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The map label for this gene is mpl [H]

Identifier: 37678619

GI number: 37678619

Start: 425648

End: 427102

Strand: Direct

Name: mpl [H]

Synonym: VV0435

Alternate gene names: 37678619

Gene position: 425648-427102 (Clockwise)

Preceding gene: 37678615

Following gene: 37678620

Centisome position: 12.69

GC content: 50.03

Gene sequence:

>1455_bases
ATGTGGCACAAGGCCAACAATGCTGCTCGCTTTTCTAACGGTTATCATGATAATGAGAACGAGAAAAAACAGTCTGATGG
CAGGATTCAATTTATGCATATTCATATCTTGGGAATTTGTGGCACTTTCATGGGTGGTGCTGCGATTTTAGCGCGACAGT
TGGGCCACAAAGTGACCGGCTCTGACGCCAATGTTTACCCACCAATGAGCACCTTGCTCGAGTCTCAAGGTATTGAAATT
ATTGAAGGCTTTGATCCTTCGCAACTTAATCCTGCGCCAGACTTGGTGGTGATTGGTAATGCCATGAGCCGGGGTAACCC
TTGTGTTGAATATGTGTTGAATCACAACCTGCGTTACACCTCTGGGCCGCAGTGGCTGCAGGAGTTTTTGTTGCATGACC
GCTGGGTATTGGCGGTATCGGGCACACATGGCAAAACCACTACCTCGAGCATGCTGGCGTGGATTTTGGAAGATTGTGGT
TACCAGCCGGGATTTCTTGTTGGTGGCGTGCTTGGTAACTTCGGTGTCTCCGCGCGTTTAGGCGAAAGCATGTTTTTTGT
CGTCGAAGCCGACGAATATGACAGTGCTTTTTTCGACAAGCGATCTAAGTTTGTGCATTACCATCCTCGCACCTTGATCA
TGAATAACCTAGAGTTTGATCATGCGGACATCTTTGATGATCTTGAAGCGATCAAGCGACAATTCCACCACTTAGTGCGT
ACTGTTCCCGGTAATGGCCGTATTTTGGCGCCCAAGCAAGACGGCGCATTAACGGATGTGTTGTCACGAGGCTGCTGGAG
TGACCAGGAGTTTTCTGGTCAGGACGGCGAGTGGCAAGCGGTCAAACTCAGCAAAGATGGTTCACACTTTGAAGTGTGGC
TGCAGGGTGAGCAAGTGGGCACGGTGCAATGGGACTTAGTCGGTGATCATAATGTCGATAATGCGTTGATGGCCATTGCA
GCGGCGCGTCATGTGGGTGTGACGCCAGATCTGGCTTGTGAGGCACTAGGGCGCTTTATTAATACCAAGCGTCGCCTAGA
GCTGAAAGGGGAAATCAATGGCGTGGCGGTGTATGACGATTTTGCCCATCACCCAACCGCGATTGAACTGACACTTGCTG
GTTTACGTAATAAGGTGGGTGATAAGAAAATCATCGCGGTACTGGAGCCACGCTCAGCCACTATGAAACGTGGTGTGCAC
AAAGAAACTTTGGCGGCTTCACTCAAGCAGGCAGATGAAGTGTTCCTTTACCAACCAGAGTCAATTGCTTGGTCGGTGCA
GGACGTGGCGGCTCAATGTCAGCAACCGGCATTCACCAGTGATAACATTGACCAGTTTGTAGCGAACATTGCGCAGCGCG
CCGAGGCGGGAACGCAAATTTTGGTCATGAGCAATGGCGGTTTTGAGGGCATTCACGGCAAATTACTGACCGCTTTAGAG
AATTCAACGCGCTAG

Upstream 100 bases:

>100_bases
TTGTATGTCGAATGAAACCGGTTGATTACACGGAAGAGTTCGATAAAAAGTGACTGAAAAAACAGCATTTGCTTCAACCC
AACGGTAAAAACTGAGCGGC

Downstream 100 bases:

>100_bases
AAAAGACACACATTAAATGAGCATGAGAAACCAAGAAAAATCGATCACATTAGCCTTTACGGGCGCTTCTGGCGCGCCTT
ATGGGTTACGTTTGTTACAG

Product: UDP-N-acetylmuramate-alanine ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MWHKANNAARFSNGYHDNENEKKQSDGRIQFMHIHILGICGTFMGGAAILARQLGHKVTGSDANVYPPMSTLLESQGIEI
IEGFDPSQLNPAPDLVVIGNAMSRGNPCVEYVLNHNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCG
YQPGFLVGGVLGNFGVSARLGESMFFVVEADEYDSAFFDKRSKFVHYHPRTLIMNNLEFDHADIFDDLEAIKRQFHHLVR
TVPGNGRILAPKQDGALTDVLSRGCWSDQEFSGQDGEWQAVKLSKDGSHFEVWLQGEQVGTVQWDLVGDHNVDNALMAIA
AARHVGVTPDLACEALGRFINTKRRLELKGEINGVAVYDDFAHHPTAIELTLAGLRNKVGDKKIIAVLEPRSATMKRGVH
KETLAASLKQADEVFLYQPESIAWSVQDVAAQCQQPAFTSDNIDQFVANIAQRAEAGTQILVMSNGGFEGIHGKLLTALE
NSTR

Sequences:

>Translated_484_residues
MWHKANNAARFSNGYHDNENEKKQSDGRIQFMHIHILGICGTFMGGAAILARQLGHKVTGSDANVYPPMSTLLESQGIEI
IEGFDPSQLNPAPDLVVIGNAMSRGNPCVEYVLNHNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCG
YQPGFLVGGVLGNFGVSARLGESMFFVVEADEYDSAFFDKRSKFVHYHPRTLIMNNLEFDHADIFDDLEAIKRQFHHLVR
TVPGNGRILAPKQDGALTDVLSRGCWSDQEFSGQDGEWQAVKLSKDGSHFEVWLQGEQVGTVQWDLVGDHNVDNALMAIA
AARHVGVTPDLACEALGRFINTKRRLELKGEINGVAVYDDFAHHPTAIELTLAGLRNKVGDKKIIAVLEPRSATMKRGVH
KETLAASLKQADEVFLYQPESIAWSVQDVAAQCQQPAFTSDNIDQFVANIAQRAEAGTQILVMSNGGFEGIHGKLLTALE
NSTR
>Mature_484_residues
MWHKANNAARFSNGYHDNENEKKQSDGRIQFMHIHILGICGTFMGGAAILARQLGHKVTGSDANVYPPMSTLLESQGIEI
IEGFDPSQLNPAPDLVVIGNAMSRGNPCVEYVLNHNLRYTSGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCG
YQPGFLVGGVLGNFGVSARLGESMFFVVEADEYDSAFFDKRSKFVHYHPRTLIMNNLEFDHADIFDDLEAIKRQFHHLVR
TVPGNGRILAPKQDGALTDVLSRGCWSDQEFSGQDGEWQAVKLSKDGSHFEVWLQGEQVGTVQWDLVGDHNVDNALMAIA
AARHVGVTPDLACEALGRFINTKRRLELKGEINGVAVYDDFAHHPTAIELTLAGLRNKVGDKKIIAVLEPRSATMKRGVH
KETLAASLKQADEVFLYQPESIAWSVQDVAAQCQQPAFTSDNIDQFVANIAQRAEAGTQILVMSNGGFEGIHGKLLTALE
NSTR

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=449, Percent_Identity=69.0423162583519, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1786279, Length=302, Percent_Identity=30.1324503311258, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 53407; Mature: 53407

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWHKANNAARFSNGYHDNENEKKQSDGRIQFMHIHILGICGTFMGGAAILARQLGHKVTG
CCCCCCCCHHCCCCCCCCCCCHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCC
SDANVYPPMSTLLESQGIEIIEGFDPSQLNPAPDLVVIGNAMSRGNPCVEYVLNHNLRYT
CCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCC
SGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCGYQPGFLVGGVLGNFGVSARL
CCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCEEC
GESMFFVVEADEYDSAFFDKRSKFVHYHPRTLIMNNLEFDHADIFDDLEAIKRQFHHLVR
CCEEEEEEECCCCCHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TVPGNGRILAPKQDGALTDVLSRGCWSDQEFSGQDGEWQAVKLSKDGSHFEVWLQGEQVG
CCCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCEEC
TVQWDLVGDHNVDNALMAIAAARHVGVTPDLACEALGRFINTKRRLELKGEINGVAVYDD
EEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEECC
FAHHPTAIELTLAGLRNKVGDKKIIAVLEPRSATMKRGVHKETLAASLKQADEVFLYQPE
CCCCCCEEEEEEHHHHCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCEEEECCC
SIAWSVQDVAAQCQQPAFTSDNIDQFVANIAQRAEAGTQILVMSNGGFEGIHGKLLTALE
HHEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
NSTR
CCCC
>Mature Secondary Structure
MWHKANNAARFSNGYHDNENEKKQSDGRIQFMHIHILGICGTFMGGAAILARQLGHKVTG
CCCCCCCCHHCCCCCCCCCCCHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCC
SDANVYPPMSTLLESQGIEIIEGFDPSQLNPAPDLVVIGNAMSRGNPCVEYVLNHNLRYT
CCCCCCCCHHHHHHHCCCEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCC
SGPQWLQEFLLHDRWVLAVSGTHGKTTTSSMLAWILEDCGYQPGFLVGGVLGNFGVSARL
CCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCEEC
GESMFFVVEADEYDSAFFDKRSKFVHYHPRTLIMNNLEFDHADIFDDLEAIKRQFHHLVR
CCEEEEEEECCCCCHHHHHCCCCEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
TVPGNGRILAPKQDGALTDVLSRGCWSDQEFSGQDGEWQAVKLSKDGSHFEVWLQGEQVG
CCCCCCEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCEEC
TVQWDLVGDHNVDNALMAIAAARHVGVTPDLACEALGRFINTKRRLELKGEINGVAVYDD
EEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCEEEECC
FAHHPTAIELTLAGLRNKVGDKKIIAVLEPRSATMKRGVHKETLAASLKQADEVFLYQPE
CCCCCCEEEEEEHHHHCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHCCCCEEEECCC
SIAWSVQDVAAQCQQPAFTSDNIDQFVANIAQRAEAGTQILVMSNGGFEGIHGKLLTALE
HHEEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
NSTR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]