDNAELELLALIEAL
CEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEECCCEEEECCCCCCHHHHHHHHHHH
EYAEDGDVIYSDSDYCVKGFNIWMDDWKDRCWRRADKKPVKNRHLWQQVDELSSRKYVEV
HCCCCCCEEECCCCEEEECEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEE
EKVKAHSGVEGNERADLLAVEAAAL
EEECCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA