Click here to switch to the map view.

The map label for this gene is fcl [H]

Identifier: 37678534

GI number: 37678534

Start: 358588

End: 359595

Strand: Direct

Name: fcl [H]

Synonym: VV0350

Alternate gene names: 37678534

Gene position: 358588-359595 (Clockwise)

Preceding gene: 37678533

Following gene: 37678535

Centisome position: 10.69

GC content: 38.99

Gene sequence:

>1008_bases
ATGATAACCCAGCTTATGCTGGATTTTATTATGGATAAAGAAATGAGTTCTAAAACAGTTTTTGTCGCAGGCCATACCGG
TATGGTAGGCAGTGCAATTGTTAGAAAGTTAAAGCAGTCTAATGATATTAAGGTTATTACAAAAAGCAGAGCTGAGTTAA
ACCTGTTGGACCAGCAAGCAGTTCGTACATTTTTTGAAGAGAACCAAATAGACCAAGTATATCTTGCTGCGGCGAAAGTG
GGCGGGATTGTAGCGAACAATACTTACCCAGCAGAATTCATTTATGAGAATTTAACAATTCAAAGTAACATTATCCATAG
TGCACATTTATCAGGCGTTAATGACTTACTGTTTTTAGGATCAAGTTGTATTTACCCTAAATTCGCAGAGCAGCCAATGA
CAGAAACAGCACTGTTAACTGGGGTGTTAGAGCCAACCAATGAGCCTTATGCGATAGCTAAAATTGCAGGTATAAAACTG
TGTGAATCTTATAACCGACAATATGGTCATAATTATCGCTCAGTCATGCCAACTAATCTGTATGGCGAAAATGATAACTT
CCACCCAGAAAACTCTCATGTTATTCCAGCGTTAATACGTCGTTTTCATGAGGCGAAATTAGCAGGTGATGGCAAAGTGG
TTGCGTGGGGCACTGGTAAGCCGAGGCGAGAGTTTTTGCATGTAAACGATATGGCTGAAGCATCAATTCATGTTATGAAC
TTAGATTCAAAAAAATACAGTGTAAATACGCAAGAGATGTTGAGCCATATAAATGTCGGTACCGGTGTAGATTGTACAAT
TAGAGAGCTTGTTGAAACGGTTGCAAAAGTCGTTGGTTTTGAAGGTGTAATTGAGTTTGATGTCACAAAGCCAGACGGAA
CACCACGTAAACTTATGGATGTGTCTCGTCTCAAGTCACTGGGCTGGGAATACAGTATTTCTTTAGAGGTAGGTCTAAGA
GATACGTATGGATGGTTTTTAGCAAACCAAGATAATTTTAGAAAATAG

Upstream 100 bases:

>100_bases
GACTTAGCTAAAGCGAAGCAGCATGCATTATTGAAATCTCACGGCTACTCTGTGAGTGTTCCAGTAGAGTAATCTTAGAG
CAGTAGGTCAGAAATAAAAA

Downstream 100 bases:

>100_bases
AGAACATAGGATGTTTCTAGATAAACGTACGTTTTCGACAGTAATAGAAAGCACGCCACTCATTTCAATCGATTTGGTTA
TATTTGATCAAAATAATCAA

Product: putative nucleotide di-P-sugar epimerase or dehydratase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MITQLMLDFIMDKEMSSKTVFVAGHTGMVGSAIVRKLKQSNDIKVITKSRAELNLLDQQAVRTFFEENQIDQVYLAAAKV
GGIVANNTYPAEFIYENLTIQSNIIHSAHLSGVNDLLFLGSSCIYPKFAEQPMTETALLTGVLEPTNEPYAIAKIAGIKL
CESYNRQYGHNYRSVMPTNLYGENDNFHPENSHVIPALIRRFHEAKLAGDGKVVAWGTGKPRREFLHVNDMAEASIHVMN
LDSKKYSVNTQEMLSHINVGTGVDCTIRELVETVAKVVGFEGVIEFDVTKPDGTPRKLMDVSRLKSLGWEYSISLEVGLR
DTYGWFLANQDNFRK

Sequences:

>Translated_335_residues
MITQLMLDFIMDKEMSSKTVFVAGHTGMVGSAIVRKLKQSNDIKVITKSRAELNLLDQQAVRTFFEENQIDQVYLAAAKV
GGIVANNTYPAEFIYENLTIQSNIIHSAHLSGVNDLLFLGSSCIYPKFAEQPMTETALLTGVLEPTNEPYAIAKIAGIKL
CESYNRQYGHNYRSVMPTNLYGENDNFHPENSHVIPALIRRFHEAKLAGDGKVVAWGTGKPRREFLHVNDMAEASIHVMN
LDSKKYSVNTQEMLSHINVGTGVDCTIRELVETVAKVVGFEGVIEFDVTKPDGTPRKLMDVSRLKSLGWEYSISLEVGLR
DTYGWFLANQDNFRK
>Mature_335_residues
MITQLMLDFIMDKEMSSKTVFVAGHTGMVGSAIVRKLKQSNDIKVITKSRAELNLLDQQAVRTFFEENQIDQVYLAAAKV
GGIVANNTYPAEFIYENLTIQSNIIHSAHLSGVNDLLFLGSSCIYPKFAEQPMTETALLTGVLEPTNEPYAIAKIAGIKL
CESYNRQYGHNYRSVMPTNLYGENDNFHPENSHVIPALIRRFHEAKLAGDGKVVAWGTGKPRREFLHVNDMAEASIHVMN
LDSKKYSVNTQEMLSHINVGTGVDCTIRELVETVAKVVGFEGVIEFDVTKPDGTPRKLMDVSRLKSLGWEYSISLEVGLR
DTYGWFLANQDNFRK

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=329, Percent_Identity=31.6109422492401, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI7657641, Length=288, Percent_Identity=23.2638888888889, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788365, Length=320, Percent_Identity=70.625, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI17554428, Length=322, Percent_Identity=29.5031055900621, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19922778, Length=327, Percent_Identity=31.4984709480122, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21356223, Length=344, Percent_Identity=24.7093023255814, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 37499; Mature: 37499

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQLMLDFIMDKEMSSKTVFVAGHTGMVGSAIVRKLKQSNDIKVITKSRAELNLLDQQA
CHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
VRTFFEENQIDQVYLAAAKVGGIVANNTYPAEFIYENLTIQSNIIHSAHLSGVNDLLFLG
HHHHHHCCCCCHHEEEHHHHCCEEECCCCCHHHHHCCCEEEHHHHHHHHHCCHHHHHHHC
SSCIYPKFAEQPMTETALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGHNYRSVMPTNL
CCCCCCCHHCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
YGENDNFHPENSHVIPALIRRFHEAKLAGDGKVVAWGTGKPRREFLHVNDMAEASIHVMN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEECCCCCCEEEEEE
LDSKKYSVNTQEMLSHINVGTGVDCTIRELVETVAKVVGFEGVIEFDVTKPDGTPRKLMD
CCCCCEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
VSRLKSLGWEYSISLEVGLRDTYGWFLANQDNFRK
HHHHHHCCCEEEEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MITQLMLDFIMDKEMSSKTVFVAGHTGMVGSAIVRKLKQSNDIKVITKSRAELNLLDQQA
CHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
VRTFFEENQIDQVYLAAAKVGGIVANNTYPAEFIYENLTIQSNIIHSAHLSGVNDLLFLG
HHHHHHCCCCCHHEEEHHHHCCEEECCCCCHHHHHCCCEEEHHHHHHHHHCCHHHHHHHC
SSCIYPKFAEQPMTETALLTGVLEPTNEPYAIAKIAGIKLCESYNRQYGHNYRSVMPTNL
CCCCCCCHHCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCC
YGENDNFHPENSHVIPALIRRFHEAKLAGDGKVVAWGTGKPRREFLHVNDMAEASIHVMN
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHEEEECCCCCCEEEEEE
LDSKKYSVNTQEMLSHINVGTGVDCTIRELVETVAKVVGFEGVIEFDVTKPDGTPRKLMD
CCCCCEECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
VSRLKSLGWEYSISLEVGLRDTYGWFLANQDNFRK
HHHHHHCCCEEEEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]